Microtubules containing acetylated alpha-tubulin in mammalian cells in culture

doi:10.1083/jcb.104.2.289

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Microtubules containing acetylated alpha-tubulin in mammalian cells in culture

G Piperno, M LeDizet and XJ Chang

The subcellular distribution of microtubules containing acetylated alpha-tubulin in mammalian cells in culture was analyzed with 6-11B-1, a monoclonal antibody specific for acetylated alpha-tubulin. Cultures of 3T3, HeLa, and PtK2 cells were grown on coverslips and observed by immunofluorescence microscopy after double-staining by 6-11B-1 and B-5- 1-2, a monoclonal antibody specific for all alpha-tubulins. The antibody 6-11B-1 binds to primary cilia, centrioles, mitotic spindles, midbodies, and to subsets of cytoplasmic microtubules in 3T3 and HeLa cells, but not in PtK2 cells. These observations confirm that the acetylation of alpha-tubulin is a modification occurring in different microtubule structures and in a variety of eukaryotic cells. Some features of the acetylation of cytoplasmic microtubules of mammalian cells are also described here. First, acetylated alpha-tubulin is present in microtubules that, under depolymerizing conditions, are more stable than the majority of cytoplasmic microtubules. In addition to the specific microtubule frameworks already mentioned, cytoplasmic microtubules resistant to nocodazole or colchicine, but not cold- resistant microtubules, contain more acetylated alpha-tubulin than the rest of cellular microtubules. Second, the alpha-tubulin in all cytoplasmic microtubules of 3T3 and HeLa cells becomes acetylated in the presence of taxol, a drug that stabilizes microtubules. Third, acetylation and deacetylation of cytoplasmic microtubules are reversible in cells released from exposure to 0 degrees C or antimitotic drugs. Fourth, the epitope recognized by the antibody 6-11B- 1 is not absolutely necessary for cell growth and division. This epitope is absent in PtK2 cells. The acetylation of alpha-tubulin could regulate the presence of microtubules in specific intracellular spaces by selective stabilization.
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Warren, J. C., Rutkowski, A., Cassimeris, L. (2006). Infection with Replication-deficient Adenovirus Induces Changes in the Dynamic Instability of Host Cell Microtubules. Mol. Biol. Cell 17: 3557-3568 [Abstract] [Full Text]
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Jouvenet, N., Wileman, T. (2005). African swine fever virus infection disrupts centrosome assembly and function. J. Gen. Virol. 86: 589-594 [Abstract] [Full Text]
Pyka, J., Glogowska, A., Dralle, H., Hoang-Vu, C., Klonisch, T. (2005). Cytoplasmic Domain of proEGF Affects Distribution and Post-Translational Modification of Microtubuli and Increases Microtubule-Associated Proteins 1b and 2 Production in Human Thyroid Carcinoma Cells. Cancer Res. 65: 1343-1351 [Abstract] [Full Text]
Naranatt, P. P., Krishnan, H. H., Smith, M. S., Chandran, B. (2005). Kaposi's Sarcoma-Associated Herpesvirus Modulates Microtubule Dynamics via RhoA-GTP-Diaphanous 2 Signaling and Utilizes the Dynein Motors To Deliver Its DNA to the Nucleus. J. Virol. 79: 1191-1206 [Abstract] [Full Text]
BIRUKOVA, A. A., BIRUKOV, K. G., SMUROVA, K., ADYSHEV, D., KAIBUCHI, K., ALIEVA, I., GARCIA, J. G. N., VERIN, A. D. (2004). Novel role of microtubules in thrombin-induced endothelial barrier dysfunction. FASEB J. 18: 1879-1890 [Abstract] [Full Text]
