Molecular cloning of cDNA for rat liver gap junction protein

doi:10.1083/jcb.103.1.123

Quantitative Colocalization Analysis Software

This Article

	Full Text (PDF, 1303K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Paul, D. L.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Paul, D. L.


Social Bookmarking

	What's this?

ARTICLES

Molecular cloning of cDNA for rat liver gap junction protein

DL Paul

An affinity-purified antibody directed against the 27-kD protein associated with isolated rat liver gap junctions was produced. Light and electron microscopic immunocytochemistry showed that this antigen was localized specifically to the cytoplasmic surfaces of gap junctions. The antibody was used to select cDNA from a rat liver library in the expression vector lambda gt11. The largest cDNA selected contained 1,494 bp and coded for a protein with a calculated molecular mass of 32,007 daltons. Northern blot analysis indicated that brain, kidney, and stomach express an mRNA with similar size and homology to that expressed in liver, but that heart and lens express differently sized, less homologous mRNA.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Hanstein, R., Trotter, J., Behl, C., Clement, A. B. (2009). Increased Connexin 43 Expression as a Potential Mediator of the Neuroprotective Activity of the Corticotropin-Releasing Hormone. Mol. Endocrinol. 23: 1479-1493 [Abstract] [Full Text]
Franke, W. W. (2009). Discovering the Molecular Components of Intercellular Junctions--A Historical View. Cold Spring Harb. Perspect. Biol. 1: a003061-a003061 [Abstract] [Full Text]
Beahm, D. L., Oshima, A., Gaietta, G. M., Hand, G. M., Smock, A. E., Zucker, S. N., Toloue, M. M., Chandrasekhar, A., Nicholson, B. J., Sosinsky, G. E. (2006). Mutation of a Conserved Threonine in the Third Transmembrane Helix of {alpha}- and beta-Connexins Creates a Dominant-negative Closed Gap Junction Channel. J. Biol. Chem. 281: 7994-8009 [Abstract] [Full Text]
Powell, K. (2006). Filling in the gap: cloning a connexin. JCB 172: 6-6 [Full Text]
Ek-Vitorin, J. F., Burt, J. M. (2005). Quantification of gap junction selectivity. Am. J. Physiol. Cell Physiol. 289: C1535-C1546 [Abstract] [Full Text]
Hokaiwado, N., Asamoto, M., Ogawa, K., Shirai, T. (2005). Transgenic Disruption of Gap Junctional Intercellular Communication Enhances Early but Not Late Stage Hepatocarcinogenesis in the Rat. Toxicol Pathol 33: 695-701 [Abstract] [Full Text]
Panchin, Y. V. (2005). Evolution of gap junction proteins - the pannexin alternative. J. Exp. Biol. 208: 1415-1419 [Abstract] [Full Text]
SAEZ, J. C., BERTHOUD, V. M., BRANES, M. C., MARTINEZ, A. D., BEYER, E. C. (2003). Plasma Membrane Channels Formed by Connexins: Their Regulation and Functions. Physiol. Rev. 83: 1359-1400 [Abstract] [Full Text]
Kronengold, J., Trexler, E.B., Bukauskas, F.F., Bargiello, T.A., Verselis, V.K. (2003). Single-channel SCAM Identifies Pore-lining Residues in the First Extracellular Loop and First Transmembrane Domains of Cx46 Hemichannels. JGP 122: 389-405 [Abstract] [Full Text]
Menichella, D. M., Goodenough, D. A., Sirkowski, E., Scherer, S. S., Paul, D. L. (2003). Connexins Are Critical for Normal Myelination in the CNS. J. Neurosci. 23: 5963-5973 [Abstract] [Full Text]
Caton, D., Calabrese, A., Mas, C., Serre-Beinier, V., Charollais, A., Caille, D., Zufferey, R., Trono, D., Meda, P. (2003). Lentivirus-mediated transduction of connexin cDNAs shows level- and isoform-specific alterations in insulin secretion of primary pancreatic {beta}-cells. J. Cell Sci. 116: 2285-2294 [Abstract] [Full Text]
Dufresne, J., Finnson, K. W., Gregory, M., Cyr, D. G. (2003). Expression of multiple connexins in the rat epididymis indicates a complex regulation of gap junctional communication. Am. J. Physiol. Cell Physiol. 284: C33-C43 [Abstract] [Full Text]
