Isotypes of alpha-tubulin are differentially regulated during neuronal maturation

doi:10.1083/jcb.105.6.3065

This Article

	Full Text (PDF, 2592K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Miller, F. D.
	Articles by Milner, R. J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Miller, F. D.
	Articles by Milner, R. J.


Social Bookmarking

	What's this?

ARTICLES

Isotypes of alpha-tubulin are differentially regulated during neuronal maturation

FD Miller, CC Naus, M Durand, FE Bloom and RJ Milner
Division of Preclinical Neuroscience and Endocrinology, Research Institute of Scripps Clinic, La Jolla, California 92037.

The mRNAs for two isotypes of alpha-tubulin, termed T alpha 1 and T26, are known to be expressed in the rat nervous system. We have compared the expression of these two alpha-tubulin mRNAs during neural development, using RNA blotting and in situ hybridization techniques with probes directed against unique sequences of each mRNA. T alpha 1 mRNA is highly enriched in the embryonic nervous system but is markedly less abundant in the adult brain; T26 mRNA is expressed in many embryonic tissues with little change in abundance during development. Within the nervous system, T alpha 1 mRNA is enriched in regions with neurons actively undergoing neurite extension, such as the cortical plate, whereas T26 mRNA is relatively homogeneous in distribution, with some enrichment in proliferative zones. Expression of T alpha 1 mRNA is also increased in PC12 cells induced to differentiate and extend neurite processes by nerve growth factor. Taken together, the data indicate that T alpha 1-tubulin mRNA is expressed at high levels during the extension of neuronal processes. The abundant expression of T alpha 1-tubulin mRNA may therefore reflect either a means to increase the available pool of alpha-tubulin or a specific requirement for the T alpha 1 isotype for neurite extension.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Porayette, P., Gallego, M. J., Kaltcheva, M. M., Bowen, R. L., Vadakkadath Meethal, S., Atwood, C. S. (2009). Differential Processing of Amyloid-{beta} Precursor Protein Directs Human Embryonic Stem Cell Proliferation and Differentiation into Neuronal Precursor Cells. J. Biol. Chem. 284: 23806-23817 [Abstract] [Full Text]
Wienecke, A., Bacher, G. (2009). Indibulin, a Novel Microtubule Inhibitor, Discriminates between Mature Neuronal and Nonneuronal Tubulin. Cancer Res. 69: 171-177 [Abstract] [Full Text]
Paquin, A., Barnabe-Heider, F., Kageyama, R., Miller, F. D. (2005). CCAAT/Enhancer-Binding Protein Phosphorylation Biases Cortical Precursors to Generate Neurons Rather Than Astrocytes In Vivo. J. Neurosci. 25: 10747-10758 [Abstract] [Full Text]
Senut, M.-C., Gulati-Leekha, A., Goldman, D. (2004). An Element in the {alpha}1-Tubulin Promoter Is Necessary for Retinal Expression during Optic Nerve Regeneration But Not after Eye Injury in the Adult Zebrafish. J. Neurosci. 24: 7663-7673 [Abstract] [Full Text]
Moccia, R., Chen, D., Lyles, V., Kapuya, E., E, Y., Kalachikov, S., Spahn, C. M. T., Frank, J., Kandel, E. R., Barad, M., Martin, K. C. (2003). An Unbiased cDNA Library Prepared from Isolated Aplysia Sensory Neuron Processes Is Enriched for Cytoskeletal and Translational mRNAs. J. Neurosci. 23: 9409-9417 [Abstract] [Full Text]
THIRIET, N., LADENHEIM, B., McCOY, M. T., CADET, J. L. (2002). Analysis of Ecstasy (MDMA)-induced transcriptional responses in the rat cortex. FASEB J. 16: 1887-1894 [Abstract] [Full Text]
Slack, R. S., El-Bizri, H., Wong, J., Belliveau, D. J., Miller, F. D. (1998). A Critical Temporal Requirement for the Retinoblastoma Protein Family During Neuronal Determination. JCB 140: 1497-1509 [Abstract] [Full Text]
Kobayashi, N. R., Fan, D.-P., Giehl, K. M., Bedard, A. M., Wiegand, S. J., Tetzlaff, W. (1997). BDNF and NT-4/5 Prevent Atrophy of Rat Rubrospinal Neurons after Cervical Axotomy, Stimulate GAP-43 and Talpha 1-Tubulin mRNA Expression, and Promote Axonal Regeneration. J. Neurosci. 17: 9583-9595 [Abstract] [Full Text]
Fournier, A. E., McKerracher, L. (1997). Expression of Specific Tubulin Isotypes Increases during Regeneration of Injured CNS Neurons, But Not after the Application of Brain-Derived Neurotrophic Factor (BDNF). J. Neurosci. 17: 4623-4632 [Abstract] [Full Text]
Belliveau, D. J., Krivko, I., Kohn, J., Lachance, C., Pozniak, C., Rusakov, D., Kaplan, D., Miller, F. D. (1997). NGF and Neurotrophin-3 Both Activate TrkA on Sympathetic Neurons but Differentially Regulate Survival and Neuritogenesis. JCB 136: 375-388 [Abstract] [Full Text]
Audebert, S, Koulakoff, A, Berwald-Netter, Y, Gros, F, Denoulet, P, Edde, B (1994). Developmental regulation of polyglutamylated alpha- and beta-tubulin in mouse brain neurons. J. Cell Sci. 107: 2313-2322 [Abstract]
Jiang, Y. Q., Oblinger, M. M. (1992). Differential regulation of beta III and other tubulin genes during peripheral and central neuron development. J. Cell Sci. 103: 643-651 [Abstract]