The Journal of Cell Biology, Vol 105, 1183-1190, Copyright © 1987 by The Rockefeller University Press

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Amino acid sequence of the human fibronectin receptor

WS Argraves, S Suzuki, H Arai, K Thompson, MD Pierschbacher and E Ruoslahti
La Jolla Cancer Research Foundation, California 92037.

The amino acid sequence deduced from cDNA of the human placental fibronectin receptor is reported. The receptor is composed of two subunits: an alpha subunit of 1,008 amino acids which is processed into two polypeptides disulfide bonded to one another, and a beta subunit of 778 amino acids. Each subunit has near its COOH terminus a hydrophobic segment. This and other sequence features suggest a structure for the receptor in which the hydrophobic segments serve as transmembrane domains anchoring each subunit to the membrane and dividing each into a large ectodomain and a short cytoplasmic domain. The alpha subunit ectodomain has five sequence elements homologous to consensus Ca2+- binding sites of several calcium-binding proteins, and the beta subunit contains a fourfold repeat strikingly rich in cysteine. The alpha subunit sequence is 46% homologous to the alpha subunit of the vitronectin receptor. The beta subunit is 44% homologous to the human platelet adhesion receptor subunit IIIa and 47% homologous to a leukocyte adhesion receptor beta subunit. The high degree of homology (85%) of the beta subunit with one of the polypeptides of a chicken adhesion receptor complex referred to as integrin complex strongly suggests that the latter polypeptide is the chicken homologue of the fibronectin receptor beta subunit. These receptor subunit homologies define a superfamily of adhesion receptors. The availability of the entire protein sequence for the fibronectin receptor will facilitate studies on the functions of these receptors.
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• Segat, D., Comai, R., Di Marco, E., Strangio, A., Cancedda, R., Franzi, A. T., Tacchetti, C. (2002). Integrins alpha 6Abeta 1 and alpha 6Bbeta 1 Promote Different Stages of Chondrogenic Cell Differentiation. J. Biol. Chem. 277: 31612-31622 [Abstract] [Full Text]  
• Nishiya, T., Kainoh, M., Murata, M., Handa, M., Ikeda, Y. (2002). Reconstitution of adhesive properties of human platelets in liposomes carrying both recombinant glycoproteins Ia/IIa and Ibalpha under flow conditions: specific synergy of receptor-ligand interactions. Blood 100: 136-142 [Abstract] [Full Text]  
• Jaffe, M. J., Leopold, A. C., Staples, R. C. (2002). Thigmo responses in plants and fungi. Am. J. Bot. 89: 375-382 [Abstract] [Full Text]  
• KRISHNAMURTI, U., ZHOU, B., FAN, W.-W., TSILIBARY, E., WAYNER, E., KIM, Y., KASHTAN, C. E., MICHAEL, A. (2001). Puromycin Aminonucleoside Suppresses Integrin Expression in Cultured Glomerular Epithelial Cells. J. Am. Soc. Nephrol. 12: 758-766 [Abstract] [Full Text]  
• Harris, E. S., Shigeoka, A. O., Li, W., Adams, R. H., Prescott, S. M., McIntyre, T. M., Zimmerman, G. A., Lorant, D. E. (2001). A novel syndrome of variant leukocyte adhesion deficiency involving defects in adhesion mediated by {beta}1 and {beta}2 integrins. Blood 97: 767-776 [Abstract] [Full Text]  
• Valencik, M. L., McDonald, J. A. (2001). Cardiac expression of a gain-of-function {alpha}5-integrin results in perinatal lethality. Am. J. Physiol. Heart Circ. Physiol. 280: H361-H367 [Abstract] [Full Text]  