Skerrett, I.M., Aronowitz, J., Shin, J.H., Cymes, G., Kasperek, E., Cao, F.L., Nicholson, B.J. (2002). Identification of amino acid residues lining the pore of a gap junction channel. JCB 159: 349-360 [Abstract] [Full Text]
Altevogt, B. M., Kleopa, K. A., Postma, F. R., Scherer, S. S., Paul, D. L. (2002). Connexin29 Is Uniquely Distributed within Myelinating Glial Cells of the Central and Peripheral Nervous Systems. J. Neurosci. 22: 6458-6470 [Abstract] [Full Text]
Abrams, C. K., Bennett, M. V. L., Verselis, V. K., Bargiello, T. A. (2002). Voltage opens unopposed gap junction hemichannels formed by a connexin 32 mutant associated with X-linked Charcot-Marie-Tooth disease. Proc. Natl. Acad. Sci. USA 99: 3980-3984 [Abstract] [Full Text]
Muramatsu, A., Iwai, M., Morikawa, T., Tanaka, S., Mori, T., Harada, Y., Okanoue, T. (2002). Influence of transfection with connexin 26 gene on malignant potential of human hepatoma cells. Carcinogenesis 23: 351-358 [Abstract] [Full Text]
Oguro, K., Jover, T., Tanaka, H., Lin, Y., Kojima, T., Oguro, N., Grooms, S. Y., Bennett, M. V. L., Zukin, R. S. (2001). Global Ischemia-Induced Increases in the Gap Junctional Proteins Connexin 32 (Cx32) and Cx36 in Hippocampus and Enhanced Vulnerability of Cx32 Knock-Out Mice. J. Neurosci. 21: 7534-7542 [Abstract] [Full Text]
Alford, A. I., Rannels, D. E. (2001). Extracellular matrix fibronectin alters connexin43 expression by alveolar epithelial cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 280: L680-L688 [Abstract] [Full Text]
Berthoud, V. M., Beyer, E. C., Seul, K. H. (2000). Peptide inhibitors of intercellular communication. Am. J. Physiol. Lung Cell. Mol. Physiol. 279: L619-L622 [Full Text]
Alvarez-Maubecin, V., Garcia-Hernandez, F., Williams, J. T., Van Bockstaele, E. J. (2000). Functional Coupling between Neurons and Glia. J. Neurosci. 20: 4091-4098 [Abstract] [Full Text]
Chang, Q., Pereda, A., Pinter, M. J., Balice-Gordon, R. J. (2000). Nerve Injury Induces Gap Junctional Coupling among Axotomized Adult Motor Neurons. J. Neurosci. 20: 674-684 [Abstract] [Full Text]
Chang, Q., Gonzalez, M., Pinter, M. J., Balice-Gordon, R. J. (1999). Gap Junctional Coupling and Patterns of Connexin Expression among Neonatal Rat Lumbar Spinal Motor Neurons. J. Neurosci. 19: 10813-10828 [Abstract] [Full Text]
Grümmer, R., Traub, O., Winterhager, E. (1999). Gap Junction Connexin Genes cx26 and cx43 Are Differentially Regulated by Ovarian Steroid Hormones in Rat Endometrium. Endocrinology 140: 2509-2516 [Abstract] [Full Text]
Peracchia, C., Wang, X. G., Peracchia, L. L. (1999). Is the chemical gate of connexins voltage sensitive? Behavior of Cx32 wild-type and mutant channels. Am. J. Physiol. Cell Physiol. 276: C1361-C1373 [Abstract] [Full Text]
Theodorakis, N. G., de Maio, A. (1999). Cx32 mRNA in rat liver: effects of inflammation on poly(A) tail distribution and mRNA degradation. Am. J. Physiol. Regul. Integr. Comp. Physiol. 276: R1249-R1257 [Abstract] [Full Text]
Piechocki, M. P., Burk, R. D., Ruch, R. J. (1999). Regulation of connexin32 and connexin43 gene expression by DNA methylation in rat liver cells. Carcinogenesis 20: 401-406 [Abstract] [Full Text]
George, C. H., Kendall, J. M., Campbell, A. K., Evans, W. H. (1998). Connexin-Aequorin Chimerae Report Cytoplasmic Calcium Environments along Trafficking Pathways Leading to Gap Junction Biogenesis in Living COS-7 Cells. J. Biol. Chem. 273: 29822-29829 [Abstract] [Full Text]
Cotrina, M. L., Lin, J. H.-C., Nedergaard, M. (1998). Cytoskeletal Assembly and ATP Release Regulate Astrocytic Calcium Signaling. J. Neurosci. 18: 8794-8804 [Abstract] [Full Text]
Wang, X. G., Peracchia, C. (1998). Molecular dissection of a basic COOH-terminal domain of Cx32 that inhibits gap junction gating sensitivity. Am. J. Physiol. Cell Physiol. 275: C1384-C1390 [Abstract] [Full Text]
Guo, R., Liu, L., Barajas, L. (1998). RT-PCR study of the distribution of connexin 43�mRNA in the glomerulus and renal tubular segments. Am. J. Physiol. Regul. Integr. Comp. Physiol. 275: R439-R447 [Abstract] [Full Text]
