100-kD coated vesicle proteins: molecular heterogeneity and intracellular distribution studied with monoclonal antibodies

doi:10.1083/jcb.104.4.887

Quantitative Colocalization Analysis Software

This Article

	Full Text (PDF, 3171K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Robinson, M. S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Robinson, M. S.


Social Bookmarking

	What's this?

ARTICLES

100-kD coated vesicle proteins: molecular heterogeneity and intracellular distribution studied with monoclonal antibodies

MS Robinson

Proteins with molecular weights of around 100,000 (designated 100K) are found in all coated vesicles. Five monoclonal antibodies have been raised against the major 100K proteins of bovine brain coated vesicles, which migrate on SDS gels as three closely spaced bands. One antibody stains the middle band (band B), two stain both upper and lower bands (bands A and C), and two stain the lower band (band C) only. Thus, the polypeptides in bands A and C are related (but not identical), a result confirmed by NH2-terminal sequencing. Other tissues were found to express proteins corresponding to, and co-migrating with, bands B and C but not band A. Only the two antibodies that recognize both A and C stained fixed and permeabilized tissue culture cells; they both showed a punctate pattern in the plane of the plasma membrane. Double labeling with anti-clathrin antibodies confirmed that the dots correspond to coated pits and vesicles. However, perinuclear staining seen with anti- clathrin, corresponding to Golgi-derived coated vesicles, was conspicuously absent with the two monoclonal antibodies. Affinity- purified polyclonal antisera against the 100K proteins, reported earlier, gave perinuclear as well as punctate staining; these included one antiserum which gave mainly perinuclear staining (Robinson, M. S., and B. M. F. Pearse, 1986, J. Cell Biol., 102:48-54). Thus, different 100K proteins appear to be found in different membrane compartments. Since the 100K proteins are thought to lie between clathrin and the membrane proteins of the vesicle, these results may help to explain how different membrane proteins can be sorted into coated vesicles in different parts of the cell.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Lau, A. W., Chou, M. M. (2008). The adaptor complex AP-2 regulates post-endocytic trafficking through the non-clathrin Arf6-dependent endocytic pathway. J. Cell Sci. 121: 4008-4017 [Abstract] [Full Text]
Janvier, K., Bonifacino, J. S. (2005). Role of the Endocytic Machinery in the Sorting of Lysosome-associated Membrane Proteins. Mol. Biol. Cell 16: 4231-4242 [Abstract] [Full Text]
Van Minnebruggen, G., Favoreel, H. W., Nauwynck, H. J. (2004). Internalization of Pseudorabies Virus Glycoprotein B Is Mediated by an Interaction between the YQRL Motif in Its Cytoplasmic Domain and the Clathrin-Associated AP-2 Adaptor Complex. J. Virol. 78: 8852-8859 [Abstract] [Full Text]
Kusner, L., Carlin, C. (2003). Potential role for a novel AP180-related protein during endocytosis in MDCK cells. Am. J. Physiol. Cell Physiol. 285: C995-C1008 [Abstract] [Full Text]
Santini, F., Gaidarov, I., Keen, J. H. (2002). G protein-coupled receptor/arrestin3 modulation of the endocytic machinery. JCB 156: 665-676 [Abstract] [Full Text]
