Actions of cytochalasins on the organization of actin filaments and microtubules in a neuronal growth cone

doi:10.1083/jcb.107.4.1505

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Actions of cytochalasins on the organization of actin filaments and microtubules in a neuronal growth cone

P Forscher and SJ Smith
Howard Hughes Medical Institute Research Laboratories, Yale University School of Medicine, Section of Molecular Neurobiology, New Haven, Connecticut 06510.

Actions of cytochalasin B (CB) on cytoskeletons and motility of growth cones from cultured Aplysia neurons were studied using a rapid flow perfusion chamber and digital video light microscopy. Living growth cones were observed using differential interference contrast optics and were also fixed at various time points to assay actin filament (F- actin) and microtubule distributions. Treatment with CB reversibly blocked motility and eliminated most of the phalloidin-stainable F- actin from the leading lamella. The loss of F-actin was nearly complete within 2-3 min of CB application and was largely reversed within 5-6 min of CB removal. The loss and recovery of F-actin were found to occur with a very distinctive spatial organization. Within 20-30 s of CB application, F-actin networks receded from the entire peripheral margin of the lamella forming a band devoid of F-actin. This band widened as F- actin receded at rates of 3-6 microns/min. Upon removal of CB, F-actin began to reappear within 20-30 s. The initial reappearance of F-actin took two forms: a coarse isotropic matrix of F-actin bundles throughout the lamella, and a denser matrix along the peripheral margin. The denser peripheral matrix then expanded in width, extending centrally to replace the coarse matrix at rates again between 3-6 microns/min. These results suggest that actin normally polymerizes at the leading edge and then flows rearward at a rate between 3-6 microns/min. CB treatment was also observed to alter the distribution of microtubules, assayed by antitubulin antibody staining. Normally, microtubules are restricted to the neurite shaft and a central growth cone domain. Within approximately 5 min after CB application, however, microtubules began extending into the lamellar region, often reaching the peripheral margin. Upon removal of CB, the microtubules were restored to their former central localization. The timing of these microtubule redistributions is consistent with their being secondary to effects of CB on lamellar F-actin.
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Yoshimura, T., Arimura, N., Kaibuchi, K. (2006). Signaling networks in neuronal polarization.. J. Neurosci. 26: 10626-10630 [Abstract] [Full Text]
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Tilney, L. G., DeRosier, D. J. (2005). Inaugural Article: How to make a curved Drosophila bristle using straight actin bundles. Proc. Natl. Acad. Sci. USA 102: 18785-18792 [Abstract] [Full Text]
Weisswange, I., Bretschneider, T., Anderson, K. I. (2005). The leading edge is a lipid diffusion barrier. J. Cell Sci. 118: 4375-4380 [Abstract] [Full Text]
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Lehmann, M. J., Sherer, N. M., Marks, C. B., Pypaert, M., Mothes, W. (2005). Actin- and myosin-driven movement of viruses along filopodia precedes their entry into cells. JCB 170: 317-325 [Abstract] [Full Text]
Tani, T., Miyamoto, Y., Fujimori, K. E., Taguchi, T., Yanagida, T., Sako, Y., Harada, Y. (2005). Trafficking of a Ligand-Receptor Complex on the Growth Cones as an Essential Step for the Uptake of Nerve Growth Factor at the Distal End of the Axon: A Single-Molecule Analysis. J. Neurosci. 25: 2181-2191 [Abstract] [Full Text]
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Hotta, A., Inatome, R., Yuasa-Kawada, J., Qin, Q., Yamamura, H., Yanagi, S. (2005). Critical Role of Collapsin Response Mediator Protein-associated Molecule CRAM for Filopodia and Growth Cone Development in Neurons. Mol. Biol. Cell 16: 32-39 [Abstract] [Full Text]
Ponti, A., Machacek, M., Gupton, S. L., Waterman-Storer, C. M., Danuser, G. (2004). Two Distinct Actin Networks Drive the Protrusion of Migrating Cells. Science 305: 1782-1786 [Abstract] [Full Text]
