Evidence for posttranscriptional regulation of the keratins expressed during hyperproliferation and malignant transformation in human epidermis

doi:10.1083/jcb.103.5.1945

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Evidence for posttranscriptional regulation of the keratins expressed during hyperproliferation and malignant transformation in human epidermis

AL Tyner and E Fuchs

Keratin K6 is a protein that is expressed in human skin under conditions of hyperproliferation (e.g., wound-healing, psoriasis, and cell culture) and malignant transformation (e.g., squamous cell carcinomas). When induced, the appearance of K6 is rapid: if skin tissue is placed in radiolabeled culture medium, this protein can be detected within an hour. The regulation of K6 seems to be controlled partly by a posttranscriptional mechanism: At least two K6 genes are actively transcribed both in vivo, when the protein is not made, as well as in vitro, when abundant levels of the protein are expressed. Substantial levels of K6a and K6b RNAs can be detected in skin by Northern Blot analysis, and these RNAs are largely, if not fully translatable in vitro. In situ hybridizations reveal that the RNAs are distributed throughout the living layers of the epidermis. The rapid induction of K6 expression through a posttranscriptional regulatory mechanism suggests that this keratin may play an important role in designing a cytoskeletal architecture that is compatible with the hyperproliferative state.
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Takahashi, K., Coulombe, P. A. (1997). Defining a Region of the Human Keratin 6a Gene That Confers Inducible Expression in Stratified Epithelia of Transgenic Mice. J. Biol. Chem. 272: 11979-11985 [Abstract] [Full Text]
Reichelt, J, Bauer, C, Porter, R, Lane, E, Magin, V (1997). Out of balance: consequences of a partial keratin 10 knockout. J. Cell Sci. 110: 2175-2186 [Abstract]
Takahashi, K., Paladini, R. D., Coulombe, P. A. (1995). Cloning and Characterization of Multiple Human Genes and cDNAs Encoding Highly Related Type II Keratin 6 Isoforms. J. Biol. Chem. 270: 18581-18592 [Abstract] [Full Text]
Chan, Y, Anton-Lamprecht, I, Yu, Q C, Jackel, A, Zabel, B, Ernst, J P, Fuchs, E (1994). A human keratin 14 "knockout": the absence of K14 leads to severe epidermolysis bullosa simplex and a function for an intermediate filament protein.. Genes Dev. 8: 2574-2587 [Abstract]
Byrne, C., Tainsky, M., Fuchs, E. (1994). Programming gene expression in developing epidermis. Development 120: 2369-2383 [Abstract]
Arnemann, J, Sullivan, K., Magee, A., King, I., Buxton, R. (1993). Stratification-related expression of isoforms of the desmosomal cadherins in human epidermis. J. Cell Sci. 104: 741-750 [Abstract]
Vassar, R, Fuchs, E (1991). Transgenic mice provide new insights into the role of TGF-alpha during epidermal development and differentiation.. Genes Dev. 5: 714-727 [Abstract]
Dale, B. A., Salonen, J., Jones, A. H. (1990). New Approaches And Concepts in The Study of Differentiation of Oral Epithelia. CROBM 1: 167-190 [Abstract] [Full Text]
Kopan, R, Fuchs, E (1989). A new look into an old problem: keratins as tools to investigate determination, morphogenesis, and differentiation in skin.. Genes Dev. 3: 1-15 [Abstract]