Expression of apolipoprotein E by cultured vascular smooth muscle cells is controlled by growth state

doi:10.1083/jcb.107.3.1207

This Article

	Full Text (PDF, 1445K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Majack, R. A.
	Articles by Gebicke-Haerter, P. J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Majack, R. A.
	Articles by Gebicke-Haerter, P. J.


Social Bookmarking

	What's this?

ARTICLES

Expression of apolipoprotein E by cultured vascular smooth muscle cells is controlled by growth state

RA Majack, CK Castle, LV Goodman, KH Weisgraber, RW Mahley, EM Shooter and PJ Gebicke-Haerter
Atherosclerosis and Thrombosis Research, Upjohn Company, Kalamazoo, Michigan 49001.

Rat vascular smooth muscle cells (SMC) in culture synthesize and secrete a approximately 38,000-Mr protein doublet or triplet that, as previously described (Majack and Bornstein. 1984. J. Cell Biol. 99:1688- 1695), rapidly and reversibly accumulates in the SMC culture medium upon addition of heparin. In the present study, we show that this approximately 38,000-Mr heparin-regulated protein is electrophoretically and immunologically identical to apolipoprotein E (apo-E), a major plasma apolipoprotein involved in cholesterol transport. In addition, we show that expression of apo-E by cultured SMC varies according to growth state: while proliferating SMC produced little apo-E and expressed low levels of apo-E mRNA, quiescent SMC produced significantly more apo-E (relative to other proteins) and expressed markedly increased levels of apo-E mRNA. Northern analysis of RNA extracted from aortic tissue revealed that fully differentiated, quiescent SMC contain significant quantities of apo-E mRNA. These data establish aortic SMC as a vascular source for apo-E and suggest new functional roles for this apolipoprotein, possibly unrelated to traditional concepts of lipid metabolism.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Mayr, M., Zampetaki, A., Sidibe, A., Mayr, U., Yin, X., De Souza, A. I., Chung, Y.-L., Madhu, B., Quax, P. H., Hu, Y., Griffiths, J. R., Xu, Q. (2008). Proteomic and Metabolomic Analysis of Smooth Muscle Cells Derived From the Arterial Media and Adventitial Progenitors of Apolipoprotein E-Deficient Mice. Circ. Res. 102: 1046-1056 [Abstract] [Full Text]
Friedman, S. L. (2008). Hepatic Stellate Cells: Protean, Multifunctional, and Enigmatic Cells of the Liver. Physiol. Rev. 88: 125-172 [Abstract] [Full Text]
Xu, Q., Bernardo, A., Walker, D., Kanegawa, T., Mahley, R. W., Huang, Y. (2006). Profile and Regulation of Apolipoprotein E (ApoE) Expression in the CNS in Mice with Targeting of Green Fluorescent Protein Gene to the ApoE Locus.. J. Neurosci. 26: 4985-4994 [Abstract] [Full Text]
Moore, Z. W.Q., Zhu, B., Kuhel, D. G., Hui, D. Y. (2004). Vascular Apolipoprotein E Expression and Recruitment from Circulation to Modulate Smooth Muscle Cell Response to Endothelial Denudation. Am. J. Pathol. 164: 2109-2116 [Abstract] [Full Text]
Burgos, J. S., Ramirez, C., Sastre, I., Bullido, M. J., Valdivieso, F. (2002). Involvement of Apolipoprotein E in the Hematogenous Route of Herpes Simplex Virus Type 1 to the Central Nervous System. J. Virol. 76: 12394-12398 [Abstract] [Full Text]
Ho, Y.-Y., Deckelbaum, R. J., Chen, Y., Vogel, T., Talmage, D. A. (2001). Apolipoprotein E Inhibits Serum-stimulated Cell Proliferation and Enhances Serum-independent Cell Proliferation. J. Biol. Chem. 276: 43455-43462 [Abstract] [Full Text]
Paka, L., Goldberg, I. J., Obunike, J. C., Choi, S. Y., Saxena, U., Goldberg, I. D., Pillarisetti, S. (1999). Perlecan Mediates the Antiproliferative Effect of Apolipoprotein E on Smooth Muscle Cells. AN UNDERLYING MECHANISM FOR THE MODULATION OF SMOOTH MUSCLE CELL GROWTH?. J. Biol. Chem. 274: 36403-36408 [Abstract] [Full Text]
Ishigami, M., Swertfeger, D. K., Granholm, N. A., Hui, D. Y. (1998). Apolipoprotein E Inhibits Platelet-derived Growth Factor-induced Vascular Smooth Muscle Cell Migration and Proliferation by Suppressing Signal Transduction and Preventing Cell Entry to G1 Phase. J. Biol. Chem. 273: 20156-20161 [Abstract] [Full Text]