Signal transduction in Dictyostelium fgd A mutants with a defective interaction between surface cAMP receptors and a GTP-binding regulatory protein [published erratum appears in J Cell Biol 1988 Dec;107(6 Pt 1):following 2463]

doi:10.1083/jcb.107.2.521

This Article

Full Text (PDF, 886K)

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JCB

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Kesbeke, F.

Articles by Van Haastert, P. J.

Search for Related Content

PubMed

PubMed Citation

Articles by Kesbeke, F.

Articles by Van Haastert, P. J.

Pubmed/NCBI databases

Compound via MeSH
Substance via MeSH

Social Bookmarking

What's this?

ARTICLES

Signal transduction in Dictyostelium fgd A mutants with a defective interaction between surface cAMP receptors and a GTP-binding regulatory protein [published erratum appears in J Cell Biol 1988 Dec;107(6 Pt 1):following 2463]

F Kesbeke, BE Snaar-Jagalska and PJ Van Haastert
Department of Biology, University of Leiden, The Netherlands.

Transmembrane signal transduction was investigated in four Dictyostelium discoideum mutants that belong to the fgd A complementation group. The results show the following. (a) Cell surface cAMP receptors are present in fgd A mutants, but cAMP does not induce any of the intracellular responses, including the activation of adenylate or guanylate cyclase and chemotaxis. (b) cAMP induces down- regulation and the covalent modification (presumably phosphorylation) of the cAMP receptor. (c) The inhibitory effects of GTP gamma S and GDP beta S on cAMP binding are reduced; the stimulatory effect of cAMP on GTP gamma S binding is lost in fgd A mutants. (d) Basal high-affinity GTPase activity is reduced 40% and the stimulatory effect of cAMP is decreased from 40% in wild type to 30% in fgd A. (e) GTP-mediated stimulation and inhibition of adenylate cyclase is normal in mutant membranes. The results suggest a defective interaction between cell surface cAMP receptors and a specific G-protein in fgd A mutants. This interaction appears to be essential for nearly all signal transduction pathways in Dictyostelium discoideum.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Gao, T., Roisin-Bouffay, C., Hatton, R. D., Tang, L., Brock, D. A., DeShazo, T., Olson, L., Hong, W.-P., Jang, W., Canseco, E., Bakthavatsalam, D., Gomer, R. H. (2007). A Cell Number-Counting Factor Regulates Levels of a Novel Protein, SslA, as Part of a Group Size Regulation Mechanism in Dictyostelium. Eukaryot Cell 6: 1538-1551 [Abstract] [Full Text]
Mahadeo, D. C., Janka-Junttila, M., Smoot, R. L., Roselova, P., Parent, C. A. (2007). A Chemoattractant-mediated Gi-coupled Pathway Activates Adenylyl Cyclase in Human Neutrophils. Mol. Biol. Cell 18: 512-522 [Abstract] [Full Text]
Deery, W. J., Gao, T., Ammann, R., Gomer, R. H. (2002). A Single Cell Density-sensing Factor Stimulates Distinct Signal Transduction Pathways through Two Different Receptors. J. Biol. Chem. 277: 31972-31979 [Abstract] [Full Text]
Parent, C. A., Devreotes, P. N. (1999). A Cell's Sense of Direction. Science 284: 765-770 [Abstract] [Full Text]
Laub, M. T., Loomis, W. F. (1998). A Molecular Network That Produces Spontaneous Oscillations in Excitable Cells of Dictyostelium. Mol. Biol. Cell 9: 3521-3532 [Abstract] [Full Text]