Steindler, C., Li, Z., Algarte, M., Alcover, A., Libri, V., Ragimbeau, J., Pellegrini, S. (2004). Jamip1 (Marlin-1) Defines a Family of Proteins Interacting with Janus Kinases and Microtubules. J. Biol. Chem. 279: 43168-43177 [Abstract] [Full Text]
Kim, K.-t., Ongusaha, P. P., Hong, Y.-K., Kurdistani, S. K., Nakamura, M., Lu, K. P., Lee, S. W. (2004). Function of Drg1/Rit42 in p53-dependent Mitotic Spindle Checkpoint. J. Biol. Chem. 279: 38597-38602 [Abstract] [Full Text]
Bouquet, C., Soares, S., von Boxberg, Y., Ravaille-Veron, M., Propst, F., Nothias, F. (2004). Microtubule-Associated Protein 1B Controls Directionality of Growth Cone Migration and Axonal Branching in Regeneration of Adult Dorsal Root Ganglia Neurons. J. Neurosci. 24: 7204-7213 [Abstract] [Full Text]
Cohen, T., Yao, T.-P. (2004). AcK-knowledge Reversible Acetylation. Sci Signal 2004: pe42-pe42 [Abstract] [Full Text]
Jouvenet, N., Monaghan, P., Way, M., Wileman, T. (2004). Transport of African Swine Fever Virus from Assembly Sites to the Plasma Membrane Is Dependent on Microtubules and Conventional Kinesin. J. Virol. 78: 7990-8001 [Abstract] [Full Text]
Nurnberger, J., Kribben, A., Saez, A. O., Heusch, G., Philipp, T., Phillips, C. L. (2004). The Invs Gene Encodes a Microtubule-Associated Protein. J. Am. Soc. Nephrol. 15: 1700-1710 [Abstract] [Full Text]
Iomini, C., Tejada, K., Mo, W., Vaananen, H., Piperno, G. (2004). Primary cilia of human endothelial cells disassemble under laminar shear stress. JCB 164: 811-817 [Abstract] [Full Text]
Qin, H., Diener, D. R., Geimer, S., Cole, D. G., Rosenbaum, J. L. (2004). Intraflagellar transport (IFT) cargo: IFT transports flagellar precursors to the tip and turnover products to the cell body. JCB 164: 255-266 [Abstract] [Full Text]
Liu, W., Xu, S., Woda, C., Kim, P., Weinbaum, S., Satlin, L. M. (2003). Effect of flow and stretch on the [Ca2+]i response of principal and intercalated cells in cortical collecting duct. Am. J. Physiol. Renal Physiol. 285: F998-F1012 [Abstract] [Full Text]
Haggarty, S. J., Koeller, K. M., Wong, J. C., Grozinger, C. M., Schreiber, S. L. (2003). Domain-selective small-molecule inhibitor of histone deacetylase 6 (HDAC6)-mediated tubulin deacetylation. Proc. Natl. Acad. Sci. USA 100: 4389-4394 [Abstract] [Full Text]
Pfannenschmid, F., Wimmer, V. C., Rios, R.-M., Geimer, S., Krockel, U., Leiherer, A., Haller, K., Nemcova, Y., Mages, W. (2003). Chlamydomonas DIP13 and human NA14: a new class of proteins associated with microtubule structures is involved in cell division. J. Cell Sci. 116: 1449-1462 [Abstract] [Full Text]
Fath, T., Eidenmuller, J., Brandt, R. (2002). Tau-Mediated Cytotoxicity in a Pseudohyperphosphorylation Model of Alzheimer's Disease. J. Neurosci. 22: 9733-9741 [Abstract] [Full Text]
Chang, W., Webster, D. R., Salam, A. A., Gruber, D., Prasad, A., Eiserich, J. P., Bulinski, J. C. (2002). Alteration of the C-terminal Amino Acid of Tubulin Specifically Inhibits Myogenic Differentiation. J. Biol. Chem. 277: 30690-30698 [Abstract] [Full Text]
Rapoport, M., Dawson, H. N., Binder, L. I., Vitek, M. P., Ferreira, A. (2002). Tau is essential to beta -amyloid-induced neurotoxicity. Proc. Natl. Acad. Sci. USA 99: 6364-6369 [Abstract] [Full Text]
