Inhibition of in vitro tumor cell invasion by Arg-Gly-Asp-containing synthetic peptides [published erratum appears in J Cell Biol 1989 Jun;108(6):following 2546]

doi:10.1083/jcb.106.3.925

This Article

	Full Text (PDF, 836K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Gehlsen, K. R.
	Articles by Ruoslahti, E.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Gehlsen, K. R.
	Articles by Ruoslahti, E.


Social Bookmarking

	What's this?

ARTICLES

Inhibition of in vitro tumor cell invasion by Arg-Gly-Asp-containing synthetic peptides [published erratum appears in J Cell Biol 1989 Jun;108(6):following 2546]

KR Gehlsen, WS Argraves, MD Pierschbacher and E Ruoslahti
Cancer Research Center, La Jolla Cancer Research Foundation, California 92037.

The interaction of cells with extracellular matrix components such as fibronectin, vitronectin, and type I collagen has been shown to be mediated through a family of cell-surface receptors that specifically recognize an arginine-glycine-aspartic acid (RGD) amino acid sequence within each protein. Synthetic peptides containing the RGD sequence can inhibit these receptor-ligand interactions. Here, we use novel RGD- containing synthetic peptides with different inhibition properties to investigate the role of the various RGD receptors in tumor cell invasion. The RGD-containing peptides used include peptides that inhibit the attachment of cells to fibronectin and vitronectin, a peptide that inhibits attachment to fibronectin but not to vitronectin, a cyclic peptide with the opposite specificity, and a peptide, GRGDTP, that inhibits attachment to type I collagen in addition to inhibiting attachment to fibronectin and vitronectin. The penetration of two human melanoma cell lines and a glioblastoma cell line through the human amniotic basement membrane and its underlying stroma was inhibited by all of the RGD-containing peptides except for the one that inhibits only the vitronectin attachment. Various control peptides lacking RGD showed essentially no inhibition. This inhibitory effect on cell invasion was dose-dependent and nontoxic. A hexapeptide, GRGDTP, that inhibits the attachment of cells to type I collagen in addition to inhibiting fibronectin- and vitronectin-mediated attachment was more inhibitory than those RGD peptides that inhibit only fibronectin and vitronectin attachment. Analysis of the location of these cells that were prevented from invading indicated that they attached to the amniotic basement membrane but did not proceed further into the tissue. These results suggest that interactions between RGD-containing extracellular matrix adhesion proteins and cells are necessary for cell invasion through tissues and that fibronectin and type I collagen are important for this process.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Taylor, B. N., Mehta, R. R., Yamada, T., Lekmine, F., Christov, K., Chakrabarty, A. M., Green, A., Bratescu, L., Shilkaitis, A., Beattie, C. W., Das Gupta, T. K. (2009). Noncationic Peptides Obtained From Azurin Preferentially Enter Cancer Cells. Cancer Res. 69: 537-546 [Abstract] [Full Text]
Torres, A. J., Vasudevan, L., Holowka, D., Baird, B. A. (2008). Nanomaterials in Medicine Special Feature Sackler Colloquium: Focal adhesion proteins connect IgE receptors to the cytoskeleton as revealed by micropatterned ligand arrays. Proc. Natl. Acad. Sci. USA 105: 17238-17244 [Abstract] [Full Text]
Cao, W., Mah, K., Carroll, R.S., Slayden, O.D., Brenner, R.M. (2007). Progesterone withdrawal up-regulates fibronectin and integrins during menstruation and repair in the rhesus macaque endometrium. Hum Reprod 22: 3223-3231 [Abstract] [Full Text]
Ebrahimnejad, A., Streichert, T., Nollau, P., Horst, A. K., Wagener, C., Bamberger, A.-M., Brummer, J. (2004). CEACAM1 Enhances Invasion and Migration of Melanocytic and Melanoma Cells. Am. J. Pathol. 165: 1781-1787 [Abstract] [Full Text]
Lee, T.-H., Avraham, H. K., Jiang, S., Avraham, S. (2003). Vascular Endothelial Growth Factor Modulates the Transendothelial Migration of MDA-MB-231 Breast Cancer Cells through Regulation of Brain Microvascular Endothelial Cell Permeability. J. Biol. Chem. 278: 5277-5284 [Abstract] [Full Text]
Zygmunt, M., Herr, F., Keller-Schoenwetter, S., Kunzi-Rapp, K., Munstedt, K., Rao, C. V., Lang, U., Preissner, K. T. (2002). Characterization of Human Chorionic Gonadotropin as a Novel Angiogenic Factor. J. Clin. Endocrinol. Metab. 87: 5290-5296 [Abstract] [Full Text]
Munevar, S., Wang, Y.-l., Dembo, M. (2001). Distinct Roles of Frontal and Rear Cell-Substrate Adhesions in Fibroblast Migration. Mol. Biol. Cell 12: 3947-3954 [Abstract] [Full Text]
Chen, J. J. W., Peck, K., Hong, T.-M., Yang, S.-C., Sher, Y.-P., Shih, J.-Y., Wu, R., Cheng, J.-L., Roffler, S. R., Wu, C.-W., Yang, P.-C. (2001). Global Analysis of Gene Expression in Invasion by a Lung Cancer Model. Cancer Res. 61: 5223-5230 [Abstract] [Full Text]
Thomas, G., Speight, P.M. (2001). Cell Adhesion Molecules and Oral Cancer. CROBM 12: 479-498 [Abstract] [Full Text]
Koks, C. A.M., Groothuis, P. G., Dunselman, G. A.J., de Goeij, A. F.P.M., Evers, J. L.H. (2000). Adhesion of menstrual endometrium to extracellular matrix: the possible role of integrin {alpha}6{beta}1 and laminin interaction. Mol Hum Reprod 6: 170-177 [Abstract] [Full Text]
Nishizaka, T., Shi, Q., Sheetz, M. P. (2000). Position-dependent linkages of fibronectin- integrin-cytoskeleton. Proc. Natl. Acad. Sci. USA 97: 692-697 [Abstract] [Full Text]
Brakebusch, C, Hirsch, E, Potocnik, A, Fassler, R (1997). Genetic analysis of beta1 integrin function: confirmed, new and revised roles for a crucial family of cell adhesion molecules. J. Cell Sci. 110: 2895-2904 [Abstract]
Chan, B., Matsuura, N, Takada, Y, Zetter, B., Hemler, M. (1991). In vitro and in vivo consequences of VLA-2 expression on rhabdomyosarcoma cells. Science 251: 1600-1602 [Abstract]
Uitto, V.-J., Larjava, H. (1991). Extracellular Matrix Molecules and their Receptors: An Overview with Special Emphasis on Periodontal Tissues. CROBM 2: 323-354 [Abstract] [Full Text]
Terranova, V.P., Jendresen, M., Young, F. (1989). Healing, Regeneration, and Repair: Prospectus for New Dental Treatment. ADR 3: 69-79 [Abstract]
Chassagne, C., Adamy, C., Ratajczak, P., Gingras, B., Teiger, E., Planus, E., Oliviero, P., Rappaport, L., Samuel, J.-L., Meloche, S. (2002). Angiotensin II AT2 receptor inhibits smooth muscle cell migration via fibronectin cell production and binding. Am. J. Physiol. Cell Physiol. 282: C654-C664 [Abstract] [Full Text]