Ressot, C., Gomes, D., Dautigny, A., Pham-Dinh, D., Bruzzone, R. (1998). Connexin32 Mutations Associated with X-Linked Charcot-Marie-Tooth Disease Show Two Distinct Behaviors: Loss of Function and Altered Gating Properties. J. Neurosci. 18: 4063-4075 [Abstract] [Full Text]
Foote, C. I., Zhou, L., Zhu, X., Nicholson, B. J. (1998). The Pattern of Disulfide Linkages in the Extracellular Loop Regions of Connexin 32 Suggests a Model for the Docking Interface of Gap Junctions. JCB 140: 1187-1197 [Abstract] [Full Text]
Martin, P. E.�M., George, C. H., Castro, C., Kendall, J. M., Capel, J., Campbell, A. K., Revilla, A., Barrio, L. C., Evans, W. H. (1998). Assembly of Chimeric Connexin-Aequorin Proteins into Functional Gap Junction Channels. REPORTING INTRACELLULAR AND PLASMA MEMBRANE CALCIUM ENVIRONMENTS. J. Biol. Chem. 273: 1719-1726 [Abstract] [Full Text]
Itahana, K., Tanaka, T., Morikazu, Y., Komatu, S., Ishida, N., Takeya, T. (1998). Isolation and Characterization of a Novel Connexin Gene, Cx-60, in Porcine Ovarian Follicles. Endocrinology 139: 320-329 [Abstract] [Full Text]
Cao, F, Eckert, R, Elfgang, C, Nitsche, J., Snyder, S., H-ulser, D., Willecke, K, Nicholson, B. (1998). A quantitative analysis of connexin-specific permeability differences of gap junctions expressed in HeLa transfectants and Xenopus oocytes. J. Cell Sci. 111: 31-43 [Abstract]
Oyoyo, U. A., Shah, U. S., Murray, S. A. (1997). The Role of {alpha}1 (Connexin-43) Gap Junction Expression in Adrenal Cortical Cell Function. Endocrinology 138: 5385-5397 [Abstract] [Full Text]
Deschenes, S. M., Walcott, J. L., Wexler, T. L., Scherer, S. S., Fischbeck, K. H. (1997). Altered Trafficking of Mutant Connexin32. J. Neurosci. 17: 9077-9084 [Abstract] [Full Text]
Statuto, M., Audebet, C., Tonoli, H., Selmi-Ruby, S., Rousset, B., Munari-Silem, Y. (1997). Restoration of Cell-to-Cell Communication in Thyroid Cell Lines by Transfection with and Stable Expression of the Connexin-32 Gene. IMPACT ON CELL PROLIFERATION AND TISSUE-SPECIFIC GENE EXPRESSION. J. Biol. Chem. 272: 24710-24716 [Abstract] [Full Text]
Nadarajah, B., Jones, A. M., Evans, W. H., Parnavelas, J. G. (1997). Differential Expression of Connexins during Neocortical Development and Neuronal Circuit Formation. J. Neurosci. 17: 3096-3111 [Abstract] [Full Text]
Wang, H.-Z., Veenstra, R. D. (1997). Monovalent Ion Selectivity Sequences of the Rat Connexin43 Gap Junction Channel. JGP 109: 491-507 [Abstract] [Full Text]
Wang, Y, Rose, B (1997). An inhibition of gap-junctional communication by cadherins. J. Cell Sci. 110: 301-309 [Abstract]
Ishimatsu, M., Williams, J. T. (1996). Synchronous Activity in Locus Coeruleus Results from Dendritic Interactions in Pericoerulear Regions. J. Neurosci. 16: 5196-5204 [Abstract] [Full Text]
Dahl, E., Manthey, D., Chen, Y., Schwarz, H.-J., Chang, Y. S., Lalley, P. A., Nicholson, B. J., Willecke, K. (1996). Molecular Cloning and Functional Expression of Mouse Connexin-30,a Gap Junction Gene Highly Expressed in Adult Brain and Skin. J. Biol. Chem. 271: 17903-17910 [Abstract] [Full Text]
Cascio, M., Kumar, N. M., Safarik, R., Gilula, N. B. (1995). Physical Characterization of Gap Junction Membrane Connexons (Hemi-channels) Isolated from Rat Liver. J. Biol. Chem. 270: 18643-18648 [Abstract] [Full Text]
Kumar, N., Friend, D., Gilula, N. (1995). Synthesis and assembly of human beta 1 gap junctions in BHK cells by DNA transfection with the human beta 1 cDNA. J. Cell Sci. 108: 3725-3734 [Abstract]
Wang, Y, Rose, B (1995). Clustering of Cx43 cell-to-cell channels into gap junction plaques: regulation by cAMP and microfilaments. J. Cell Sci. 108: 3501-3508 [Abstract]
Kojima, T, Mitaka, T, Shibata, Y, Mochizuki, Y (1995). Induction and regulation of connexin26 by glucagon in primary cultures of adult rat hepatocytes. J. Cell Sci. 108: 2771-2780 [Abstract]
