Monoclonal antibodies in the analysis of fibronectin isoforms generated by alternative splicing of mRNA precursors in normal and transformed human cells

doi:10.1083/jcb.104.3.595

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Monoclonal antibodies in the analysis of fibronectin isoforms generated by alternative splicing of mRNA precursors in normal and transformed human cells

L Borsi, B Carnemolla, P Castellani, C Rosellini, D Vecchio, G Allemanni, SE Chang, J Taylor-Papadimitriou, H Pande and L Zardi

Recent results showing that a single fibronectin gene can give rise to several different mRNAs by alternative splicing have offered an explanation for fibronectin polymorphism. Here we report on monoclonal antibodies that show specificity for a fibronectin segment (ED) that can be included or omitted from the molecule depending on the pattern of splicing of the mRNA precursors. Using these monoclonals, we have quantitatively analyzed the expression of the ED sequence in human fibronectin from different sources. The results demonstrated that, at the protein level, the ED segment is not expressed in plasma fibronectin and that, in fibronectin from the tissue culture medium of tumor-derived or simian virus-40-transformed human cells, the percentage of fibronectin molecules containing the ED segment is about 10 times higher than in fibronectin from normal human fibroblasts. These results suggest that in malignant cells the mechanisms that regulate the splicing of mRNA precursors are altered.
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Zuk, A., Bonventre, J. V., Matlin, K. S. (2001). Expression of fibronectin splice variants in the postischemic rat kidney. Am. J. Physiol. Renal Physiol. 280: F1037-F1053 [Abstract] [Full Text]
Bochaton-Piallat, M.-L., Kapetanios, A. D., Donati, G., Redard, M., Gabbiani, G., Pournaras, C. J. (2000). TGF-{beta}1, TGF-{beta} Receptor II and ED-A Fibronectin Expression in Myofibroblast of Vitreoretinopathy. IOVS 41: 2336-2342 [Abstract] [Full Text]
Midulla, M., Verma, R., Pignatelli, M., Ritter, M. A., Courtenay-Luck, N. S., George, A. J. T. (2000). Source of Oncofetal ED-B-containing Fibronectin: Implications of Production by Both Tumor and Endothelial Cells. Cancer Res. 60: 164-169 [Abstract] [Full Text]
Faggin, E., Puato, M., Chiavegato, A., Franch, R., Pauletto, P., Sartore, S. (2000). Fish Oil Supplementation Prevents Neointima Formation in Nonhypercholesterolemic Balloon-Injured Rabbit Carotid Artery by Reducing Medial and Adventitial Cell Activation. Arterioscler. Thromb. Vasc. Bio. 20: 152-163 [Abstract] [Full Text]
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Moyano, J. V., Carnemolla, B., Albar, J. P., Leprini, A., Gaggero, B., Zardi, L., Garcia-Pardo, A. (1999). Cooperative Role for Activated alpha 4beta 1 Integrin and Chondroitin Sulfate Proteoglycans in Cell Adhesion to the Heparin III Domain of Fibronectin. IDENTIFICATION OF A NOVEL HEPARIN AND CELL BINDING SEQUENCE IN REPEAT III5. J. Biol. Chem. 274: 135-142 [Abstract] [Full Text]
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Bezie, Y., Lamaziere, J.-M. D., Laurent, S., Challande, P., Cunha, R. S., Bonnet, J., Lacolley, P. (1998). Fibronectin Expression and Aortic Wall Elastic Modulus in Spontaneously Hypertensive Rats. Arterioscler. Thromb. Vasc. Bio. 18: 1027-1034 [Abstract] [Full Text]
Manabe, R.-i., Oh-e, N., Maeda, T., Fukuda, T., Sekiguchi, K. (1997). Modulation of Cell-adhesive Activity of Fibronectin by the Alternatively Spliced EDA Segment. JCB 139: 295-307 [Abstract] [Full Text]
Moyano, J. V., Carnemolla, B., Dominguez-Jimenez, C., Garcia-Gila, M., Albar, J. P., Sanchez-Aparicio, P., Leprini, A., Querze, G., Zardi, L., Garcia-Pardo, A. (1997). Fibronectin Type III5 Repeat Contains a Novel Cell Adhesion Sequence, KLDAPT, Which Binds Activated alpha 4beta 1 and alpha 4beta 7 Integrins. J. Biol. Chem. 272: 24832-24836 [Abstract] [Full Text]
Slomp, J., Gittenberger-de Groot, A. C., Glukhova, M. A., van Munsteren, J. C., Kockx, M. M., Schwartz, S. M., Koteliansky, V. E. (1997). Differentiation, Dedifferentiation, and Apoptosis of Smooth Muscle Cells During the Development of the Human Ductus Arteriosus. Arterioscler. Thromb. Vasc. Bio. 17: 1003-1009 [Abstract] [Full Text]
Colucci, S, Giannelli, G, Grano, M, Faccio, R, Quaranta, V, Zallone, A. (1996). Human osteoclast-like cells selectively recognize laminin isoforms, an event that induces migration and activates Ca2+ mediated signals. J. Cell Sci. 109: 1527-1535 [Abstract]
Dubin, D., Peters, J. H., Brown, L. F., Logan, B., Kent, K. C., Berse, B., Berven, S., Cercek, B., Sharifi, B. G., Pratt, R. E., Dzau, V. J., Van De Water, L. (1995). Balloon Catheterization Induces Arterial Expression of Embryonic Fibronectins. Arterioscler. Thromb. Vasc. Bio. 15: 1958-1967 [Abstract] [Full Text]
Lukinmaa, P.-L., Vaheri, A. (1994). ED-A Region-containing Isoform of Cellular Fibronectin is Present in Dentin Matrix in Dentinogenesis Imperfecta Associated with Osteogenesis Imperfecta. JDR 73: 1187-1196 [Abstract]
Mardon, H., Grant, R., Grant, K., Harris, H (1993). Fibronectin splice variants are differentially incorporated into the extracellular matrix of tumorigenic and non-tumorigenic hybrids between normal fibroblasts and sarcoma cells. J. Cell Sci. 104: 783-792 [Abstract]
Mardon, H. J., Sebastio, G. (1992). Regulation of alternative splicing in the IIICS region of human fibronectin pre-mRNA encoding cell binding sites CS1 and CS5. J. Cell Sci. 103: 423-433 [Abstract]