• Jin, M., He, S., Wörpel, V., Ryan, S. J., Hinton, D. R. (2000). Promotion of Adhesion and Migration of RPE Cells to Provisional Extracellular Matrices by TNF-{alpha}. IOVS 41: 4324-4332 [Abstract] [Full Text]  
• Bowen, J. A., Hunt, J. S. (2000). The Role of Integrins in Reproduction. Exp. Biol. Med. 223: 331-343 [Abstract] [Full Text]  
• Baneres, J.-L., Roquet, F., Martin, A., Parello, J. (2000). A Minimized Human Integrin alpha 5beta 1 That Retains Ligand Recognition. J. Biol. Chem. 275: 5888-5903 [Abstract] [Full Text]  
• Levy, L., Broad, S., Diekmann, D., Evans, R. D., Watt, F. M. (2000). beta 1 Integrins Regulate Keratinocyte Adhesion and Differentiation by Distinct Mechanisms. Mol. Biol. Cell 11: 453-466 [Abstract] [Full Text]  
• Adachi, M., Taki, T., Higashiyama, M., Kohno, N., Inufusa, H., Miyake, M. (2000). Significance of Integrin {{alpha}}5 Gene Expression as a Prognostic Factor in Node-negative Non-Small Cell Lung Cancer. Clin. Cancer Res. 6: 96-101 [Abstract] [Full Text]  
• Armulik, A., Nilsson, I., von Heijne, G., Johansson, S. (1999). Determination of the Border between the Transmembrane and Cytoplasmic Domains of Human Integrin Subunits. J. Biol. Chem. 274: 37030-37034 [Abstract] [Full Text]  
• Gimond, C., van der Flier, A., van Delft, S., Brakebusch, C., Kuikman, I., Collard, J. G., Fassler, R., Sonnenberg, A. (1999). Induction of Cell Scattering by Expression of {beta}1 Integrins in {beta}1-Deficient Epithelial Cells Requires Activation of Members of the Rho Family of Gtpases and Downregulation of Cadherin and Catenin Function. JCB 147: 1325-1340 [Abstract] [Full Text]  
• Clemetson, J. M., Polgar, J., Magnenat, E., Wells, T. N. C., Clemetson, K. J. (1999). The Platelet Collagen Receptor Glycoprotein VI Is a Member of the Immunoglobulin Superfamily Closely Related to Fcalpha R and the Natural Killer Receptors. J. Biol. Chem. 274: 29019-29024 [Abstract] [Full Text]  
• Patil, S., Jedsadayanmata, A., Wencel-Drake, J. D., Wang, W., Knezevic, I., Lam, S. C.-T. (1999). Identification of a Talin-binding Site in the Integrin beta 3 Subunit Distinct from the NPLY Regulatory Motif of Post-ligand Binding Functions. THE TALIN N-TERMINAL HEAD DOMAIN INTERACTS WITH THE MEMBRANE-PROXIMAL REGION OF THE beta 3 CYTOPLASMIC TAIL. J. Biol. Chem. 274: 28575-28583 [Abstract] [Full Text]  
• Asthagiri, A. R., Nelson, C. M., Horwitz, A. F., Lauffenburger, D. A. (1999). Quantitative Relationship among Integrin-Ligand Binding, Adhesion, and Signaling via Focal Adhesion Kinase and Extracellular Signal-regulated Kinase 2. J. Biol. Chem. 274: 27119-27127 [Abstract] [Full Text]  
• Haque Gazi, M., Ito, M. (1999). Use of A Novel Fibronectin Receptor for Liver Infiltration by a Mouse Lymphoma Cell Line RL-{}1. Cancer Res. 59: 1115-1119 [Abstract] [Full Text]  
• Svineng, G, Johansson, S (1999). Integrin subunits (beta)1C-1 and (beta)1C-2 expressed in GD25T cells are retained and degraded intracellularly rather than localised to the cell surface. J. Cell Sci. 112: 4751-4761 [Abstract]  
• Zinkl, G., Zwiebel, B., Grier, D., Preuss, D (1999). Pollen-stigma adhesion in Arabidopsis: a species-specific interaction mediated by lipophilic molecules in the pollen exine. Development 126: 5431-5440 [Abstract]  
• Collo, G, Pepper, M. (1999). Endothelial cell integrin alpha5beta1 expression is modulated by cytokines and during migration in vitro. J. Cell Sci. 112: 569-578 [Abstract]  