Parker, J. S. L., Broering, T. J., Kim, J., Higgins, D. E., Nibert, M. L. (2002). Reovirus Core Protein {micro}2 Determines the Filamentous Morphology of Viral Inclusion Bodies by Interacting with and Stabilizing Microtubules. J. Virol. 76: 4483-4496 [Abstract] [Full Text]
Guardiola, A. R., Yao, T.-P. (2002). Molecular Cloning and Characterization of a Novel Histone Deacetylase HDAC10. J. Biol. Chem. 277: 3350-3356 [Abstract] [Full Text]
Correa, L. M., Miller, M. G. (2001). Microtubule Depolymerization in Rat Seminiferous Epithelium Is Associated with Diminished Tyrosination of {{alpha}}-Tubulin. Biol. Reprod. 64: 1644-1652 [Abstract] [Full Text]
Iomini, C., Babaev-Khaimov, V., Sassaroli, M., Piperno, G. (2001). Protein Particles in Chlamydomonas Flagella Undergo a Transport Cycle Consisting of Four Phases. JCB 153: 13-24 [Abstract] [Full Text]
Karbowski, M, Spodnik, J., Teranishi, M, Wozniak, M, Nishizawa, Y, Usukura, J, Wakabayashi, T (2001). Opposite effects of microtubule-stabilizing and microtubule-destabilizing drugs on biogenesis of mitochondria in mammalian cells. J. Cell Sci. 114: 281-291 [Abstract]
Pisano, C., Battistoni, A., Antoccia, A., Degrassi, F., Tanzarella, C. (2000). Changes in microtubule organization after exposure to a benzimidazole derivative in Chinese hamster cells. Mutagenesis 15: 507-515 [Abstract] [Full Text]
Horesh, D., Sapir, T., Francis, F., Wolf, S. G., Caspi, M., Elbaum, M., Chelly, J., Reiner, O. (1999). Doublecortin, a stabilizer of microtubules. Hum Mol Genet 8: 1599-1610 [Abstract] [Full Text]
Gonzalez-Garay, M. L., Chang, L., Blade, K., Menick, D. R., Cabral, F. (1999). A beta -Tubulin Leucine Cluster Involved in Microtubule Assembly and Paclitaxel Resistance. J. Biol. Chem. 274: 23875-23882 [Abstract] [Full Text]
Elliott, G., O'Hare, P. (1999). Live-Cell Analysis of a Green Fluorescent Protein-Tagged Herpes Simplex Virus Infection. J. Virol. 73: 4110-4119 [Abstract] [Full Text]
Narishige, T., Blade, K. L., Ishibashi, Y., Nagai, T., Hamawaki, M., Menick, D. R., Kuppuswamy, D., Cooper, G. IV (1999). Cardiac Hypertrophic and Developmental Regulation of the beta -Tubulin Multigene Family. J. Biol. Chem. 274: 9692-9697 [Abstract] [Full Text]
Goold, R., Owen, R, Gordon-Weeks, P. (1999). Glycogen synthase kinase 3beta phosphorylation of microtubule-associated protein 1B regulates the stability of microtubules in growth cones. J. Cell Sci. 112: 3373-3384 [Abstract]
Fukushige, T, Siddiqui, Z., Chou, M, Culotti, J., Gogonea, C., Siddiqui, S., Hamelin, M (1999). MEC-12, an alpha-tubulin required for touch sensitivity in C. elegans. J. Cell Sci. 112: 395-403 [Abstract]
Sonee, M., Barron, E., Yarber, F. A., Hamm-Alvarez, S. F. (1998). Taxol inhibits endosomal-lysosomal membrane trafficking at two distinct steps in CV-1 cells. Am. J. Physiol. Cell Physiol. 275: C1630-C1639 [Abstract] [Full Text]
Bré, M.-H., Redeker, V., Vinh, J., Rossier, J., Levilliers, N. (1998). Tubulin Polyglycylation: Differential Posttranslational Modification of Dynamic Cytoplasmic and Stable Axonemal Microtubules in Paramecium. Mol. Biol. Cell 9: 2655-2665 [Abstract] [Full Text]
Elliott, G., O'Hare, P. (1998). Herpes Simplex Virus Type 1�Tegument Protein VP22 Induces the Stabilization and Hyperacetylation of Microtubules. J. Virol. 72: 6448-6455 [Abstract] [Full Text]
Pazour, G. J., Wilkerson, C. G., Witman, G. B. (1998). A Dynein Light Chain Is Essential for the Retrograde Particle Movement of Intraflagellar Transport (IFT). JCB 141: 979-992 [Abstract] [Full Text]
