Acetylcholine receptor clustering and nuclear movement in muscle fibers in culture

doi:10.1083/jcb.104.1.87

This Article

	Full Text (PDF, 2279K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Englander, L. L.
	Articles by Rubin, L. L.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Englander, L. L.
	Articles by Rubin, L. L.


Social Bookmarking

	What's this?

ARTICLES

Acetylcholine receptor clustering and nuclear movement in muscle fibers in culture

LL Englander and LL Rubin

We have studied the formation of acetylcholine receptor (AChR) clusters and the behavior of myonuclei in rat and chick skeletal muscle cells grown in cell culture. These cells were treated with a factor derived from Torpedo electric extracellular matrix, which causes a large increase in their number of AChR clusters. We found that these clusters were located preferentially in membrane regions above myonuclei. This cluster-nucleus colocalization is explained by our finding that most of the nuclei near clusters remain relatively stationary, while most of those away from clusters are able to translocate throughout the myotube. In some cases, clusters clearly formed first, then nuclei migrated underneath and became immobilized. If clustered AChRs later dispersed, their associated nuclei resumed moving. These results suggest that AChR clustering initiates an extensive cytoskeletal rearrangement that causes the subcluster localization of organelles, potentially providing a stable source of newly synthesized AChRs for insertion into the cluster.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Carvalho, L., Stuhmer, J., Bois, J. S., Kalaidzidis, Y., Lecaudey, V., Heisenberg, C.-P. (2009). Control of convergent yolk syncytial layer nuclear movement in zebrafish. Development 136: 1305-1315 [Abstract] [Full Text]
Henriquez, J. P., Webb, A., Bence, M., Bildsoe, H., Sahores, M., Hughes, S. M., Salinas, P. C. (2008). Wnt signaling promotes AChR aggregation at the neuromuscular synapse in collaboration with agrin. Proc. Natl. Acad. Sci. USA 105: 18812-18817 [Abstract] [Full Text]
Levy, J. R., Holzbaur, E. L. F. (2008). Dynein drives nuclear rotation during forward progression of motile fibroblasts. J. Cell Sci. 121: 3187-3195 [Abstract] [Full Text]
Ji, J. Y., Lee, R. T., Vergnes, L., Fong, L. G., Stewart, C. L., Reue, K., Young, S. G., Zhang, Q., Shanahan, C. M., Lammerding, J. (2007). Cell Nuclei Spin in the Absence of Lamin B1. J. Biol. Chem. 282: 20015-20026 [Abstract] [Full Text]
Zhang, X., Xu, R., Zhu, B., Yang, X., Ding, X., Duan, S., Xu, T., Zhuang, Y., Han, M. (2007). Syne-1 and Syne-2 play crucial roles in myonuclear anchorage and motor neuron innervation. Development 134: 901-908 [Abstract] [Full Text]
Bartolini, F., Gundersen, G. G. (2006). Generation of noncentrosomal microtubule arrays. J. Cell Sci. 119: 4155-4163 [Abstract] [Full Text]
Grady, R. M., Starr, D. A., Ackerman, G. L., Sanes, J. R., Han, M. (2005). From the Cover: Syne proteins anchor muscle nuclei at the neuromuscular junction. Proc. Natl. Acad. Sci. USA 102: 4359-4364 [Abstract] [Full Text]
Bruusgaard, J C, Liestol, K, Ekmark, M, Kollstad, K, Gundersen, K (2003). Number and spatial distribution of nuclei in the muscle fibres of normal mice studied in vivo. J. Physiol. 551: 467-478 [Abstract] [Full Text]
Chytilova, E., Macas, J., Sliwinska, E., Rafelski, S. M., Lambert, G. M., Galbraith, D. W. (2000). Nuclear Dynamics in Arabidopsis thaliana. Mol. Biol. Cell 11: 2733-2741 [Abstract] [Full Text]
Reinsch, S, Gonczy, P (1998). Mechanisms of nuclear positioning. J. Cell Sci. 111: 2283-2295 [Abstract]
Liou, J.-C., Fu, W.-M. (1997). Regulation of Quantal Secretion from Developing Motoneurons by Postsynaptic Activity-Dependent Release of NT-3. J. Neurosci. 17: 2459-2468 [Abstract] [Full Text]
Xiang, X., Osmani, A.H., Osmani, S.A., Roghi, C.H., Willins, D.A., Beckwith, S., Goldman, G., Chiu, Y., Xin, M., Liu, B., Morris, N.R. (1995). Analysis of Nuclear Migration in Aspergillus nidulans. Cold Spring Harb Symp Quant Biol 60: 813-819 [Abstract]
Ralston, E, Hall, Z. (1989). Transfer of a protein encoded by a single nucleus to nearby nuclei in multinucleated myotubes. Science 244: 1066-1069 [Abstract]
Apel, E. D., Lewis, R. M., Grady, R. M., Sanes, J. R. (2000). Syne-1, A Dystrophin- and Klarsicht-related Protein Associated with Synaptic Nuclei at the Neuromuscular Junction. J. Biol. Chem. 275: 31986-31995 [Abstract] [Full Text]