Newest Articles
SRC inhibitors reactivate the tumor suppressor DLC1DLC1 controls focal adhesion dynamics and other processes that suppress tumorigenesis; therefore, it is unclear why some cancers maintain high levels of DLC1. Tripathi et al. show that phosphorylation of DLC1 by SRC and ERK mitigates DLC1’s tumor suppressor activities but these can be reactivated by kinase inhibition as a potential cancer treatment.
The exosome regulates hESC differentiationThis work shows that the exosome modulates the levels of LINE-1 retrotransposons and specific miRNAs, lncRNAs, and mRNAs that encode developmental regulators or affect their expression. The exosome restrains stem cell differentiation in part by degrading transcripts encoding FOXH1, a transcription factor crucial for mesendoderm formation.
Quantitative glycoproteomics of OST mutantsCherepanova et al. provide quantitative glycoproteomic analyses of human cells that lack either the STT3A or STT3B oligosaccharyltransferase (OST) complex, revealing new classes of STT3A- and STT3B-dependent glycosylation sites and indicating how cooperation between the OST complexes maximizes acceptor site occupancy in cellular glycoproteins.
High-throughput electron microscopyBykov et al. describe a method called “MultiCLEM” to screen ultrastructural phenotypes by high-throughput electron microscopy (EM). The method uses combinations of fluorophores as barcodes to identify cells. It is applied to study multivesicular bodies, mitochondria, and peroxisomes.
Spindle maturation in oocytesCosta and Ohkura provide new insight into how the bipolar spindle is stably maintained during metaphase arrest in oocytes. They find that many important spindle proteins change their localization during arrest in Drosophila oocytes and show the importance of these changes in stabilizing the spindle during arrest.
Linkages between heterologous chromosomesPotapova et al. use superresolution microscopy to describe linkages between ribosomal DNA on heterologous human chromosomes whose formation depends on the transcription factor UBF and topoisomerase II. Linkages persist in the absence of cohesion but require topoisomerase II for resolution.
A Drg–Dvl interaction regulates ciliogenesisOur study reveals Drg1 as a new binding partner of Dishevelled. The Drg1–Dishevelled association regulates Daam1 and RhoA interactions and activity, leading to polymerization and stability of the actin cytoskeleton, a process that is essential for proper multiciliation.
Current Issue
July 1, 2019
Volume 218, No. 7
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Reviews & Opinions
- Clathrin-containing adhesion complexes
Lock et al. offer a perspective on how the discovery of a new class of cell adhesion complex drives convergence of the integrin and clathrin fields.
- Loss of nuclear envelope integrity? No probLEM—BAF has it covered
King and Lusk preview work from Halfmann et al. that provides insight into the mechanisms that mediate the repair of nuclear envelope rupture.
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W.M. Henne previews work from Chang et al. that demonstrates a role for Spastin in tethering lipid droplets to peroxisomes to facilitate fatty acid exchange.