The centrin-based cytoskeleton of Chlamydomonas reinhardtii: distribution in interphase and mitotic cells

doi:10.1083/jcb.107.2.635

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The centrin-based cytoskeleton of Chlamydomonas reinhardtii: distribution in interphase and mitotic cells

JL Salisbury, AT Baron and MA Sanders
Center for NeuroSciences, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.

Monoclonal and polyclonal antibodies raised against algal centrin, a protein of algal striated flagellar roots, were used to characterize the occurrence and distribution of this protein in interphase and mitotic Chlamydomonas cells. Chlamydomonas centrin, as identified by Western immunoblot procedures, is a low molecular (20,000-Mr) acidic protein. Immunofluorescence and immunogold labeling demonstrates that centrin is a component of the distal fiber. In addition, centrin-based flagellar roots link the flagellar apparatus to the nucleus. Two major descending fibers extend from the basal bodies toward the nucleus; each descending fiber branches several times giving rise to 8-16 fimbria which surround and embrace the nucleus. Immunogold labeling indicates that these fimbria are juxtaposed to the outer nuclear envelope. Earlier studies have demonstrated that the centrin-based linkage between the flagellar apparatus and the nucleus is contractile, both in vitro and in living Chlamydomonas cells (Wright, R. L., J. Salisbury, and J. Jarvik. 1985. J. Cell Biol. 101:1903-1912; Salisbury, J. L., M. A. Sanders, and L. Harpst. 1987. J. Cell Biol. 105:1799-1805). Immunofluorescence studies show dramatic changes in distribution of the centrin-based system during mitosis that include a transient contraction at preprophase; division, separation, and re-extension during prophase; and a second transient contraction at the metaphase/anaphase boundary. These observations suggest a fundamental role for centrin in motile events during mitosis.
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LeDizet, M., Beck, J. C., Finkbeiner, W. E. (1998). Differential regulation of centrin genes during ciliogenesis in human tracheal epithelial cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 275: L1145-L1156 [Abstract] [Full Text]
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Finst, R. J., Kim, P. J., Quarmby, L. M. (1998). Genetics of the Deflagellation Pathway in Chlamydomonas. Genetics 149: 927-936 [Abstract] [Full Text]
Wilson, N. F., Foglesong, M. J., Snell, W. J. (1997). The Chlamydomonas Mating Type Plus Fertilization Tubule, a Prototypic Cell Fusion Organelle: Isolation, Characterization, and In Vitro Adhesion to Mating Type Minus Gametes. JCB 137: 1537-1553 [Abstract] [Full Text]
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Perret, E, Moudjou, M, Geraud, M., Derancourt, J, Soyer-Gobillard, M., Bornens, M (1995). Identification of an HSP70-related protein associated with the centrosome from dinoflagellates to human cells. J. Cell Sci. 108: 711-725 [Abstract]
Baron, A., Suman, V., Nemeth, E, Salisbury, J. (1994). The pericentriolar lattice of PtK2 cells exhibits temperature and calcium-modulated behavior. J. Cell Sci. 107: 2993-3003 [Abstract]
Errabolu, R, Sanders, M., Salisbury, J. (1994). Cloning of a cDNA encoding human centrin, an EF-hand protein of centrosomes and mitotic spindle poles. J. Cell Sci. 107: 9-16 [Abstract]
Kagami, O., Kamiya, R. (1992). Translocation and rotation of microtubules caused by multiple species of Chlamydomonas inner-arm dynein. J. Cell Sci. 103: 653-664 [Abstract]
Araki, M., Masutani, C., Takemura, M., Uchida, A., Sugasawa, K., Kondoh, J., Ohkuma, Y., Hanaoka, F. (2001). Centrosome Protein Centrin 2/Caltractin 1 Is Part of the Xeroderma Pigmentosum Group C Complex That Initiates Global Genome Nucleotide Excision Repair. J. Biol. Chem. 276: 18665-18672 [Abstract] [Full Text]