Guerrier, A, Fonlupt, P, Morand, I, Rabilloud, R, Audebet, C, Krutovskikh, V, Gros, D, Rousset, B, Munari-Silem, Y (1995). Gap junctions and cell polarity: connexin32 and connexin43 expressed in polarized thyroid epithelial cells assemble into separate gap junctions, which are located in distinct regions of the lateral plasma membrane domain. J. Cell Sci. 108: 2609-2617 [Abstract]
Kojima, T, Mitaka, T, Paul, D., Mori, M, Mochizuki, Y (1995). Reappearance and long-term maintenance of connexin32 in proliferated adult rat hepatocytes: use of serum-free L-15 medium supplemented with EGF and DMSO. J. Cell Sci. 108: 1347-1357 [Abstract]
Becker, D., Evans, W., Green, C., Warner, A (1995). Functional analysis of amino acid sequences in connexin43 involved in intercellular communication through gap junctions. J. Cell Sci. 108: 1455-1467 [Abstract]
Leube, R. (1995). The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains. J. Cell Sci. 108: 883-894 [Abstract]
Paul, D., Yu, K, Bruzzone, R, Gimlich, R., Goodenough, D. (1995). Expression of a dominant negative inhibitor of intercellular communication in the early Xenopus embryo causes delamination and extrusion of cells. Development 121: 371-381 [Abstract]
Kojima, T, Sawada, N, Oyamada, M, Chiba, H, Isomura, H, Mori, M (1994). Rapid appearance of connexin 26-positive gap junctions in centrilobular hepatocytes without induction of mRNA and protein synthesis in isolated perfused liver of female rat. J. Cell Sci. 107: 3579-3590 [Abstract]
Bruzzone, R, White, T., Paul, D. (1994). Expression of chimeric connexins reveals new properties of the formation and gating behavior of gap junction channels. J. Cell Sci. 107: 955-967 [Abstract]
Neveu, M., Hully, J., Babcock, K., Hertzberg, E., Nicholson, B., Paul, D., Pitot, H. (1994). Multiple mechanisms are responsible for altered expression of gap junction genes during oncogenesis in rat liver. J. Cell Sci. 107: 83-95 [Abstract]
Bergoffen, J, Scherer, S., Wang, S, Scott, M., Bone, L., Paul, D., Chen, K, Lensch, M., Chance, P., Fischbeck, K. (1993). Connexin mutations in X-linked Charcot-Marie-Tooth disease. Science 262: 2039-2042 [Abstract]
Sainio, K, Gilbert, S., Lehtonen, E, Nishi, M, Kumar, N., Gilula, N., Saxen, L (1992). Differential expression of gap junction mRNAs and proteins in the developing murine kidney and in experimentally induced nephric mesenchymes. Development 115: 827-837 [Abstract]
Franke, W.W., Troyanovsky, S.M., Koch, P.J., Troyanovsky, R., Fouquet, B., Leube, R.E. (1992). Desmosomal Proteins: Mediators of Intercellular Coupling and Intermediate Filament Anchorage. Cold Spring Harb Symp Quant Biol 57: 643-652 [Abstract]
Albright, C. D., Jones, R. T., Grimley, P. M., Resau, J. H. (1990). Intercellular Communication in Bronchial Epithelial Cells: Review of Evidence for a Possible Role in Lung Carcinogenesis. Toxicol Pathol 18: 324-343 [Abstract]
Ebihara, L, Beyer, E., Swenson, K., Paul, D., Goodenough, D. (1989). Cloning and expression of a Xenopus embryonic gap junction protein. Science 243: 1194-1195 [Abstract]
Thomas, L, Hartung, K, Langosch, D, Rehm, H, Bamberg, E, Franke, W., Betz, H (1988). Identification of synaptophysin as a hexameric channel protein of the synaptic vesicle membrane. Science 242: 1050-1053 [Abstract]
Azarnia, R, Reddy, S, Kmiecik, T., Shalloway, D, Loewenstein, W. (1988). The cellular src gene product regulates junctional cell-to-cell communication. Science 239: 398-401 [Abstract]
Sudhof, T., Lottspeich, F, Greengard, P, Mehl, E, Jahn, R (1987). A synaptic vesicle protein with a novel cytoplasmic domain and four transmembrane regions. Science 238: 1142-1144 [Abstract]
Dahl, G, Miller, T, Paul, D, Voellmy, R, Werner, R (1987). Expression of functional cell-cell channels from cloned rat liver gap junction complementary DNA. Science 236: 1290-1293 [Abstract]