Human immunodeficiency virus infection of T cells and monocytes proceeds via receptor-mediated endocytosis

doi:10.1083/jcb.107.3.959

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Human immunodeficiency virus infection of T cells and monocytes proceeds via receptor-mediated endocytosis

CD Pauza and TM Price
Developmental Biology Laboratory, Salk Institute for Biological Studies, San Diego, California 92138.

The rates of internalization and uncoating of 32P-labelled human immunodeficiency virus (HIV) in the human T lymphoid cell line CEM are consonant with a receptor-mediated endocytosis mechanism of entry. This interpretation was affirmed by electron microscopic observation of virions within endosomes. Virus binding and infectivity were inhibited to the same extent by pretreatment with OKT4A antibody, therefore, the CD4 receptor-dependent pathway of internalization appears to be the infectious route of entry. The pattern of internalization by the human monoblastoid cell line U937 proved to be more complex, involving rapid and efficient CD4-independent internalization. Electron microscopy revealed the presence of large intracellular vesicles, each containing several virions. Antibody against the CD4 receptor for virus efficiently blocked infection, but did not reduce significantly HIV binding or internalization in the U937 cell line. Consequently, U937 cells have a CD4-independent pathway of virus internalization that does not coincide with the route of entry for infectious HIV.
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Frank, I., Piatak, M. Jr., Stoessel, H., Romani, N., Bonnyay, D., Lifson, J.D., Pope, M. (2002). Infectious and Whole Inactivated Simian Immunodeficiency Viruses Interact Similarly with Primate Dendritic Cells (DCs): Differential Intracellular Fate of Virions in Mature and Immature DCs. J. Virol. 76: 2936-2951 [Abstract] [Full Text]
Marechal, V., Prevost, M.-C., Petit, C., Perret, E., Heard, J.-M., Schwartz, O. (2001). Human Immunodeficiency Virus Type 1 Entry into Macrophages Mediated by Macropinocytosis. J. Virol. 75: 11166-11177 [Abstract] [Full Text]
Chazal, N., Singer, G., Aiken, C., Hammarskjöld, M.-L., Rekosh, D. (2001). Human Immunodeficiency Virus Type 1 Particles Pseudotyped with Envelope Proteins That Fuse at Low pH No Longer Require Nef for Optimal Infectivity. J. Virol. 75: 4014-4018 [Abstract] [Full Text]
Takikawa, S., Ishii, K., Aizaki, H., Suzuki, T., Asakura, H., Matsuura, Y., Miyamura, T. (2000). Cell Fusion Activity of Hepatitis C Virus Envelope Proteins. J. Virol. 74: 5066-5074 [Abstract] [Full Text]
Boritz, E., Gerlach, J., Johnson, J. E., Rose, J. K. (1999). Replication-Competent Rhabdoviruses with Human Immunodeficiency Virus Type 1 Coats and Green Fluorescent Protein: Entry by a pH-Independent Pathway. J. Virol. 73: 6937-6945 [Abstract] [Full Text]
Marianneau, P., Steffan, A.-M., Royer, C., Drouet, M.-T., Jaeck, D., Kirn, A., Deubel, V. (1999). Infection of Primary Cultures of Human Kupffer Cells by Dengue Virus: No Viral Progeny Synthesis, but Cytokine Production Is Evident. J. Virol. 73: 5201-5206 [Abstract] [Full Text]
Montaner, L. J., da Silva, R. P., Sun, J., Sutterwala, S., Hollinshead, M., Vaux, D., Gordon, S. (1999). Type 1 and Type 2 Cytokine Regulation of Macrophage Endocytosis: Differential Activation by IL-4/IL-13 as Opposed to IFN-{gamma} or IL-10. J. Immunol. 162: 4606-4613 [Abstract] [Full Text]
Marechal, V., Clavel, F., Heard, J. M., Schwartz, O. (1998). Cytosolic Gag p24 as an Index of Productive Entry of Human Immunodeficiency Virus Type 1. J. Virol. 72: 2208-2212 [Abstract] [Full Text]
Granelli-Piperno, A., Moser, B., Pope, M., Chen, D., Wei, Y., Isdell, F., O'Doherty, U., Paxton, W., Koup, R., Mojsov, S., Bhardwaj, N., Clark-Lewis, I., Baggiolini, M., Steinman, R. M. (1996). Efficient Interaction of HIV-1 with Purified Dendritic Cells via Multiple Chemokine Coreceptors. JEM 184: 2433-2438 [Abstract] [Full Text]
Simm, M., Pekarskaya, O., Volsky, D. J. (1996). Synthesis of Full-length Viral DNA in CD4-positive Membrane Vesicles Exposed to HIV-1. A MODEL FOR STUDIES OF EARLY STAGES OF THE HIV-1 LIFE CYCLE. J. Biol. Chem. 271: 28266-28270 [Abstract] [Full Text]
Hwang, S., Boyle, T., Lyerly, H., Cullen, B. (1991). Identification of the envelope V3 loop as the primary determinant of cell tropism in HIV-1. Science 253: 71-74 [Abstract]
Levy, J. A. (1989). Human Immunodeficiency Viruses and the Pathogenesis of AIDS. JAMA 261: 2997-3006 [Abstract]