In vivo expression of mRNA for the Ca++-binding protein SPARC (osteonectin) revealed by in situ hybridization

doi:10.1083/jcb.105.1.473

This Article

	Full Text (PDF, 5067K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Holland, P. W.
	Articles by Hogan, B. L.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Holland, P. W.
	Articles by Hogan, B. L.


Social Bookmarking

	What's this?

ARTICLES

In vivo expression of mRNA for the Ca++-binding protein SPARC (osteonectin) revealed by in situ hybridization

PW Holland, SJ Harper, JH McVey and BL Hogan

In situ hybridization is used to survey the tissue-specific and developmental expression of the cloned mouse gene Sparc, coding for a protein homologous to the bovine Ca++-binding protein, osteonectin. High levels of SPARC RNA are found in osteoblasts and odontoblasts. In addition, high grain counts are associated with a variety of other cell types in the embryo and newborn mouse, including parietal endoderm, deciduum, whisker follicles (connective tissue sheath), peripheral nerve trunk, skin (dermis), and stomach (submucosa). Spatially restricted but high levels of SPARC mRNA are also seen in the adult adrenal glands, testis, and ovary. This pattern of differential gene expression demands a reassessment of the function originally proposed for osteonectin, and predicts a much wider role for the protein in a variety of biological processes.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Jorgensen, L. H., Petersson, S. J., Sellathurai, J., Andersen, D. C., Thayssen, S., Sant, D. J., Jensen, C. H., Schroder, H. D. (2009). Secreted Protein Acidic and Rich in Cysteine (SPARC) in Human Skeletal Muscle. J. Histochem. Cytochem. 57: 29-39 [Abstract] [Full Text]
Martinek, N., Shahab, J., Sodek, J., Ringuette, M. (2007). Is SPARC an Evolutionarily Conserved Collagen Chaperone?. JDR 86: 296-305 [Abstract] [Full Text]
Kaji, K., Nichols, J., Hendrich, B. (2007). Mbd3, a component of the NuRD co-repressor complex, is required for development of pluripotent cells. Development 134: 1123-1132 [Abstract] [Full Text]
Ishimoto, H., Ginzinger, D. G., Matsumoto, T., Hattori, Y., Furuya, M., Minegishi, K., Tanaka, M., Yoshimura, Y., Jaffe, R. B. (2006). Differential Zonal Expression and Adrenocorticotropin Regulation of Secreted Protein Acidic and Rich in Cysteine (SPARC), a Matricellular Protein, in the Midgestation Human Fetal Adrenal Gland: Implications for Adrenal Development. J. Clin. Endocrinol. Metab. 91: 3208-3214 [Abstract] [Full Text]
Schellings, M. W.M., Pinto, Y. M., Heymans, S. (2004). Matricellular proteins in the heart: possible role during stress and remodeling. Cardiovasc Res 64: 24-31 [Abstract] [Full Text]
Murakami, A., Shen, H., Ishida, S., Dickson, C. (2004). SOX7 and GATA-4 Are Competitive Activators of Fgf-3 Transcription. J. Biol. Chem. 279: 28564-28573 [Abstract] [Full Text]
Sweetwyne, M. T., Brekken, R. A., Workman, G., Bradshaw, A. D., Carbon, J., Siadak, A. W., Murri, C., Sage, E. H. (2004). Functional Analysis of the Matricellular Protein SPARC with Novel Monoclonal Antibodies. J. Histochem. Cytochem. 52: 723-733 [Abstract] [Full Text]
Yan, Q., Blake, D., Clark, J. I., Sage, E. H. (2003). Expression of the Matricellular Protein SPARC in Murine Lens: SPARC Is Necessary for the Structural Integrity of the Capsular Basement Membrane. J. Histochem. Cytochem. 51: 503-511 [Abstract] [Full Text]
Bradshaw, A. D., Reed, M. J., Sage, E. H. (2002). SPARC-null Mice Exhibit Accelerated Cutaneous Wound Closure. J. Histochem. Cytochem. 50: 1-10 [Abstract] [Full Text]