Costaguta, G., Stefan, C. J., Bensen, E. S., Emr, S. D., Payne, G. S. (2001). Yeast Gga Coat Proteins Function with Clathrin in Golgi to Endosome Transport. Mol. Biol. Cell 12: 1885-1896 [Abstract] [Full Text]
Sohn, H. W., Shin, Y. K., Lee, I.-S., Bae, Y. M., Suh, Y. H., Kim, M. K., Kim, T. J., Jung, K. C., Park, W. S., Park, C.-S., Chung, D. H., Ahn, K., Kim, I. S., Ko, Y. H., Bang, Y. J., Kim, C. W., Park, S. H. (2001). CD99 Regulates the Transport of MHC Class I Molecules from the Golgi Complex to the Cell Surface. J. Immunol. 166: 787-794 [Abstract] [Full Text]
Morgan, J. R., Prasad, K., Hao, W., Augustine, G. J., Lafer, E. M. (2000). A Conserved Clathrin Assembly Motif Essential for Synaptic Vesicle Endocytosis. J. Neurosci. 20: 8667-8676 [Abstract] [Full Text]
Santini, F, Penn, R., Gagnon, A., Benovic, J., Keen, J. (2000). Selective recruitment of arrestin-3 to clathrin coated pits upon stimulation of G protein-coupled receptors. J. Cell Sci. 113: 2463-2470 [Abstract]
Engqvist-Goldstein, A. E.Y., Kessels, M. M., Chopra, V. S., Hayden, M. R., Drubin, D. G. (1999). An Actin-Binding Protein of the Sla2/Huntingtin Interacting Protein 1 Family Is a Novel Component of Clathrin-Coated Pits and Vesicles. JCB 147: 1503-1518 [Abstract] [Full Text]
Yeung, B. G., Phan, H. L., Payne, G. S. (1999). Adaptor Complex-independent Clathrin Function in Yeast. Mol. Biol. Cell 10: 3643-3659 [Abstract] [Full Text]
Page, L. J., Sowerby, P. J., Lui, W. W.Y., Robinson, M. S. (1999). {gamma}-Synergin: An Eh Domain-Containing Protein That Interacts with {gamma}-Adaptin. JCB 146: 993-1004 [Abstract] [Full Text]
Hao, W., Luo, Z., Zheng, L., Prasad, K., Lafer, E. M. (1999). AP180 and AP-2 Interact Directly in a Complex That Cooperatively Assembles Clathrin. J. Biol. Chem. 274: 22785-22794 [Abstract] [Full Text]
Hirst, J., Bright, N. A., Rous, B., Robinson, M. S. (1999). Characterization of a Fourth Adaptor-related Protein Complex. Mol. Biol. Cell 10: 2787-2802 [Abstract] [Full Text]
Hussain, N. K., Yamabhai, M., Ramjaun, A. R., Guy, A. M., Baranes, D., O'Bryan, J. P., Der, C. J., Kay, B. K., McPherson, P. S. (1999). Splice Variants of Intersectin Are Components of the Endocytic Machinery in Neurons and Nonneuronal Cells. J. Biol. Chem. 274: 15671-15677 [Abstract] [Full Text]
Journet, A, Chapel, A, Jehan, S, Adessi, C, Freeze, H, Klein, G, Garin, J (1999). Characterization of Dictyostelium discoideum cathepsin D. J. Cell Sci. 112: 3833-3843 [Abstract]
Choice, C. V., Howard, M. J., Poy, M. N., Hankin, M. H., Najjar, S. M. (1998). Insulin Stimulates pp120 Endocytosis in Cells Co-expressing Insulin Receptors. J. Biol. Chem. 273: 22194-22200 [Abstract] [Full Text]
Hannan, L. A., Newmyer, S. L., Schmid, S. L. (1998). ATP- and Cytosol-dependent Release of Adaptor Proteins from Clathrin-coated Vesicles: A Dual Role for Hsc70. Mol. Biol. Cell 9: 2217-2229 [Abstract] [Full Text]
Zhu, Y., Traub, L. M., Kornfeld, S. (1998). ADP-Ribosylation Factor 1�Transiently Activates High-Affinity Adaptor Protein Complex AP-1 Binding Sites On Golgi Membranes. Mol. Biol. Cell 9: 1323-1337 [Abstract] [Full Text]
Benmerah, A., Lamaze, C., Begue, B., Schmid, S. L., Dautry-Varsat, A., Cerf-Bensussan, N. (1998). AP-2/Eps15 Interaction Is Required for Receptor-mediated Endocytosis. JCB 140: 1055-1062 [Abstract] [Full Text]
