Osteoblasts synthesize and respond to transforming growth factor-type beta (TGF-beta) in vitro

doi:10.1083/jcb.105.1.457

This Article

	Full Text (PDF, 1070K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Robey, P. G.
	Articles by Roberts, A. B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Robey, P. G.
	Articles by Roberts, A. B.


Social Bookmarking

	What's this?

ARTICLES

Osteoblasts synthesize and respond to transforming growth factor-type beta (TGF-beta) in vitro

PG Robey, MF Young, KC Flanders, NS Roche, P Kondaiah, AH Reddi, JD Termine, MB Sporn and AB Roberts

Transforming growth factor-type beta (TGF-beta) has been identified as a constituent of bone matrix (Seyedin, S. M., A. Y. Thompson, H. Bentz, D. M. Rosen, J. M. McPherson, A. Conti, N. R. Siegel, G. R. Gallupi, and K. A. Piez, 1986, J. Biol. Chem. 261:5693-5695). We used both developing bone and bone-forming cells in vitro to demonstrate the cellular origin of this peptide. TGF-beta mRNA was detected by Northern analysis in both developing bone tissue and fetal bovine bone-forming cells using human cDNA probes. TGF-beta was shown to be synthesized and secreted by metabolically labeled bone cell cultures by immunoprecipitation from the medium. Further, TGF-beta activity was demonstrated in conditioned media from these cultures by competitive radioreceptor and growth promotion assays. Fetal bovine bone cells (FBBC) were found to have relatively few TGF-beta receptors (5,800/cell) with an extremely low Kd of 2.2 pM (high binding affinity). In contrast to its inhibitory effects on the growth of many cell types including osteosarcoma cell lines, TGF-beta stimulated the growth of subconfluent cultures of FBBC; it had little effect on the production of collagen by these cells. We conclude that bone-forming cells are a source for the TGF-beta that is found in bone, and that these cells may be modulated by this factor in an autocrine fashion.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Quinlan, J. F., Watson, R. W. G., Kelly, G., Kelly, P. M., O'Byrne, J. M., Fitzpatrick, J. M. (2006). Transforming growth factor-beta (TGF-{beta}) in acute injuries of the spinal cord. J Bone Joint Surg Br 88-B: 406-410 [Abstract] [Full Text]
Janssens, K., ten Dijke, P., Janssens, S., Van Hul, W. (2005). Transforming Growth Factor-{beta}1 to the Bone. Endocr. Rev. 26: 743-774 [Abstract] [Full Text]
Yamamoto, T., Akisue, T., Marui, T., Fujita, I., Matsumoto, K., Hitora, T., Kawamoto, T., Nagira, K., Nakatani, T., Kurosaka, M. (2004). Expression of Transforming Growth Factor {beta} Isoforms and Their Receptors in Malignant Fibrous Histiocytoma of Soft Tissues. Clin. Cancer Res. 10: 5804-5807 [Abstract] [Full Text]
Schrum, L. W., Marriott, I., Butler, B. R., Thomas, E. K., Hudson, M. C., Bost, K. L. (2003). Functional CD40 Expression Induced following Bacterial Infection of Mouse and Human Osteoblasts. Infect. Immun. 71: 1209-1216 [Abstract] [Full Text]
Lieberman, J. R., Daluiski, A., Einhorn, T. A. (2002). The Role of Growth Factors in the Repair of Bone : Biology and Clinical Applications. JBJS 84: 1032-1044 [Full Text]
