Molecular cloning of cDNA for caltractin, a basal body-associated Ca2+- binding protein: homology in its protein sequence with calmodulin and the yeast CDC31 gene product

doi:10.1083/jcb.107.1.133

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Molecular cloning of cDNA for caltractin, a basal body-associated Ca2+- binding protein: homology in its protein sequence with calmodulin and the yeast CDC31 gene product

B Huang, A Mengersen and VD Lee
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.

An extended synthetic oligonucleotide (59-mer) was used to isolate a Chlamydomonas cDNA containing the entire coding region for a novel basal body-associated 20-kD calcium-binding protein (CaBP). DNA and RNA blot analysis indicate that the 20-kD CaBP is encoded by a single copy gene from which is derived an approximately 1.1-kb-long transcript. The deduced amino acid sequence for the protein shows a linear relatedness with calmodulin from Chlamydomonas and other organisms (45-48% identity). The primary protein sequence of the 20-kD CaBP and its predicted secondary structure suggests that the protein is likely to contain four homologous calcium-binding domains that conform to the helix-loop-helix (or EF hand) structure found in calmodulin and related calcium-modulated proteins. The major difference between the protein and calmodulin is an amino-terminal domain of 21 amino acids present on the 20-kD CaBP. In addition to its relatedness to calmodulin, the Chlamydomonas 20-kD CaBP shows a strong sequence identity (50%) with the yeast Saccharomyces cerevisiae CDC31 gene product required for spindle pole body duplication. The association of these sequence- related calcium-binding proteins to microtubule-organizing centers of divergent structure suggests a potential conserved function for the proteins.
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