Cook, T. A., Nagasaki, T., Gundersen, G. G. (1998). Rho Guanosine Triphosphatase Mediates the Selective Stabilization of Microtubules Induced by Lysophosphatidic Acid. JCB 141: 175-185 [Abstract] [Full Text]
Mills, J., Lee, V., Pittman, R. (1998). Activation of a PP2A-like phosphatase and dephosphorylation of tau protein characterize onset of the execution phase of apoptosis. J. Cell Sci. 111: 625-636 [Abstract]
Kato, M. V., Sato, H., Nagayoshi, M., Ikawa, Y. (1997). Upregulation of the Elongation Factor-1alpha Gene by p53 in Association With Death of an Erythroleukemic Cell Line. Blood 90: 1373-1378 [Abstract] [Full Text]
Merrick, S. E., Trojanowski, J. Q., Lee, V. M.-Y. (1997). Selective Destruction of Stable Microtubules and Axons by Inhibitors of Protein Serine/Threonine Phosphatases in Cultured Human Neurons (NT2N Cells). J. Neurosci. 17: 5726-5737 [Abstract] [Full Text]
Hai, B., Gorovsky, M.�A. (1997). Germ-line knockout heterokaryons of an essential alpha -tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in Tetrahymena�thermophila. Proc. Natl. Acad. Sci. USA 94: 1310-1315 [Abstract] [Full Text]
Mayer, A, Ivanov, I., Gravotta, D, Adesnik, M, Sabatini, D. (1996). Cell-free reconstitution of the transport of viral glycoproteins from the TGN to the basolateral plasma membrane of MDCK cells. J. Cell Sci. 109: 1667-1676 [Abstract]
Wilson, P., Forer, A, Leggiadro, C (1994). Evidence that kinetochore microtubules in crane-fly spermatocytes disassemble during anaphase primarily at the poleward end. J. Cell Sci. 107: 3015-3027 [Abstract]
Bhattacharyya, A, Brackenbury, R, Ratner, N (1994). Axons arrest the migration of Schwann cell precursors. Development 120: 1411-1420 [Abstract]
Baas, P., Pienkowski, T., Cimbalnik, K., Toyama, K, Bakalis, S, Ahmad, F., Kosik, K. (1994). Tau confers drug stability but not cold stability to microtubules in living cells. J. Cell Sci. 107: 135-143 [Abstract]
James, S., Silflow, C., Stroom, P, Lefebvre, P. (1993). A mutation in the alpha 1-tubulin gene of Chlamydomonas reinhardtii confers resistance to anti-microtubule herbicides. J. Cell Sci. 106: 209-218 [Abstract]
Muresan, V, Joshi, H., Besharse, J. (1993). Gamma-tubulin in differentiated cell types: localization in the vicinity of basal bodies in retinal photoreceptors and ciliated epithelia. J. Cell Sci. 104: 1229-1237 [Abstract]
Takemura, R., Okabe, S., Umeyama, T., Kanai, Y., Cowan, N. J., Hirokawa, N. (1992). Increased microtubule stability and alpha tubulin acetylation in cells transfected with microtubule-associated proteins MAP1B, MAP2 or tau. J. Cell Sci. 103: 953-964 [Abstract]
Ojakian, G. K., Schwimmer, R. (1992). Antimicrotubule drugs inhibit the polarized insertion of an intracellular glycoprotein pool into the apical membrane of Madin-Darby canine kidney (MDCK) cells. J. Cell Sci. 103: 677-687 [Abstract]
Scott, C. A., Walker, C. C., Neal, D. A., Harper, C. E., Bloodgood, R. A., Somers, K. D., Mills, S. E., Rebhun, L. I., Levine, P. A. (1990). {beta}-Tubulin Epitope Expression in Normal and Malignant Epithelial Cells. Arch Otolaryngol Head Neck Surg 116: 583-589 [Abstract]
Rapoport, M., Dawson, H. N., Binder, L. I., Vitek, M. P., Ferreira, A. (2002). Tau is essential to beta -amyloid-induced neurotoxicity. Proc. Natl. Acad. Sci. USA 99: 6364-6369 [Abstract] [Full Text]