• Ozaki, I, Yamamoto, K, Mizuta, T, Kajihara, S, Fukushima, N, Setoguchi, Y, Morito, F, Sakai, T (1998). Differential expression of laminin receptors in human hepatocellular carcinoma. Gut 43: 837-842 [Abstract] [Full Text]  
• Nakamura, I., Tanaka, H., Rodan, G. A., Duong, L. T. (1998). Echistatin Inhibits the Migration of Murine Prefusion Osteoclasts and the Formation of Multinucleated Osteoclast-Like Cells. Endocrinology 139: 5182-5193 [Abstract] [Full Text]  
• Taverna, D., Disatnik, M.-H., Rayburn, H., Bronson, R. T., Yang, J., Rando, T. A., Hynes, R. O. (1998). Dystrophic Muscle in Mice Chimeric for Expression of {alpha}5 Integrin. JCB 143: 849-859 [Abstract] [Full Text]  
• Baneres, J.-L., Roquet, F., Green, M., LeCalvez, H., Parello, J. (1998). The Cation-binding Domain from the alpha  Subunit of Integrin alpha 5beta 1 Is a Minimal Domain for Fibronectin Recognition. J. Biol. Chem. 273: 24744-24753 [Abstract] [Full Text]  
• Jabbour, A. J., Altman, L. C., Wight, T. N., Luchtel, D. L. (1998). Ozone Alters the Distribution of beta 1 Integrins in Cultured Primate Bronchial Epithelial Cells. Am. J. Respir. Cell Mol. Bio. 19: 357-365 [Abstract] [Full Text]  
• Zuk, A., Bonventre, J. V., Brown, D., Matlin, K. S. (1998). Polarity, integrin, and extracellular matrix dynamics in the postischemic rat kidney. Am. J. Physiol. Cell Physiol. 275: C711-C731 [Abstract] [Full Text]  
• Camper, L., Hellman, U., Lundgren-Akerlund, E. (1998). Isolation, Cloning, and Sequence Analysis of the Integrin Subunit alpha 10, a beta 1-associated Collagen Binding Integrin Expressed on Chondrocytes. J. Biol. Chem. 273: 20383-20389 [Abstract] [Full Text]  
• Chen, Y., Garrison, S., Weis, J. J., Weis, J. H. (1998). Identification of Pactolus, an Integrin beta  Subunit-like Cell-surface Protein Preferentially Expressed by Cells of the Bone Marrow. J. Biol. Chem. 273: 8711-8718 [Abstract] [Full Text]  
• Palecek, S., Huttenlocher, A, Horwitz, A., Lauffenburger, D. (1998). Physical and biochemical regulation of integrin release during rear detachment of migrating cells. J. Cell Sci. 111: 929-940 [Abstract]  
• Belkin, A. M., Retta, S. F., Pletjushkina, O. Y., Balzac, F., Silengo, L., Fassler, R., Koteliansky, V. E., Burridge, K., Tarone, G. (1997). Muscle {beta}1D Integrin Reinforces the Cytoskeleton-Matrix Link: Modulation of Integrin Adhesive Function by Alternative Splicing. JCB 139: 1583-1595 [Abstract] [Full Text]  
• Pulli, T., Koivunen, E., Hyypia, T. (1997). Cell-surface Interactions of Echovirus 22. J. Biol. Chem. 272: 21176-21180 [Abstract] [Full Text]  
• Brower, D. L., Brower, S. M., Hayward, D. C., Ball, E. E. (1997). Molecular evolution of integrins: Genes encoding integrin beta  subunits from a coral and a sponge. Proc. Natl. Acad. Sci. USA 94: 9182-9187 [Abstract] [Full Text]  
• Hultgardh-Nilsson, A., Lovdahl, C., Blomgren, K., Bengt Kallin, , Thyberg, J. (1997). Expression of phenotype- and proliferation-related genes in rat aortic smooth muscle cells in primary culture. Cardiovasc Res 34: 418-430 [Abstract] [Full Text]  
• McKay, B. S., Annis, D. S., Honda, S., Christie, D., Kunicki, T. J. (1996). Molecular Requirements for Assembly and Function of a Minimized Human Integrin alpha IIbbeta 3. J. Biol. Chem. 271: 30544-30547 [Abstract] [Full Text]  