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Suh, L. H., Oster, S. F., Soehrman, S. S., Grenningloh, G., Sretavan, D. W. (2004). L1/Laminin Modulation of Growth Cone Response to EphB Triggers Growth Pauses and Regulates the Microtubule Destabilizing Protein SCG10. J. Neurosci. 24: 1976-1986 [Abstract] [Full Text]
Tanaka, J., Yan, Y., Choi, J., Bai, J., Klenchin, V. A., Rayment, I., Marriott, G. (2003). Biomolecular mimicry in the actin cytoskeleton: Mechanisms underlying the cytotoxicity of kabiramide C and related macrolides. Proc. Natl. Acad. Sci. USA 100: 13851-13856 [Abstract] [Full Text]
Giustetto, M., Hegde, A. N., Si, K., Casadio, A., Inokuchi, K., Pei, W., Kandel, E. R., Schwartz, J. H. (2003). Axonal transport of eukaryotic translation elongation factor 1{alpha} mRNA couples transcription in the nucleus to long-term facilitation at the synapse. Proc. Natl. Acad. Sci. USA 100: 13680-13685 [Abstract] [Full Text]
Grabham, P. W., Reznik, B., Goldberg, D. J. (2003). Microtubule and Rac 1-dependent F-actin in growth cones. J. Cell Sci. 116: 3739-3748 [Abstract] [Full Text]
Goldberg, J. L. (2003). How does an axon grow?. Genes Dev. 17: 941-958 [Full Text]
Bamburg, J. R. (2003). Introduction to Cytoskeletal Dynamics and Pathfinding of Neuronal Growth Cones. J. Histochem. Cytochem. 51: 407-409 [Abstract] [Full Text]
Gungabissoon, R. A., Bamburg, J. R. (2003). Regulation of Growth Cone Actin Dynamics by ADF/Cofilin. J. Histochem. Cytochem. 51: 411-420 [Abstract] [Full Text]
Legg, A. T., O'Connor, T. P. (2003). Gradients and Growth Cone Guidance of Grasshopper Neurons. J. Histochem. Cytochem. 51: 445-454 [Abstract] [Full Text]
Brown, M. E., Bridgman, P. C. (2003). Retrograde flow rate is increased in growth cones from myosin IIB knockout mice. J. Cell Sci. 116: 1087-1094 [Abstract] [Full Text]
Bernstein, B. W., Bamburg, J. R. (2003). Actin-ATP Hydrolysis Is a Major Energy Drain for Neurons. J. Neurosci. 23: 1-6 [Abstract] [Full Text]
Ji, J.-Y., Haghnia, M., Trusty, C., Goldstein, L. S. B., Schubiger, G. (2002). A Genetic Screen for Suppressors and Enhancers of the Drosophila Cdk1-Cyclin B Identifies Maternal Factors That Regulate Microtubule and Microfilament Stability. Genetics 162: 1179-1195 [Abstract] [Full Text]
Nakhost, A., Kabir, N., Forscher, P., Sossin, W. S. (2002). Protein Kinase C Isoforms Are Translocated to Microtubules in Neurons. J. Biol. Chem. 277: 40633-40639 [Abstract] [Full Text]
Viard, M., Parolini, I., Sargiacomo, M., Fecchi, K., Ramoni, C., Ablan, S., Ruscetti, F. W., Wang, J. M., Blumenthal, R. (2002). Role of Cholesterol in Human Immunodeficiency Virus Type 1 Envelope Protein-Mediated Fusion with Host Cells. J. Virol. 76: 11584-11595 [Abstract] [Full Text]
Diefenbach, T. J., Latham, V. M., Yimlamai, D., Liu, C. A., Herman, I. M., Jay, D. G. (2002). Myosin 1c and myosin IIB serve opposing roles in lamellipodial dynamics of the neuronal growth cone. JCB 158: 1207-1217 [Abstract] [Full Text]
Schaefer, A. W., Kabir, N., Forscher, P. (2002). Filopodia and actin arcs guide the assembly and transport of two populations of microtubules with unique dynamic parameters in neuronal growth cones. JCB 158: 139-152 [Abstract] [Full Text]
Yvon, A.-M. C., Walker, J. W., Danowski, B., Fagerstrom, C., Khodjakov, A., Wadsworth, P. (2002). Centrosome Reorientation in Wound-Edge Cells Is Cell Type Specific. Mol. Biol. Cell 13: 1871-1880 [Abstract] [Full Text]
Zhou, F.-Q., Waterman-Storer, C. M., Cohan, C. S. (2002). Focal loss of actin bundles causes microtubule redistribution and growth cone turning. JCB 157: 839-849 [Abstract] [Full Text]
Henson, J. H., Nazarian, R., Schulberg, K. L., Trabosh, V. A., Kolnik, S. E., Burns, A. R., McPartland, K. J. (2002). Wound Closure in the Lamellipodia of Single Cells: Mediation by Actin Polymerization in the Absence of an Actomyosin Purse String. Mol. Biol. Cell 13: 1001-1014 [Abstract] [Full Text]
Scalettar, B. A., Rosa, P., Taverna, E., Francolini, M., Tsuboi, T., Terakawa, S., Koizumi, S., Roder, J., Jeromin, A. (2002). Neuronal calcium sensor-1 binds to regulated secretory organelles and functions in basal and stimulated exocytosis in PC12 cells. J. Cell Sci. 115: 2399-2412 [Abstract] [Full Text]