Yiu, G. K., Chan, W. Y., Ng, S.-W., Chan, P. S., Cheung, K. K., Berkowitz, R. S., Mok, S. C. (2001). SPARC (Secreted Protein Acidic and Rich in Cysteine) Induces Apoptosis in Ovarian Cancer Cells. Am. J. Pathol. 159: 609-622 [Abstract] [Full Text]
McParland, P.C., Bell, S.C., Pringle, J.H., Taylor, D.J. (2001). Regional and cellular localization of osteonectin/SPARC expression in connective tissue and cytotrophoblastic layers of human fetal membranes at term. Mol Hum Reprod 7: 463-474 [Abstract] [Full Text]
Gadeau, A.-P., Chaulet, H., Daret, D., Kockx, M., Daniel-Lamazière, J.-M., Desgranges, C. (2001). Time Course of Osteopontin, Osteocalcin, and Osteonectin Accumulation and Calcification After Acute Vessel Wall Injury. J. Histochem. Cytochem. 49: 79-86 [Abstract] [Full Text]
Lake, J, Rathjen, J, Remiszewski, J, Rathjen, P. (2000). Reversible programming of pluripotent cell differentiation. J. Cell Sci. 113: 555-566 [Abstract]
Bini, A., Mann, K. G., Kudryk, B. J., Schoen, F. J. (1999). Noncollagenous Bone Matrix Proteins, Calcification, and Thrombosis in Carotid Artery Atherosclerosis. Arterioscler. Thromb. Vasc. Bio. 19: 1852-1861 [Abstract] [Full Text]
Yan, Q., Sage, E. H., Hendrickson, A. E. (1998). SPARC Is Expressed by Ganglion Cells and Astrocytes in Bovine Retina. J. Histochem. Cytochem. 46: 3-10 [Abstract] [Full Text]
Hidai, C., Zupancic, T., Penta, K., Mikhail, A., Kawana, M., Quertermous, E. E., Aoka, Y., Fukagawa, M., Matsui, Y., Platika, D., Auerbach, R., Hogan, B. L.M., Snodgrass, R., Quertermous, T. (1998). Cloning and characterization of developmental endothelial locus-1: An embryonic endothelial cell protein that binds the alpha vbeta 3 integrin�receptor. Genes Dev. 12: 21-33 [Abstract] [Full Text]
Soderling, J. A., Reed, M. J., Corsa, A., Sage, E. H. (1997). Cloning and Expression of Murine SC1, a Gene Product Homologous to SPARC. J. Histochem. Cytochem. 45: 823-836 [Abstract] [Full Text]
Xie, R.-L., Long, G. L. (1996). Elements within the First 17 Amino Acids of Human Osteonectin Are Responsible for Binding to Type V Collagen. J. Biol. Chem. 271: 8121-8125 [Abstract] [Full Text]
Greco, T L, Takada, S, Newhouse, M M, McMahon, J A, McMahon, A P, Camper, S A (1996). Analysis of the vestigial tail mutation demonstrates that Wnt-3a gene dosage regulates mouse axial development.. Genes Dev. 10: 313-324 [Abstract]
Hao, H., Hirota, S., Tsukamoto, Y., Imakita, M., Ishibashi-Ueda, H., Yutani, C. (1995). Alterations of Bone Matrix Protein mRNA Expression in Rat Aorta In Vitro. Arterioscler. Thromb. Vasc. Bio. 15: 1474-1480 [Abstract] [Full Text]
Szabo, P, Mann, J. (1994). Expression and methylation of imprinted genes during in vitro differentiation of mouse parthenogenetic and androgenetic embryonic stem cell lines. Development 120: 1651-1660 [Abstract]
Linde, A., Goldberg, M. (1993). Dentinogenesis. CROBM 4: 679-728 [Abstract] [Full Text]
Lukinmaa, P.-L., Vaahtokari, A., Vainio, S., Thesleff, I. (1992). Expression of Type I Collagen Pro-{alpha}2 Chain mRNA in Adult Human Permanent Teeth as Revealed by in situ Hybridization. JDR 71: 36-42 [Abstract]
Bothe, K, Aguzzi, A, Lassmann, H, Rethwilm, A, Horak, I (1991). Progressive encephalopathy and myopathy in transgenic mice expressing human foamy virus genes. Science 253: 555-557 [Abstract]
Milam, S. B., Haskin, C., Zardeneta, G., Dali Chen, , Magnuson, V. L., Klebe, R. J., Steffenson, B. (1991). Cell Adhesion Proteins in Oral Biology. CROBM 2: 451-491 [Full Text]
Flenniken, A M, Williams, B R (1990). Developmental expression of the endogenous TIMP gene and a TIMP-lacZ fusion gene in transgenic mice.. Genes Dev. 4: 1094-1106 [Abstract]
Sauk, J. J. (1990). Stress Proteins in Development And Disease. CROBM 1: 235-245 [Full Text]