Heparan sulfate proteoglycans from mouse mammary epithelial cells: localization on the cell surface with a monoclonal antibody

doi:10.1083/jcb.101.3.976

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Heparan sulfate proteoglycans from mouse mammary epithelial cells: localization on the cell surface with a monoclonal antibody

M Jalkanen, H Nguyen, A Rapraeger, N Kurn and M Bernfield

Mouse mammary epithelial cells, of the normal murine mammary gland (NMuMG) cell line, bear a heparan sulfate-rich proteoglycan (HSPG) on their surfaces. A hybridoma (281-2) secreting a monoclonal antibody that recognizes this HSPG was produced by fusion of SP-2/0 myeloma cells with spleen cells from rats immunized with NMuMG cells. The 281-2 monoclonal antibody is directed against the core protein of the cell surface HSPG, as demonstrated by (a) recognition of the isolated proteoglycan but not its glycosaminoglycan chains, (b) co-localization of 281-2-specific antigen and radioactive cell surface HSPG on gradient polyacrylamide gel electrophoresis and on isopycnic centrifugation, and (c) abolition of immunofluorescent staining of the NMuMG cell surface by the intact, but not the protease-digested ectodomain of the cell surface HSPG. The antibody is specific for cell surface HSPG and does not recognize the HSPG that accumulates extracellularly beneath the basal cell surface. Therefore, the 281-2 antibody may be used to isolate the cell surface HSPG and to explore its distribution in tissues.
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Yang, Y., Borset, M., Langford, J. K., Sanderson, R. D. (2003). Heparan Sulfate Regulates Targeting of Syndecan-1 to a Functional Domain on the Cell Surface. J. Biol. Chem. 278: 12888-12893 [Abstract] [Full Text]
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Soro, P. G., Morales-A., P., Martinez-M., J. A., Morales-A., S., Copin, S. G., Marcos, M. A. R., Gaspar, M.-L. (1999). Differential Involvement of the Transcription Factor Blimp-1 in T Cell-Independent and -Dependent B Cell Differentiation to Plasma Cells. J. Immunol. 163: 611-617 [Abstract] [Full Text]
Romaris, M., Coomans, C., Ceulemans, H., Bruystens, A.-M., Vekemans, S., David, G. (1999). Molecular Polymorphism of the Syndecans. IDENTIFICATION OF A HYPO-GLYCANATED MURINE SYNDECAN-1 SPLICE VARIANT. J. Biol. Chem. 274: 18667-18674 [Abstract] [Full Text]
Maatta, A., Jaakkola, P., Jalkanen, M. (1999). Extracellular Matrix-dependent Activation of Syndecan-1 Expression in Keratinocyte Growth Factor-treated Keratinocytes. J. Biol. Chem. 274: 9891-9898 [Abstract] [Full Text]
Ott, V. L., Rapraeger, A. C. (1998). Tyrosine Phosphorylation of Syndecan-1 and -4�Cytoplasmic Domains in Adherent B82 Fibroblasts. J. Biol. Chem. 273: 35291-35298 [Abstract] [Full Text]
Liu, W., Litwack, E. D., Stanley, M. J., Langford, J. K., Lander, A. D., Sanderson, R. D. (1998). Heparan Sulfate Proteoglycans as Adhesive and Anti-invasive Molecules. SYNDECANS AND GLYPICAN HAVE DISTINCT FUNCTIONS. J. Biol. Chem. 273: 22825-22832 [Abstract] [Full Text]
Dhodapkar, M. V., Abe, E., Theus, A., Lacy, M., Langford, J. K., Barlogie, B., Sanderson, R. D. (1998). Syndecan-1 Is a Multifunctional Regulator of Myeloma Pathobiology: Control of Tumor Cell Survival, Growth, and Bone Cell Differentiation. Blood 91: 2679-2688 [Abstract] [Full Text]
Tkachuk, M., Bolliger, S., Ryffel, B., Pluschke, G., Banks, T. A., Herren, S., Gisler, R. H., Kosco-Vilbois, M. H. (1998). Crucial Role of Tumor Necrosis Factor Receptor 1 Expression on Nonhematopoietic Cells for B Cell Localization within the Splenic White Pulp. JEM 187: 469-477 [Abstract] [Full Text]
Subramanian, S. V., Fitzgerald, M. L., Bernfield, M. (1997). Regulated Shedding of Syndecan-1 and -4�Ectodomains by Thrombin and Growth Factor Receptor Activation. J. Biol. Chem. 272: 14713-14720 [Abstract] [Full Text]
Maas, R., Bei, M. (1997). The Genetic Control of Early Tooth Development. CROBM 8: 4-39 [Abstract] [Full Text]
Williamson, T. G., Mok, S. S., Henry, A., Cappai, R., Lander, A. D., Nurcombe, V., Beyreuther, K., Masters, C. L., Small, D. H. (1996). Secreted Glypican Binds to the Amyloid Precursor Protein of Alzheimer's Disease (APP) and Inhibits APP-induced Neurite Outgrowth. J. Biol. Chem. 271: 31215-31221 [Abstract] [Full Text]
Kainulainen, V., Nelimarkka, L., Jarvelainen, H., Laato, M., Jalkanen, M., Elenius, K. (1996). Suppression of Syndecan-1 Expression in Endothelial Cells by Tumor Necrosis Factor-alpha. J. Biol. Chem. 271: 18759-18766 [Abstract] [Full Text]
Leppa, S, Vleminckx, K, Van Roy, F, Jalkanen, M (1996). Syndecan-1 expression in mammary epithelial tumor cells is E-cadherin-dependent. J. Cell Sci. 109: 1393-1403 [Abstract]
Stanley, M. J., Liebersbach, B. F., Liu, W., Anhalt, D. J., Sanderson, R. D. (1995). Heparan Sulfate-mediated Cell Aggregation. J. Biol. Chem. 270: 5077-5083 [Abstract] [Full Text]
Mitsiadis, T., Salmivirta, M, Muramatsu, T, Muramatsu, H, Rauvala, H, Lehtonen, E, Jalkanen, M, Thesleff, I (1995). Expression of the heparin-binding cytokines, midkine (MK) and HB-GAM (pleiotrophin) is associated with epithelial-mesenchymal interactions during fetal development and organogenesis. Development 121: 37-51 [Abstract]
Miettinen, H., Jalkanen, M (1994). The cytoplasmic domain of syndecan-1 is not required for association with Triton X-100-insoluble material. J. Cell Sci. 107: 1571-1581 [Abstract]
Yamagata, M, Saga, S, Kato, M, Bernfield, M, Kimata, K (1993). Selective distributions of proteoglycans and their ligands in pericellular matrix of cultured fibroblasts. Implications for their roles in cell-substratum adhesion. J. Cell Sci. 106: 55-65 [Abstract]
Trelstad, R. L. (1988). The Extracellular Matrix Is a Soluble and Solid-Phase Agonist and Receptor. Arch Dermatol 124: 706-708 [Abstract]
Ueno, M., Yamada, S., Zako, M., Bernfield, M., Sugahara, K. (2001). Structural Characterization of Heparan Sulfate and Chondroitin Sulfate of Syndecan-1 Purified from Normal Murine Mammary Gland Epithelial Cells. COMMON PHOSPHORYLATION OF XYLOSE AND DIFFERENTIAL SULFATION OF GALACTOSE IN THE PROTEIN LINKAGE REGION TETRASACCHARIDE SEQUENCE. J. Biol. Chem. 276: 29134-29140 [Abstract] [Full Text]