Probing the structure of cytoplasm

doi:10.1083/jcb.102.6.2015

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Probing the structure of cytoplasm

K Luby-Phelps, DL Taylor and F Lanni

We have used size-fractionated, fluorescent dextrans to probe the structure of the cytoplasmic ground substance of living Swiss 3T3 cells by fluorescence recovery after photobleaching and video image processing. The data indicate that the cytoplasm of living cells has a fluid phase viscosity four times greater than water and contains structural barriers that restrict free diffusion of dissolved macromolecules in a size-dependent manner. Assuming these structural barriers comprise a filamentous meshwork, the combined fluorescence recovery after photobleaching and imaging data suggest that the average pore size of the meshwork is in the range of 300 to 400 A, but may be as small as 200 A in some cytoplasmic domains.
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Sykova, E., Nicholson, C. (2008). Diffusion in Brain Extracellular Space. Physiol. Rev. 88: 1277-1340 [Abstract] [Full Text]
Stork, T., Engelen, D., Krudewig, A., Silies, M., Bainton, R. J., Klambt, C. (2008). Organization and Function of the Blood Brain Barrier in Drosophila. J. Neurosci. 28: 587-597 [Abstract] [Full Text]
Dijksterhuis, J., Nijsse, J., Hoekstra, F. A., Golovina, E. A. (2007). High Viscosity and Anisotropy Characterize the Cytoplasm of Fungal Dormant Stress-Resistant Spores. Eukaryot Cell 6: 157-170 [Abstract] [Full Text]
Thorne, R. G., Nicholson, C. (2006). In vivo diffusion analysis with quantum dots and dextrans predicts the width of brain extracellular space. Proc. Natl. Acad. Sci. USA 103: 5567-5572 [Abstract] [Full Text]
Gregor, T., Bialek, W., van Steveninck, R. R. d. R., Tank, D. W., Wieschaus, E. F. (2005). Diffusion and scaling during early embryonic pattern formation. Proc. Natl. Acad. Sci. USA 102: 18403-18407 [Abstract] [Full Text]
Chang, P., Torres, J., Lewis, R. A., Mowry, K. L., Houliston, E., King, M. L. (2004). Localization of RNAs to the Mitochondrial Cloud in Xenopus Oocytes through Entrapment and Association with Endoplasmic Reticulum. Mol. Biol. Cell 15: 4669-4681 [Abstract] [Full Text]
Lacayo, C. I., Theriot, J. A. (2004). Listeria monocytogenes Actin-based Motility Varies Depending on Subcellular Location: A Kinematic Probe for Cytoarchitecture. Mol. Biol. Cell 15: 2164-2175 [Abstract] [Full Text]
Tseng, Y., Lee, J. S. H., Kole, T. P., Jiang, I., Wirtz, D. (2004). Micro-organization and visco-elasticity of the interphase nucleus revealed by particle nanotracking. J. Cell Sci. 117: 2159-2167 [Abstract] [Full Text]
Snapp, E. L., Reinhart, G. A., Bogert, B. A., Lippincott-Schwartz, J., Hegde, R. S. (2004). The organization of engaged and quiescent translocons in the endoplasmic reticulum of mammalian cells. JCB 164: 997-1007 [Abstract] [Full Text]
Ruchira, , Hink, M. A., Bosgraaf, L., van Haastert, P. J. M., Visser, A. J. W. G. (2004). Pleckstrin Homology Domain Diffusion in Dictyostelium Cytoplasm Studied Using Fluorescence Correlation Spectroscopy. J. Biol. Chem. 279: 10013-10019 [Abstract] [Full Text]
Larson, D. R., Ma, Y. M., Vogt, V. M., Webb, W. W. (2003). Direct measurement of Gag-Gag interaction during retrovirus assembly with FRET and fluorescence correlation spectroscopy. JCB 162: 1233-1244 [Abstract] [Full Text]
Rizzo, M. A., Magnuson, M. A., Drain, P. F., Piston, D. W. (2002). A Functional Link between Glucokinase Binding to Insulin Granules and Conformational Alterations in Response to Glucose and Insulin. J. Biol. Chem. 277: 34168-34175 [Abstract] [Full Text]
Petty, H. R., Kindzelskii, A. L. (2001). Dissipative metabolic patterns respond during neutrophil transmembrane signaling. Proc. Natl. Acad. Sci. USA 10.1073/pnas.061014298v1 [Abstract] [Full Text]
Terasaki, M., Campagnola, P., Rolls, M. M., Stein, P. A., Ellenberg, J., Hinkle, B., Slepchenko, B. (2001). A New Model for Nuclear Envelope Breakdown. Mol. Biol. Cell 12: 503-510 [Abstract] [Full Text]
Shafrir, Y, ben-Avraham, D, Forgacs, G (2000). Trafficking and signaling through the cytoskeleton: a specific mechanism. J. Cell Sci. 113: 2747-2757 [Abstract]
Han, W., Ng, Y.-K., Axelrod, D., Levitan, E. S. (1999). Neuropeptide release by efficient recruitment of diffusing cytoplasmic secretory vesicles. Proc. Natl. Acad. Sci. USA 96: 14577-14582 [Abstract] [Full Text]
Murphy, E. J. (1998). L-FABP and I-FABP expression increase NBD-stearate uptake and cytoplasmic diffusion in L cells. Am. J. Physiol. Gastrointest. Liver Physiol. 275: G244-G249 [Abstract] [Full Text]
Lamond, A. I., Earnshaw, W. C. (1998). Structure and Function in the Nucleus. Science 280: 547-553 [Abstract] [Full Text]
Luxon, B. A., Holly, D. C., Milliano, M. T., Weisiger, R. A. (1998). Sex differences in multiple steps in hepatic transport of palmitate support a balanced uptake mechanism. Am. J. Physiol. Gastrointest. Liver Physiol. 274: G52-G61 [Abstract] [Full Text]
Seksek, O., Biwersi, J., Verkman, A.S. (1997). Translational Diffusion of Macromolecule-sized Solutes in Cytoplasm and Nucleus. JCB 138: 131-142 [Abstract] [Full Text]
Terasaki, M. (1994). Redistribution of cytoplasmic components during germinal vesicle breakdown in starfish oocytes. J. Cell Sci. 107: 1797-1805 [Abstract]
Provance, D., McDowall, A, Marko, M, Luby-Phelps, K (1993). Cytoarchitecture of size-excluding compartments in living cells. J. Cell Sci. 106: 565-577 [Abstract]
Yam, K., Anderson, D., Buxbaum, R. (1988). Diffusion of small solutes in polymer-containing solutions. Science 241: 330-332 [Abstract]
Edgar, B A, Odell, G M, Schubiger, G (1987). Cytoarchitecture and the patterning of fushi tarazu expression in the Drosophila blastoderm.. Genes Dev. 1: 1226-1237 [Abstract]
Swanson, J., McNeil, P. (1987). Nuclear reassembly excludes large macromolecules. Science 238: 548-550 [Abstract]
Petty, H. R., Kindzelskii, A. L. (2001). Dissipative metabolic patterns respond during neutrophil transmembrane signaling. Proc. Natl. Acad. Sci. USA 98: 3145-3149 [Abstract] [Full Text]