• Goodman, T. G., Bajt, M. L. (1996). Identifying the Putative Metal Ion-dependent Adhesion Site in the beta 2 (CD18) Subunit Required for alpha Lbeta 2 and alpha Mbeta 2 Ligand Interactions. J. Biol. Chem. 271: 23729-23736 [Abstract] [Full Text]  
• Puzon-McLaughlin, W., Takada, Y. (1996). Critical Residues for Ligand Binding in an I Domain-like Structure of the Integrin beta 1 Subunit. J. Biol. Chem. 271: 20438-20443 [Abstract] [Full Text]  
• Puzon-McLaughlin, W., Yednock, T. A., Takada, Y. (1996). Regulation of Conformation and Ligand Binding Function of Integrin alpha 5beta 1 by the beta 1 Cytoplasmic Domain. J. Biol. Chem. 271: 16580-16585 [Abstract] [Full Text]  
• Luque, A., Gómez, M., Puzon, W., Takada, Y., Sánchez-Madrid, F., Cabañas, C. (1996). Activated Conformations of Very Late Activation Integrins Detected by a Group of Antibodies (HUTS) Specific for a Novel Regulatory Region(355-425) of the Common beta1 Chain. J. Biol. Chem. 271: 11067-11075 [Abstract] [Full Text]  
• Zhang, S D, Kassis, J, Olde, B, Mellerick, D M, Odenwald, W F (1996). Pollux, a novel Drosophila adhesion molecule, belongs to a family of proteins expressed in plants, yeast, nematodes, and man.. Genes Dev. 10: 1108-1119 [Abstract]  
• Wilkins, J. A., Li, A., Ni, H., Stupack, D. G., Shen, C. (1996). Control of beta(1) Integrin Function. J. Biol. Chem. 271: 3046-3051 [Abstract] [Full Text]  
• Bazzoni, G., Shih, D.-T., Buck, C. A., Hemler, M. E. (1995). Monoclonal Antibody 9EG7 Defines a Novel beta(1) Integrin Epitope Induced by Soluble Ligand and Manganese, but Inhibited by Calcium. J. Biol. Chem. 270: 25570-25577 [Abstract] [Full Text]  
• Vuori, K., Ruoslahti, E. (1995). Tyrosine Phosphorylation of p130[IMAGE] and Cortactin Accompanies Integrin-mediated Cell Adhesion to Extracellular Matrix. J. Biol. Chem. 270: 22259-22262 [Abstract] [Full Text]  
• O'Toole, T. E., Ylanne, J., Culley, B. M. (1995). Regulation of Integrin Affinity States through an NP XY Motif in the [IMAGE] Subunit Cytoplasmic Domain. J. Biol. Chem. 270: 8553-8558 [Abstract] [Full Text]  
• Albiges-Rizo, C, Frachet, P, Block, M. (1995). Down regulation of talin alters cell adhesion and the processing of the alpha 5 beta 1 integrin. J. Cell Sci. 108: 3317-3329 [Abstract]  
• Bajt, M. L., Goodman, T., McGuire, S. L. (1995). beta(2)(CD18) Mutations Abolish Ligand Recognition by I Domain Integrins LFA-1 (alpha(L)beta(2), CD11a/CD18) and MAC-1 (alpha(M)beta(2), CD11b/CD18). J. Biol. Chem. 270: 94-98 [Abstract] [Full Text]  
• Gladson, C., Wilcox, J., Sanders, L, Gillespie, G., Cheresh, D. (1995). Cerebral microenvironment influences expression of the vitronectin gene in astrocytic tumors. J. Cell Sci. 108: 947-956 [Abstract]  
• Schnapp, L., Breuss, J., Ramos, D., Sheppard, D, Pytela, R (1995). Sequence and tissue distribution of the human integrin alpha 8 subunit: a beta 1-associated alpha subunit expressed in smooth muscle cells. J. Cell Sci. 108: 537-544 [Abstract]  
• Schoenenberger, C., Zuk, A, Zinkl, G., Kendall, D, Matlin, K. (1994). Integrin expression and localization in normal MDCK cells and transformed MDCK cells lacking apical polarity. J. Cell Sci. 107: 527-541 [Abstract]  
• Lallier, T, Bronner-Fraser, M (1993). Inhibition of neural crest cell attachment by integrin antisense oligonucleotides. Science 259: 692-695 [Abstract]  
• Tranqui, L, Usson, Y, Marie, C, Block, M. (1993). Adhesion of CHO cells to fibronectin is mediated by functionally and structurally distinct adhesion plaques. J. Cell Sci. 106: 377-387 [Abstract]  
