How is nuclear size regulated relative to cell size? Using microfluidic encapsulation of Xenopus laevis embryo extracts, biochemical fractionation, and in vivo experiments, Chen et al. demonstrate that reductions in cytoplasmic volume and limiting components, including the histone chaperone nucleoplasmin, contribute to developmental nuclear size scaling.

Baumgart et al. measure the amount of dimeric and polymeric tubulin at mitotic centrosomes in C. elegans by light and electron microscopy. Centrosomes concentrate soluble tubulin tenfold over the cytoplasm, suggesting that centrosomal microtubule nucleation may be driven in part by concentrating tubulin.

Wang et al. identify Rab7 as a novel regulator of primary cilia disassembly. Their findings demonstrate that Rab7 localization to primary cilia is required for intraciliary F-actin polymerization, which is indispensable for the regulation of cilia ectocytosis and disassembly.