Focal expression of insulin-like growth factor I in rat kidney collecting duct

doi:10.1083/jcb.107.2.811

This Article

	Full Text (PDF, 7914K)
	Alert me when this article is cited

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Bortz, J. D.
	Articles by Hammerman, M. R.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Bortz, J. D.
	Articles by Hammerman, M. R.


Social Bookmarking

	What's this?

ARTICLES

Focal expression of insulin-like growth factor I in rat kidney collecting duct

JD Bortz, P Rotwein, D DeVol, PJ Bechtel, VA Hansen and MR Hammerman
Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.

To address the question of insulin-like growth factor (IGF) I localization and synthesis in kidney, we used two complementary experimental approaches: immunohistochemistry of fixed paraffin- embedded rat kidney sections; and measurement of IGF I mRNA in isolated components of the rat nephron, using a highly sensitive and specific solution hybridization assay. Immunostainable IGF I was localized exclusively to principal cells of cortical and medullary collecting ducts. Administration of growth hormone to hypophysectomized rats for 8 d resulted in enhanced immunohistochemical staining of IGF I within collecting ducts, but no detectable IGF I in other portions of the nephron. The abundance of IGF I mRNA was 7-12-fold higher in isolated papillary collecting ducts than in proximal tubules or glomeruli, and was enriched 10-fold compared with whole kidney. Our data demonstrate colocalization of IGF I and IGF I mRNA in the collecting duct, consistent with focal expression of the IGF I gene at this site.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Dimke, H., Flyvbjerg, A., Bourgeois, S., Thomsen, K., Frokiaer, J., Houillier, P., Nielsen, S., Frische, S. (2007). Acute growth hormone administration induces antidiuretic and antinatriuretic effects and increases phosphorylation of NKCC2. Am. J. Physiol. Renal Physiol. 292: F723-F735 [Abstract] [Full Text]
Shinada, M., Akdeniz, A., Panagiotopoulos, S., Jerums, G., Bach, L. A. (2000). Proteolysis of Insulin-Like Growth Factor-Binding Protein-3 Is Increased in Urine from Patients with Diabetic Nephropathy. J. Clin. Endocrinol. Metab. 85: 1163-1169 [Abstract] [Full Text]
Haylor, J. L., Mckillop, I. H., Oldroyd, S. D., El Nahas, M. A. (2000). IGF-I inhibitors reduce compensatory hyperfiltration in the isolated rat kidney following unilateral nephrectomy. Nephrol Dial Transplant 15: 87-92 [Abstract] [Full Text]
Piron, A., Leonard, I., Nonclercq, D., Toubeau, G., Falmagne, P., Heuson-Stiennon, J.-A., Laurent, G. (1998). In vitro demonstration of a mitogenic activity in renal tissue extracts during regenerative hyperplasia. Am. J. Physiol. Renal Physiol. 274: F348-F357 [Abstract] [Full Text]