Newest Articles
Cdk1 phosphorylation controls Nde1 cargo bindingNde1 plays a role in recruitment of cytoplasmic dynein to cargo. This study finds that Cdk1 phosphorylation of Nde1 enhances its recruitment by CENP-F. This interaction controls Nde1 and, in turn, dynein levels at the G2 nuclear envelope and mitotic kinetochores from prophase through anaphase.
Cytosolic chaperones and β-barrel proteinsMitochondrial β-barrel proteins are imported from the cytosol into the organelle. Jores et al. provide new insights into the early events of this process by describing an array of cytosolic chaperones and cochaperones that associate with newly synthesized β-barrel proteins and assure their optimal biogenesis.
Cell cycle–dependent regulation of adhesionAdherent cells round up before dividing, but how this is linked to the cell cycle is unclear. Jones et al. demonstrate that CDK1 promotes adhesion complex formation and increases cell adhesion area from G1 to S phase. Subsequently, inhibitory phosphorylation of CDK1 by cyclin B1 triggers adhesion complex disassembly during G2 in preparation for mitosis.
Escherichia coli HflX is a ribosome-associated RNA helicaseHflX, which was recently identified as a heat shock protein, is a putative GTPase. HflX also has ATPase activity, but the role of this is unknown. Dey at al. now reveal that HflX has ATP-dependent RNA helicase activity that is instrumental in recovering heat-inactivated 50S rRNA in Escherichia coli.
Munc13-4 regulates Ca2+-dependent exosome releaseMessenger et al. show that exosome secretion in cancer cells is Ca2+-stimulated and dependent on Ca2+-bound Munc13-4. Munc13-4, a late endosome priming factor, acts via a Rab11-dependent pathway to prepare multivesicular endosomes for exocytosis. These results indicate that Munc13-4 plays a central role in exosome release in cancer cells.
Septins suppress the spread of vacciniaSeptins play important roles in many cellular processes. Pfanzelter et al. show that septins suppress the release of vaccinia virus from infected cells by trapping virions on the plasma membrane. This antiviral effect is overcome by dynamin and formin-mediated actin polymerization.
Identifying adult mammary stem cell vulnerabilitiesCasey et al. integrate epigenomic, transcriptomic, and proteomic profiling of primary basal and luminal mammary cells to identify master epigenetic regulators of the mammary epithelium and uncover stem and progenitor cell vulnerabilities. They develop a pipeline to identify drugs that abrogate progenitor cell activity in normal and high-risk breast cancer patient samples in vitro and in vivo.
Current Issue
June 4, 2018
Volume 217, No. 6
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Reviews & Opinions
- Glutathione metabolism in cancer progression and treatment resistance
Bansal and Simon discuss strategies to block glutathione synthesis and utilization pathways to inhibit tumor propagation and treatment resistance.
- Between sisters: Watching replication-associated recombinational DNA repair
Lovett previews work from Amarh et al. describing the dynamics of RecA-mediated repair at replication-dependent breaks.
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Gerton introduces new work from Schiklenk et al. identifying the Zas1 transcription factor as a key regulator of chromosome condensation.