Autoregulation of tubulin synthesis in hepatocytes and fibroblasts

doi:10.1083/jcb.101.5.1763

This Article

	Full Text (PDF, 1397K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Caron, J. M.
	Articles by Kirschner, M. W.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Caron, J. M.
	Articles by Kirschner, M. W.


Social Bookmarking

	What's this?

ARTICLES

Autoregulation of tubulin synthesis in hepatocytes and fibroblasts

JM Caron, AL Jones and MW Kirschner

Microtubule polymer levels in mouse 3T6 fibroblasts and primary cultures of rat hepatocytes can be manipulated by treatment of cells with long term, low doses of colcemid. Such treatment produces a rather uniform population of cells with microtubules of reduced lengths. Using this system, we demonstrate (a) that the rate of tubulin synthesis is sensitive to small changes (10%) in microtubule polymer mass and (b) that the percent of inhibition of synthesis is proportional to the level of soluble tubulin. Experiments with hepatocytes indicate that not only synthesis but the stability of tubulin protein was also regulated to maintain a specific level of tubulin. Treatment of hepatocytes with colcemid or other microtubule-depolymerizing drugs reduced the half-life of tubulin from 50 to 2 h, whereas taxol, which stabilizes microtubules, increased the half-life. To assess the consequences of altering microtubule polymer mass, we have analyzed the effect of controlled depolymerization of microtubules in rat hepatocytes on the processing of endocytosed ligands and found it sensitive to small changes in microtubule polymer levels.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Chen, J., Sroubek, J., Krishnan, Y., Li, Y., Bian, J., McDonald, T. V. (2009). PKA phosphorylation of HERG protein regulates the rate of channel synthesis. Am. J. Physiol. Heart Circ. Physiol. 296: H1244-H1254 [Abstract] [Full Text]
Geiger, R. C., Kaufman, C. D., Lam, A. P., Budinger, G. R. S., Dean, D. A. (2009). Tubulin Acetylation and Histone Deacetylase 6 Activity in the Lung under Cyclic Load. Am. J. Respir. Cell Mol. Bio. 40: 76-82 [Abstract] [Full Text]
Sellin, M. E., Holmfeldt, P., Stenmark, S., Gullberg, M. (2008). Global Regulation of the Interphase Microtubule System by Abundantly Expressed Op18/Stathmin. Mol. Biol. Cell 19: 2897-2906 [Abstract] [Full Text]
Fanara, P., Turner, S., Busch, R., Killion, S., Awada, M., Turner, H., Mahsut, A., LaPrade, K. L., Stark, J. M., Hellerstein, M. K. (2004). In Vivo Measurement of Microtubule Dynamics Using Stable Isotope Labeling with Heavy Water: EFFECT OF TAXANES. J. Biol. Chem. 279: 49940-49947 [Abstract] [Full Text]
Polte, T. R., Eichler, G. S., Wang, N., Ingber, D. E. (2004). Extracellular matrix controls myosin light chain phosphorylation and cell contractility through modulation of cell shape and cytoskeletal prestress. Am. J. Physiol. Cell Physiol. 286: C518-C528 [Abstract] [Full Text]
Putnam, A. J., Cunningham, J. J., Pillemer, B. B. L., Mooney, D. J. (2003). External mechanical strain regulates membrane targeting of Rho GTPases by controlling microtubule assembly. Am. J. Physiol. Cell Physiol. 284: C627-C639 [Abstract] [Full Text]
Collinsworth, A. M., Zhang, S., Kraus, W. E., Truskey, G. A. (2002). Apparent elastic modulus and hysteresis of skeletal muscle cells throughout differentiation. Am. J. Physiol. Cell Physiol. 283: C1219-C1227 [Abstract] [Full Text]
Barlow, S. B., Gonzalez-Garay, M. L., Cabral, F. (2002). Paclitaxel-dependent mutants have severely reduced microtubule assembly and reduced tubulin synthesis. J. Cell Sci. 115: 3469-3478 [Abstract] [Full Text]
Caron, J. M., Vega, L. R., Fleming, J., Bishop, R., Solomon, F. (2001). Single Site alpha -Tubulin Mutation Affects Astral Microtubules and Nuclear Positioning during Anaphase in Saccharomyces cerevisiae: Possible Role for Palmitoylation of alpha -Tubulin. Mol. Biol. Cell 12: 2672-2687 [Abstract] [Full Text]
Gilbert, S., Loranger, A., Daigle, N., Marceau, N. (2001). Simple epithelium keratins 8 and 18 provide resistance to Fas-mediated apoptosis. The protection occurs through a receptor-targeting modulation. JCB 154: 763-774 [Abstract] [Full Text]
Correa, L. M., Miller, M. G. (2001). Microtubule Depolymerization in Rat Seminiferous Epithelium Is Associated with Diminished Tyrosination of {{alpha}}-Tubulin. Biol. Reprod. 64: 1644-1652 [Abstract] [Full Text]
Putnam, A. J., Schultz, K., Mooney, D. J. (2001). Control of microtubule assembly by extracellular matrix and externally applied strain. Am. J. Physiol. Cell Physiol. 280: C556-C564 [Abstract] [Full Text]
Putnam, A., Cunningham, J., Dennis, R., Linderman, J., Mooney, D. (1998). Microtubule assembly is regulated by externally applied strain in cultured smooth muscle cells. J. Cell Sci. 111: 3379-3387 [Abstract]
Urrutia, R., Henley, J.�R., Cook, T., McNiven, M.�A. (1997). The dynamins: Redundant or distinct functions for an expanding family of�related�GTPases?. Proc. Natl. Acad. Sci. USA 94: 377-384 [Abstract] [Full Text]
Subtil, A, Dautry-Varsat, A (1997). Microtubule depolymerization inhibits clathrin coated-pit internalization in non-adherent cell lines while interleukin 2 endocytosis is not affected. J. Cell Sci. 110: 2441-2447 [Abstract]
Tagawa, H., Rozich, J. D., Tsutsui, H., Narishige, T., Kuppuswamy, D., Sato, H., McDermott, P. J., Koide, M., Cooper, G. IV (1996). Basis for Increased Microtubules in Pressure-Hypertrophied Cardiocytes. Circulation 93: 1230-1243 [Abstract] [Full Text]
Mooney, D., Langer, R, Ingber, D. (1995). Cytoskeletal filament assembly and the control of cell spreading and function by extracellular matrix. J. Cell Sci. 108: 2311-2320 [Abstract]
Robinson, J., Vandre, D. (1995). Stimulus-dependent alterations in macrophage microtubules: increased tubulin polymerization and detyrosination. J. Cell Sci. 108: 645-655 [Abstract]
Paturle-Lafanechere, L, Manier, M, Trigault, N, Pirollet, F, Mazarguil, H, Job, D (1994). Accumulation of delta 2-tubulin, a major tubulin variant that cannot be tyrosinated, in neuronal tissues and in stable microtubule assemblies. J. Cell Sci. 107: 1529-1543 [Abstract]
Harlow, P, Nemer, M (1987). Coordinate and selective beta-tubulin gene expression associated with cilium formation in sea urchin embryos.. Genes Dev. 1: 1293-1304 [Abstract]