The Journal of Cell Biology, Vol 102, 2273-2280, Copyright © 1986 by The Rockefeller University Press
Rapid adhesion of nerve cells to muscle fibers from adult rats is mediated by a sialic acid-binding receptor
R Bischoff
Single viable muscle fibers isolated from adult rats by collagenase
digestion rapidly bind dissociated spinal neurons or PC-12 cells but not a
variety of other cells tested. The adhesion process is calcium-
independent, temperature-sensitive, and is not blocked by pretreating cells
with inhibitors of energy metabolism or actin polymerization. Adhesion is
mediated by a carbohydrate-binding protein and can be inhibited by
N-acetylneuraminic acid or mucin, a glycoprotein with high sialic acids
content. The hapten inhibitors do not dissociate cells if added after
aggregation has occurred. Experiments to block adhesion by pretreatment of
cells with either neuraminidase or mucin show that the sialic acids-rich
moiety is on the nerve cells, while its receptor is on the muscle fibers.
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