Newest Articles
Dynamics of sister chromatid resolutionStanyte et al. use dCas9-mEGFP labelling to image endogenous genomic loci and show that sister loci separate shortly after their replication and rapidly equilibrate their relative position, thereby establishing a sister chromatid resolution topology in G2 that largely reflects the DNA replication program. The data suggest that cohesin enrichment sites are not persistent cohesive sites in human cells.
Revisiting LAMP1 as a marker for neuronal lysosomeCheng et al. combine quantitative immunoelectron microscopy and light imaging microscopy to comprehensively analyze LAMP1 subcellular distribution in neurons. The data show that a significant portion of LAMP1-positive organelles are not degradative, illustrating the need for additional lysosomal and endocytic markers to define endolysosomal compartments in the nervous system.
Nucleolar 60S ribosomal subunit remodeling eventsRibosome biogenesis involves numerous pre-rRNA processing events to remove internal and external transcribed spacer sequences, ultimately yielding three mature rRNAs. Biedka et al. show that ribosomal proteins and assembly factors remodel several neighborhoods, including two 60S ribosomal subunit functional centers, during removal of the ITS2 spacer RNA.
PI(4,5)P2 enrichment regulates the mechanical force–induced B cell activationAntigens deliver mechanical forces to B cell receptors (BCRs) to initiate B cell activation. Using mechanical force biosensors, Wan et al. show that the enrichment of PI(4,5)P2 within the IgG-BCR membrane microdomain determines the threshold of mechanical force–induced B cell activation.
Kif18b walks to microtubule ends and shortens themKinesin-8 Kif18b shortens astral microtubules in mitosis. Combining cell biology and biochemical reconstitution, McHugh et al. show that Kif18b walks and accumulates to microtubule plus ends in a phosphospecific manner to regulate astral microtubule dynamics and center the mitotic spindle.
Chaperones manage misfolded proteins in the nucleusQuality control (QC) pathways for misfolded proteins depend on E3 ubiquitin ligases and associated chaperones. Prasad et al. show that Hsp40/70/110 chaperones traffic and manage misfolded proteins in the nucleus, extending the nuclear protein QC pathway to include cytosolic clients.
Lymphatic exosomes enhance directional migrationInflammation stimulates lymphatic endothelial cells to release exosomes, which accumulate in the perivascular stroma. Brown et al. show that these exosomes promote the directional migration of dendritic cells along guidance cues in complex environments by enhancing dynamic cellular protrusions in a CX3CL1-dependent manner.
Current Issue
April 2, 2018
Volume 217, No. 4
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Reviews & Opinions
- Modulation of the secretory pathway by amino-acid starvation
van Leeuwen et al. discuss how the cellular secretory pathway adapts and contributes to stress-induced pathways during nutrient starvation.
- Superoxide dismutases: Dual roles in controlling ROS damage and regulating ROS signaling
Wang et al. review the dual role of superoxide dismutases in controlling reactive oxygen species (ROS) damage and regulating ROS signaling across model systems as well as their involvement in human diseases.
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Hoefig and Heissmeyer review how microRNAs, long noncoding RNAs, RNA-binding proteins, and ubiquitin-modifying enzymes regulate T helper cell differentiation downstream of transcription.