Tong, X., Boll, W., Kirchhausen, T., Howley, P. M. (1998). Interaction of the Bovine Papillomavirus E6 Protein with the Clathrin Adaptor Complex AP-1. J. Virol. 72: 476-482 [Abstract] [Full Text]
Brown, C., Petersen, N. (1998). An image correlation analysis of the distribution of clathrin associated adaptor protein (AP-2) at the plasma membrane. J. Cell Sci. 111: 271-281 [Abstract]
Kappeler, F., Klopfenstein, D. R.�Ch., Foguet, M., Paccaud, J.-P., Hauri, H.-P. (1997). The Recycling of ERGIC-53 in the Early Secretory Pathway. ERGIC-53 CARRIES A CYTOSOLIC ENDOPLASMIC RETICULUM-EXIT DETERMINANT INTERACTING WITH COPII. J. Biol. Chem. 272: 31801-31808 [Abstract] [Full Text]
Fire, E., Brown, C. M., Roth, M. G., Henis, Y. I., Petersen, N. O. (1997). Partitioning of Proteins into Plasma Membrane Microdomains. CLUSTERING OF MUTANT INFLUENZA VIRUS HEMAGGLUTININS INTO COATED PITS DEPENDS ON THE STRENGTH OF THE INTERNALIZATION SIGNAL. J. Biol. Chem. 272: 29538-29545 [Abstract] [Full Text]
Aguilar, R. C., Ohno, H., Roche, K. W., Bonifacino, J. S. (1997). Functional Domain Mapping of the Clathrin-associated Adaptor Medium Chains �1 and �2. J. Biol. Chem. 272: 27160-27166 [Abstract] [Full Text]
Honing, S., Sosa, M., Hille-Rehfeld, A., von Figura, K. (1997). The 46-kDa Mannose 6-Phosphate Receptor Contains Multiple Binding Sites for Clathrin Adaptors. J. Biol. Chem. 272: 19884-19890 [Abstract] [Full Text]
Ramjaun, A. R., Micheva, K. D., Bouchelet, I., McPherson, P. S. (1997). Identification and Characterization of a Nerve Terminal-enriched Amphiphysin Isoform. J. Biol. Chem. 272: 16700-16706 [Abstract] [Full Text]
Simpson, F., Peden, A. A., Christopoulou, L., Robinson, M. S. (1997). Characterization of the Adaptor-related Protein Complex, AP-3. JCB 137: 835-845 [Abstract] [Full Text]
Vincent, V., Goffin, V., Rozakis-Adcock, M., Mornon, J.-P., Kelly, P. A. (1997). Identification of Cytoplasmic Motifs Required for Short Prolactin Receptor Internalization. J. Biol. Chem. 272: 7062-7068 [Abstract] [Full Text]
Hao, W., Tan, Z., Prasad, K., Reddy, K. K., Chen, J., Prestwich, G. D., Falck, J. R., Shears, S. B., Lafer, E. M. (1997). Regulation of AP-3 Function by Inositides. IDENTIFICATION OF PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE AS A POTENT LIGAND. J. Biol. Chem. 272: 6393-6398 [Abstract] [Full Text]
Delft, S. v., Schumacher, C., Hage, W., Verkleij, A. J., Henegouwen, P. M.P. v. B. e. (1997). Association and Colocalization of Eps15 with Adaptor Protein-2 and Clathrin. JCB 136: 811-821 [Abstract] [Full Text]
Benmerah, A., B�gue, B., Dautry-Varsat, A., Cerf-Bensussan, N. (1996). The Ear of alpha-Adaptin Interacts with the COOH-terminal Domain of the Eps15 Protein. J. Biol. Chem. 271: 12111-12116 [Abstract] [Full Text]
Okabayashi, Y., Sugimoto, Y., Totty, N. F., Hsuan, J., Kido, Y., Sakaguchi, K., Gout, I., Waterfield, M. D., Kasuga, M. (1996). Interaction of Shc with Adaptor Protein Adaptins. J. Biol. Chem. 271: 5265-5269 [Abstract] [Full Text]
Le Borgne, R., Griffiths, G., Hoflack, B. (1996). Mannose 6-Phosphate Receptors and ADP-ribosylation Factors Cooperate for High Affinity Interaction of the AP-1 Golgi Assembly Proteins with Membranes. J. Biol. Chem. 271: 2162-2170 [Abstract] [Full Text]
