The Journal of Cell Biology, Vol 107, 115-120, Copyright © 1988 by The Rockefeller University Press

ARTICLES

Yeast vacuoles fragment when microtubules are disrupted

BA Guthrie and W Wickner
Molecular Biology Institute, University of California, Los Angeles 90024.

To identify whether microtubules are involved in the maintenance of vacuolar morphology, we treated Saccharomyces cerevisiae with nocodazole and methyl benzimidazole-2-yl-carbamate, drugs which inhibit the polymerization of microtubules. Treated cells arrest with a single large bud in the G2/prophase portion of the cell cycle. Labeling the vacuole with either quinacrine or FITC-dextran revealed vacuole fragmentation that was not found in untreated cells or in cells arrested in G2 by unrelated means. A drug-resistant mutant in beta tubulin does not show vacuolar fragmentation when treated with drug. We propose that microtubules are involved in the regulation of vacuole morphology.
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