Lindsey, D. F., Amerik, A., Deery, W. J., Bishop, J. D., Hochstrasser, M., Gomer, R. H. (1998). A Deubiquitinating Enzyme That Disassembles Free Polyubiquitin Chains Is Required for Development but Not Growth in Dictyostelium. J. Biol. Chem. 273: 29178-29187 [Abstract] [Full Text]
Jin, T., Amzel, M., Devreotes, P. N., Wu, L. (1998). Selection of Gbeta Subunits with Point Mutations That Fail to Activate Specific Signaling Pathways In Vivo: Dissecting Cellular Responses Mediated by a Heterotrimeric G Protein in Dictyostelium discoideum. Mol. Biol. Cell 9: 2949-2961 [Abstract] [Full Text]
Brazill, D. T., Lindsey, D. F., Bishop, J. D., Gomer, R. H. (1998). Cell Density Sensing Mediated by a G Protein-coupled Receptor Activating Phospholipase C. J. Biol. Chem. 273: 8161-8168 [Abstract] [Full Text]
Chen, M.-Y., Long, Y., Devreotes, P. N. (1997). A novel cytosolic regulator, Pianissimo, is required for chemoattractant receptor and G protein-mediated activation of the 12�transmembrane domain adenylyl cyclase in�Dictyostelium. Genes Dev. 11: 3218-3231 [Abstract] [Full Text]
Milne, J. L.S., Caterina, M. J., Devreotes, P. N. (1997). Random Mutagenesis of the cAMP Chemoattractant Receptor, cAR1, of Dictyostelium. EVIDENCE FOR MULTIPLE STATES OF ACTIVATION. J. Biol. Chem. 272: 2069-2076 [Abstract] [Full Text]
Firtel, R A (1995). Integration of signaling information in controlling cell-fate decisions in Dictyostelium.. Genes Dev. 9: 1427-1444
Caterina, M. J., Hereld, D., Devreotes, P. N. (1995). Occupancy of the Dictyostelium cAMP Receptor, cAR1, Induces a Reduction in Affinity Which Depends upon COOH-terminal Serine Residues. J. Biol. Chem. 270: 4418-4423 [Abstract] [Full Text]
Dharmawardhane, S, Cubitt, A., Clark, A., Firtel, R. (1994). Regulatory role of the G alpha 1 subunit in controlling cellular morphogenesis in Dictyostelium. Development 120: 3549-3561 [Abstract]
Lilly, P, Wu, L, Welker, D L, Devreotes, P N (1993). A G-protein beta-subunit is essential for Dictyostelium development.. Genes Dev. 7: 986-995 [Abstract]
Hart, A C, Kramer, H, Van Vactor, D L, Paidhungat, M, Zipursky, S L (1990). Induction of cell fate in the Drosophila retina: the bride of sevenless protein is predicted to contain a large extracellular domain and seven transmembrane segments.. Genes Dev. 4: 1835-1847 [Abstract]
Gundersen, R., Devreotes, P. (1990). In vivo receptor-mediated phosphorylation of a G protein in Dictyostelium. Science 248: 591-593 [Abstract]
Devreotes, P (1989). Dictyostelium discoideum: a model system for cell-cell interactions in development. Science 245: 1054-1058 [Abstract]
Kumagai, A., Mann, S.K.O., Pupillo, M., Pitt, G., Devreotes, P.N., Firtel, R.A. (1988). A Molecular Analysis of G Proteins and Control of Early Gene Expression by the Cell-surface cAMP Receptor in Dictyostelium. Cold Spring Harb Symp Quant Biol 53: 675-685 [Abstract]
Tang, L., Ammann, R., Gao, T., Gomer, R. H. (2001). A Cell Number-counting Factor Regulates Group Size in Dictyostelium by Differentially Modulating cAMP-induced cAMP and cGMP Pulse Sizes. J. Biol. Chem. 276: 27663-27669 [Abstract] [Full Text]
Roelofs, J., Loovers, H. M., Van Haastert, P. J. M. (2001). GTPgamma S Regulation of a 12-Transmembrane Guanylyl Cyclase Is Retained after Mutation to an Adenylyl Cyclase. J. Biol. Chem. 276: 40740-40745 [Abstract] [Full Text]