The Journal of Cell Biology, Vol 105, 569-576, Copyright © 1987 by The Rockefeller University Press

ARTICLES

Immunoelectron microscopic localization of the neural cell adhesion molecules L1 and N-CAM during postnatal development of the mouse cerebellum

E Persohn and M Schachner

The cellular and subcellular localization of the neural cell adhesion molecules L1 and N-CAM was studied by pre- and postembedding immunoelectron microscopic labeling procedures in the developing mouse cerebellar cortex. The salient features of the study are: L1 displays a previously unrecognized restricted expression by particular neuronal cell types (i.e., it is expressed by granule cells but not by stellate and basket cells) and by particular subcellular compartments (i.e., it is expressed on axons but not on dendrites or cell bodies of Purkinje cells). L1 is always expressed on fasciculating axons and on postmitotic, premigratory, and migrating granule cells at sites of neuron-neuron contact, but never at contact sites between neuron and glia, thus strengthening the view that L1 is not involved in granule cell migration as a neuron-glia adhesion molecule. While N-CAM antibodies reacting with the three major components of N-CAM (180, 140, and 120 kD) show a rather uniform labeling of all cell types, antibodies to the 180-kD component (N-CAM180) stain only the postmigratory granule cell bodies supporting the notion that N-CAM180, the N-CAM component with the longest cytoplasmic domain, is not expressed before stable cell contacts are formed. Furthermore, N-CAM180 is only transiently expressed on Purkinje cell dendrites. N-CAM is present in synapses on both pre- and post-synaptic membranes. L1 is expressed only preterminally and not in the subsynaptic membranes. These observations indicate an exquisite degree of fine tuning in adhesion molecule expression during neural development and suggest a rich combinatorial repertoire in the specification of cell surface contacts.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Maretzky, T., Schulte, M., Ludwig, A., Rose-John, S., Blobel, C., Hartmann, D., Altevogt, P., Saftig, P., Reiss, K. (2005). L1 Is Sequentially Processed by Two Differently Activated Metalloproteases and Presenilin/{gamma}-Secretase and Regulates Neural Cell Adhesion, Cell Migration, and Neurite Outgrowth. Mol. Cell. Biol. 25: 9040-9053 [Abstract] [Full Text]  
• Zhang, Y., Bo, X., Schoepfer, R., Holtmaat, A. J. D. G., Verhaagen, J., Emson, P. C., Lieberman, A. R., Anderson, P. N. (2005). Growth-associated protein GAP-43 and L1 act synergistically to promote regenerative growth of Purkinje cell axons in vivo. Proc. Natl. Acad. Sci. USA 102: 14883-14888 [Abstract] [Full Text]  
• Thelen, K., Kedar, V., Panicker, A. K., Schmid, R.-S., Midkiff, B. R., Maness, P. F. (2002). The Neural Cell Adhesion Molecule L1 Potentiates Integrin-Dependent Cell Migration to Extracellular Matrix Proteins. J. Neurosci. 22: 4918-4931 [Abstract] [Full Text]  
• Hofstra, R M W, Elfferich, P, Osinga, J, Verlind, E, Fransen, E, Lopez Pison, J, de Die-Smulders, C E M, Stolte-Dijkstra, I, Buys, C H C M (2002). Hirschsprung disease and L1CAM: is the disturbed sex ratio caused by L1CAM mutations?. J. Med. Genet. 39: e11-11 [Full Text]  
• Alcantara, S, Ruiz, M, De Castro, F, Soriano, E, Sotelo, C (2000). Netrin 1 acts as an attractive or as a repulsive cue for distinct migrating neurons during the development of the cerebellar system. Development 127: 1359-1372 [Abstract]  
• Takei, K., Chan, T. A., Wang, F.-S., Deng, H., Rutishauser, U., Jay, D. G. (1999). The Neural Cell Adhesion Molecules L1 and NCAM-180 Act in Different Steps of Neurite Outgrowth. J. Neurosci. 19: 9469-9479 [Abstract] [Full Text]  
• Demyanenko, G. P., Tsai, A. Y., Maness, P. F. (1999). Abnormalities in Neuronal Process Extension, Hippocampal Development, and the Ventricular System of L1 Knockout Mice. J. Neurosci. 19: 4907-4920 [Abstract] [Full Text]  
• Buttiglione, M., Revest, J.-M., Pavlou, O., Karagogeos, D., Furley, A., Rougon, G., Faivre-Sarrailh, C. (1998). A Functional Interaction between the Neuronal Adhesion Molecules TAG-1 and F3 Modulates Neurite Outgrowth and Fasciculation of Cerebellar Granule Cells. J. Neurosci. 18: 6853-6870 [Abstract] [Full Text]  
• Kallunki, P., Edelman, G. M., Jones, F. S. (1998). The neural restrictive silencer element can act as both a repressor and enhancer of L1 cell adhesion molecule gene expression during postnatal development. Proc. Natl. Acad. Sci. USA 95: 3233-3238 [Abstract] [Full Text]  
• Yip, P. M., Zhao, X., Montgomery, A. M.P., Siu, C.-H. (1998). The Arg-Gly-Asp Motif in the Cell Adhesion Molecule L1 Promotes Neurite Outgrowth via Interaction with the alpha vbeta 3 Integrin. Mol. Biol. Cell 9: 277-290 [Abstract] [Full Text]  
• Beggs, H. E., Baragona, S. C., Hemperly, J. J., Maness, P. F. (1997). NCAM140 Interacts with the Focal Adhesion Kinase p125fak and the SRC-related Tyrosine Kinase p59fyn. J. Biol. Chem. 272: 8310-8319 [Abstract] [Full Text]  
• Scholey, A B, Mileusnic, R, Schachner, M, Rose, S P (1995). A role for a chicken homolog of the neural cell adhesion molecule L1 in consolidation of memory for a passive avoidance task in the chick.. Learn. Mem. 2: 17-25 [Abstract]  
• Kuhar, S. G., Feng, L., Vidan, S., Ross, M. E., Hatten, M. E., Heintz, N. (1993). Changing patterns of gene expression define four stages of cerebellar granule neuron differentiation. Development 117: 97-104 [Abstract]  
• Tacke, R, Goridis, C (1991). Alternative splicing in the neural cell adhesion molecule pre-mRNA: regulation of exon 18 skipping depends on the 5'-splice site.. Genes Dev. 5: 1416-1429 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents