Pizzinga et al. show that mRNAs encoding a range of translation factors are localized to granules that get transported into the yeast daughter cell using the She2p/She3p machinery. This likely supports an intensification of protein synthetic activity to facilitate apical polarized growth.

How the nuclear envelope is remodeled to facilitate insertion of large protein complexes is poorly understood. Chen et al. use superresolution imaging with bimolecular fluorescence complementation to show that a novel noncanonical linker of nucleoskeleton and cytoskeleton (LINC) complex forms at sites of nuclear envelope fenestration in yeast.

The Golgi cisternal maturation model predicts that secretory cargo proteins should be continuously present within the cisternae while resident Golgi proteins come and go. Casler et al. verify this prediction by tracking the passage of a fluorescent secretory cargo through the yeast Golgi.