Gilboa, L., Ben-Levy, R., Yarden, Y. (1995). Roles for a Cytoplasmic Tyrosine and Tyrosine Kinase Activity in the Interactions of Neu Receptors with Coated Pits. J. Biol. Chem. 270: 7061-7067 [Abstract] [Full Text]
Nesterov, A., Kurten, R. C., Gill, G. N. (1995). Association of Epidermal Growth Factor Receptors with Coated Pit Adaptins via a Tyrosine Phosphorylation-regulated Mechanism. J. Biol. Chem. 270: 6320-6327 [Abstract] [Full Text]
Traub, L. M., Kornfeld, S., Ungewickell, E. (1995). Different Domains of the AP-1 Adaptor Complex Are Required for Golgi Membrane Binding and Clathrin Recruitment. J. Biol. Chem. 270: 4933-4942 [Abstract] [Full Text]
Sorkin, A., McKinsey, T., Shih, W., Kirchhausen, T., Carpenter, G. (1995). Stoichiometric Interaction of the Epidermal Growth Factor Receptor with the Clathrin-associated Protein Complex AP-2. J. Biol. Chem. 270: 619-625 [Abstract] [Full Text]
Ball, C., Hunt, S., Robinson, M. (1995). Expression and localization of alpha-adaptin isoforms. J. Cell Sci. 108: 2865-2875 [Abstract]
Norris, F. A., Ungewickell, E., Majerus, P. W. (1995). Inositol Hexakisphosphate Binds to Clathrin Assembly Protein 3 (AP-3/AP180) and Inhibits Clathrin Cage Assembly in Vitro. J. Biol. Chem. 270: 214-217 [Abstract] [Full Text]
Buxbaum, E, Woodman, P. (1995). Selective action of uncoating ATPase towards clathrin-coated vesicles from brain. J. Cell Sci. 108: 1295-1306 [Abstract]
Camidge, D., Pearse, B. (1994). Cloning of Drosophila beta-adaptin and its localization on expression in mammalian cells. J. Cell Sci. 107: 709-718 [Abstract]
Sorkin, A, Carpenter, G (1993). Interaction of activated EGF receptors with coated pit adaptins. Science 261: 612-615 [Abstract]
Draper, R., Goda, Y, Brodsky, F., Pfeffer, S. (1990). Antibodies to clathrin inhibit endocytosis but not recycling to the trans Golgi network in vitro. Science 248: 1539-1541 [Abstract]
Brodsky, F.M., Koppelman, B., Blum, J.S., Marks, M.S., Cresswell, P., Guagliardi, L. (1989). Intracellular Colocalization of Molecules Involved in Antigen Processing and Presentation by B Cells. Cold Spring Harb Symp Quant Biol 54: 319-331 [Abstract]
Brodsky, F. (1988). Living with clathrin: its role in intracellular membrane traffic. Science 242: 1396-1402 [Abstract]
Rotem-Yehudar, R., Galperin, E., Horowitz, M. (2001). Association of Insulin-like Growth Factor 1 Receptor with EHD1 and SNAP29. J. Biol. Chem. 276: 33054-33060 [Abstract] [Full Text]
Keren, T., Roth, M. G., Henis, Y. I. (2001). Internalization-competent Influenza Hemagglutinin Mutants Form Complexes with Clathrin-deficient Multivalent AP-2 Oligomers in Live Cells. J. Biol. Chem. 276: 28356-28363 [Abstract] [Full Text]
Austin, C., Hinners, I., Tooze, S. A. (2000). Direct and GTP-dependent Interaction of ADP-ribosylation Factor 1 with Clathrin Adaptor Protein AP-1 on Immature Secretory Granules. J. Biol. Chem. 275: 21862-21869 [Abstract] [Full Text]
Peden, A. A., Rudge, R. E., Lui, W. W.Y., Robinson, M. S. (2002). Assembly and function of AP-3 complexes in cells expressing mutant subunits. JCB 156: 327-336 [Abstract] [Full Text]
Santini, F., Gaidarov, I., Keen, J. H. (2002). G protein-coupled receptor/arrestin3 modulation of the endocytic machinery. JCB 156: 665-676 [Abstract] [Full Text]