Newest Articles
Cryo–electron microscopy structure of seipinSui et al. report the cryo-EM structure of the conserved luminal domain of the lipid droplet (LD)-formation protein seipin. The structure reveals key features of this domain and suggests a new model for seipin’s role in LD formation.
Translating and nontranslating mRNP architectureKhong and Parker use single-molecule FISH to examine the timing of mRNA entry to stress granule as well as mRNA protein complex (mRNP) architecture. mRNA compaction increases after ribosome runoff, suggesting that mRNPs preferentially adopt a closed-loop structure in nontranslating conditions.
β-Catenin stability is decreased at high pHWhite et al. find that intracellular pH regulates the stability of β-catenin, the Wnt signaling molecule that controls cell polarity, adhesion, and differentiation. A conserved histidine residue in β-catenin mediates pH-dependent binding to the E3 ligase β-TrCP for degradation, and a cancer-associated mutation that bypasses this pH-sensitive regulation induces ectopic tumors in the Drosophila eye.
PP2A-B55 in nuclear envelope reformationMultiple proteins are dephosphorylated when mitosis finishes. Although the phosphatase PP2A-B55 promotes this process, its key substrates and functions are not clear. Mehsen et al. conducted a genetic screen in Drosophila that pointed to a role for PP2A-B55 in nuclear envelope reformation and its dephosphorylation of BAF as a crucial event.
SNARE mimicry by SipA promotes membrane fusionIntracellular pathogens can modulate host Rabs and SNAREs to support their replication and immune evasion. Singh et al. show that the Salmonella effector SipA functionally mimics an R-SNARE and recruits host Q-SNAREs to promote membrane fusion. Thus, SNARE mimicry by this intracellular pathogen effector modulates the host trafficking machinery for Salmonella survival.
Cooperative binding of kinesin-1 to microtubulesShima et al. show that there is a positive cooperativity in the binding of kinesin-1 to GDP microtubules. Binding of kinesin-1 triggers conformational changes in GDP microtubules, which then take a GMPCPP-microtubule–like conformation. These effects require microtubule to bind with kinesin in the nucleotide-free state.
Stem cell aneuploidy promotes gut dysplasiaChromosome number is highly regulated in cells, while genome instability is associated with cancer. Resende et al. show that induction of chromosome imbalance in stem cells of the fruit fly adult gut leads to tissue pathology highly reminiscent of early stages of human tumor development.
Current Issue
October 1, 2018
Volume 217, No. 10
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Reviews & Opinions
- Histone supply: Multitiered regulation ensures chromatin dynamics throughout the cell cycle
Mendiratta et al. review the interplay between the different regulatory layers that affect the transcription and dynamics of distinct histone H3 variants along the cell cycle.
- Genomic insights into chromatin reprogramming to totipotency in embryos
Ladstätter and Tachibana discuss changes in DNA methylation, chromatin accessibility, and topological architecture occurring during the reprogramming to totipotency in the early embryo.
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Philip and Harrison introduce research from Condon et al., who use high resolution imaging to reveal novel surface structures involved in macropinocytosis.