Fuller, K, Lean, J., Bayley, K., Wani, M., Chambers, T. (2000). A role for TGFbeta(1) in osteoclast differentiation and survival. J. Cell Sci. 113: 2445-2453 [Abstract]
Suzawa, M., Takeuchi, Y., Fukumoto, S., Kato, S., Ueno, N., Miyazono, K., Matsumoto, T., Fujita, T. (1999). Extracellular Matrix-Associated Bone Morphogenetic Proteins Are Essential for Differentiation of Murine Osteoblastic Cells in Vitro. Endocrinology 140: 2125-2133 [Abstract] [Full Text]
Urano, T., Yashiroda, H., Muraoka, M., Tanaka, K., Hosoi, T., Inoue, S., Ouchi, Y., Tanaka, K., Toyoshima, H. (1999). p57Kip2 Is Degraded through the Proteasome in Osteoblasts Stimulated to Proliferation by Transforming Growth Factor beta 1. J. Biol. Chem. 274: 12197-12200 [Abstract] [Full Text]
Filvaroff, E, Erlebacher, A, Ye, J, Gitelman, S., Lotz, J, Heillman, M, Derynck, R (1999). Inhibition of TGF-beta receptor signaling in osteoblasts leads to decreased bone remodeling and increased trabecular bone mass. Development 126: 4267-4279 [Abstract]
Erlebacher, A., Filvaroff, E. H., Ye, J.-Q., Derynck, R. (1998). Osteoblastic Responses to TGF-beta during Bone Remodeling. Mol. Biol. Cell 9: 1903-1918 [Abstract] [Full Text]
Agarwal, S., Chandra, C. S., Piesco, N. P., Langkamp, H. H., Bowen, L., Baran, C. (1998). Regulation of Periodontal Ligament Cell Functions by Interleukin-1beta. Infect. Immun. 66: 932-937 [Abstract] [Full Text]
Guise, T. A., Mundy, G. R. (1998). Cancer and Bone. Endocr. Rev. 19: 18-54 [Abstract] [Full Text]
Pilbeam, C., Rao, Y., Voznesensky, O., Kawaguchi, H., Alander, C., Raisz, L., Herschman, H. (1997). Transforming Growth Factor-{beta}1 Regulation of Prostaglandin G/H Synthase-2 Expression in Osteoblastic MC3T3-E1 Cells. Endocrinology 138: 4672-4682 [Abstract] [Full Text]
Giannobile, W.V., Whitson, S.W., Lynch, S.E. (1997). Non-coordinate Control of Bone Formation Displayed by Growth Factor Combinations with IGF-I. JDR 76: 1569-1578 [Abstract]
Murakami, S., Nifuji, A., Noda, M. (1997). Expression of Indian Hedgehog in Osteoblasts and Its Posttranscriptional Regulation by Transforming Growth Factor-{beta}. Endocrinology 138: 1972-1978 [Abstract] [Full Text]
Quintero, J.C., Piesco, N.P., Langkamp, H.H., Bowen, L., Agarwal, S. (1995). LPS Responsiveness in Periodontal Ligament Cells is Regulated by Tumor Necrosis Factor-{alpha}. JDR 74: 1802-1811 [Abstract]
Critchlow, M., Bland, Y., Ashhurst, D. (1994). The effects of age on the response of rabbit periosteal osteoprogenitor cells to exogenous transforming growth factor-beta 2. J. Cell Sci. 107: 499-516 [Abstract]
Bertolami, C. N., Messadi, D. V. (1994). The Role of Proteoglycans in Hard and Soft Tissue Repair. CROBM 5: 311-337 [Abstract] [Full Text]
Stanford, C. M., Keller, J. C. (1991). The Concept of Osseointegration and Bone Matrix Expression. CROBM 2: 83-101 [Abstract] [Full Text]
Graves, D. T., Cochran, D. L. (1990). Mesenchymal Cell Growth Factors. CROBM 1: 17-36 [Full Text]
Nishimori, S., Tanaka, Y., Chiba, T., Fujii, M., Imamura, T., Miyazono, K., Ogasawara, T., Kawaguchi, H., Igarashi, T., Fujita, T., Tanaka, K., Toyoshima, H. (2001). Smad-mediated Transcription Is Required for Transforming Growth Factor-beta 1-induced p57Kip2 Proteolysis in Osteoblastic Cells. J. Biol. Chem. 276: 10700-10705 [Abstract] [Full Text]