• Sriramarao, P, Mendler, M, Bourdon, M. (1993). Endothelial cell attachment and spreading on human tenascin is mediated by alpha 2 beta 1 and alpha v beta 3 integrins. J. Cell Sci. 105: 1001-1012 [Abstract]  
• Yee, G., Hynes, R. (1993). A novel, tissue-specific integrin subunit, beta nu, expressed in the midgut of Drosophila melanogaster. Development 118: 845-858 [Abstract]  
• Pasqualini, R, Bodorova, J, Ye, S, Hemler, M. (1993). A study of the structure, function and distribution of beta 5 integrins using novel anti-beta 5 monoclonal antibodies. J. Cell Sci. 105: 101-111 [Abstract]  
• Whittaker, C., DeSimone, D. (1993). Integrin alpha subunit mRNAs are differentially expressed in early Xenopus embryos. Development 117: 1239-1249 [Abstract]  
• Kim, L. T., Ishihara, S., Lee, C. C., Akiyama, S. K., Yamada, K. M., Grinnell, F. (1992). Altered glycosylation and cell surface expression of beta 1 integrin receptors during keratinocyte activation. J. Cell Sci. 103: 743-753 [Abstract]  
• Bergelson, J., Shepley, M., Chan, B., Hemler, M., Finberg, R. (1992). Identification of the integrin VLA-2 as a receptor for echovirus 1. Science 255: 1718-1720 [Abstract]  
• Hemler, M.E., Kassner, P.D., Chan, B.M.C. (1992). Functional Roles for Integrin {alpha} Subunit Cytoplasmic Domains. Cold Spring Harb Symp Quant Biol 57: 213-220 [Abstract]  
• Ginsberg, M.H., O'Toole, T.E., Loftus, J.C., Plow, E.F. (1992). Ligand Binding to Integrins: Dynamic Regulation and Common Mechanisms. Cold Spring Harb Symp Quant Biol 57: 221-231 [Abstract]  
• Hu, M.C.-T., Siegelman, M.H., Holzmann, B., Crowe, D.T., Weissman, I.L. (1992). Lymphocyte Homing Receptors. Cold Spring Harb Symp Quant Biol 57: 291-308 [Abstract]  
• Scallon, B., Fung, W., Tsang, T., Li, S, Kado-Fong, H, Huang, K., Kochan, J. (1991). Primary structure and functional activity of a phosphatidylinositol-glycan-specific phospholipase D. Science 252: 446-448 [Abstract]  
• Milam, S. B., Haskin, C., Zardeneta, G., Dali Chen, , Magnuson, V. L., Klebe, R. J., Steffenson, B. (1991). Cell Adhesion Proteins in Oral Biology. CROBM 2: 451-491 [Full Text]  
• Loftus, J., O'Toole, T., Plow, E., Glass, A, Frelinger, A. 3rd, Ginsberg, M. (1990). A beta 3 integrin mutation abolishes ligand binding and alters divalent cation-dependent conformation. Science 249: 915-918 [Abstract]  
• D'Souza, S., Ginsberg, M., Burke, T., Lam, S., Plow, E. (1988). Localization of an Arg-Gly-Asp recognition site within an integrin adhesion receptor. Science 242: 91-93 [Abstract]  
• Gehlsen, K., Dillner, L, Engvall, E, Ruoslahti, E (1988). The human laminin receptor is a member of the integrin family of cell adhesion receptors. Science 241: 1228-1229 [Abstract]  
• Ruoslahti, E, Pierschbacher, M. (1987). New perspectives in cell adhesion: RGD and integrins. Science 238: 491-497 [Abstract]  
• Nadanaka, S., Sato, C., Kitajima, K., Katagiri, K., Irie, S., Yamagata, T. (2001). Occurrence of Oligosialic Acids on Integrin alpha 5 Subunit and Their Involvement in Cell Adhesion to Fibronectin. J. Biol. Chem. 276: 33657-33664 [Abstract] [Full Text]  
• Kessler, D., Dethlefsen, S., Haase, I., Plomann, M., Hirche, F., Krieg, T., Eckes, B. (2001). Fibroblasts in Mechanically Stressed Collagen Lattices Assume a "Synthetic" Phenotype. J. Biol. Chem. 276: 36575-36585 [Abstract] [Full Text]  

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