Lee, S., Kolodziej, P. A. (2002). Short Stop provides an essential link between F-actin and microtubules during axon extension. Development 129: 1195-1204 [Abstract] [Full Text]
Dent, E. W., Kalil, K. (2001). Axon Branching Requires Interactions between Dynamic Microtubules and Actin Filaments. J. Neurosci. 21: 9757-9769 [Abstract] [Full Text]
Kamiguchi, H., Yoshihara, F. (2001). The Role of Endocytic L1 Trafficking in Polarized Adhesion and Migration of Nerve Growth Cones. J. Neurosci. 21: 9194-9203 [Abstract] [Full Text]
Suter, D. M., Forscher, P. (2001). Transmission of growth cone traction force through apCAM-cytoskeletal linkages is regulated by Src family tyrosine kinase activity. JCB 155: 427-438 [Abstract] [Full Text]
Berg, A., Rubin, K., Reed, R. K. (2001). Cytochalasin D induces edema formation and lowering of interstitial fluid pressure in rat dermis. Am. J. Physiol. Heart Circ. Physiol. 281: H7-H13 [Abstract] [Full Text]
Zhou, F.-q., Cohan, C. S. (2001). Growth Cone Collapse through Coincident Loss of Actin Bundles and Leading Edge Actin without Actin Depolymerization. JCB 153: 1071-1084 [Abstract] [Full Text]
Kunda, P., Paglini, G., Quiroga, S., Kosik, K., Caceres, A. (2001). Evidence for the Involvement of Tiam1 in Axon Formation. J. Neurosci. 21: 2361-2372 [Abstract] [Full Text]
Kabir, N., Schaefer, A. W., Nakhost, A., Sossin, W. S., Forscher, P. (2001). Protein Kinase C Activation Promotes Microtubule Advance in Neuronal Growth Cones by Increasing Average Microtubule Growth Lifetimes. JCB 152: 1033-1044 [Abstract] [Full Text]
Gorodeski, G. I. (2000). cGMP-dependent ADP depolymerization of actin mediates estrogen increase in cervical epithelial permeability. Am. J. Physiol. Cell Physiol. 279: C2028-C2036 [Abstract] [Full Text]
Tian, L., Nyman, H., Kilgannon, P., Yoshihara, Y., Mori, K., Andersson, L. C., Kaukinen, S., Rauvala, H., Gallatin, W. M., Gahmberg, C. G. (2000). Intercellular Adhesion Molecule-5 Induces Dendritic Outgrowth by Homophilic Adhesion. JCB 150: 243-252 [Abstract] [Full Text]
Frey, D., Laux, T., Xu, L., Schneider, C., Caroni, P. (2000). Shared and Unique Roles of Cap23 and Gap43 in Actin Regulation, Neurite Outgrowth, and Anatomical Plasticity. JCB 149: 1443-1454 [Abstract] [Full Text]
Kamiguchi, H., Lemmon, V. (2000). Recycling of the Cell Adhesion Molecule L1 in Axonal Growth Cones. J. Neurosci. 20: 3676-3686 [Abstract] [Full Text]
Roberts, T. M., Stewart, M. (2000). Acting like Actin: The Dynamics of the Nematode Major Sperm Protein (Msp) Cytoskeleton Indicate a Push-Pull Mechanism for Amoeboid Cell Motility. JCB 149: 7-12 [Full Text]
Meberg, P. J., Bamburg, J. R. (2000). Increase in Neurite Outgrowth Mediated by Overexpression of Actin Depolymerizing Factor. J. Neurosci. 20: 2459-2469 [Abstract] [Full Text]
Ruthel, G., Hollenbeck, P. J. (2000). Growth Cones Are Not Required for Initial Establishment of Polarity or Differential Axon Branch Growth in Cultured Hippocampal Neurons. J. Neurosci. 20: 2266-2274 [Abstract] [Full Text]
Nishizaka, T., Shi, Q., Sheetz, M. P. (2000). Position-dependent linkages of fibronectin- integrin-cytoskeleton. Proc. Natl. Acad. Sci. USA 97: 692-697 [Abstract] [Full Text]
Grabham, P., Foley, M, Umeojiako, A, Goldberg, D. (2000). Nerve growth factor stimulates coupling of beta1 integrin to distinct transport mechanisms in the filopodia of growth cones. J. Cell Sci. 113: 3003-3012 [Abstract]
Henson, J. H., Svitkina, T. M., Burns, A. R., Hughes, H. E., MacPartland, K. J., Nazarian, R., Borisy, G. G. (1999). Two Components of Actin-based Retrograde Flow in Sea Urchin Coelomocytes. Mol. Biol. Cell 10: 4075-4090 [Abstract] [Full Text]
Burton, K., Park, J. H., Taylor, D. L. (1999). Keratocytes Generate Traction Forces in Two Phases. Mol. Biol. Cell 10: 3745-3769 [Abstract] [Full Text]
Welnhofer, E. A., Zhao, L., Cohan, C. S. (1999). Calcium Influx Alters Actin Bundle Dynamics and Retrograde Flow in Helisoma Growth Cones. J. Neurosci. 19: 7971-7982 [Abstract] [Full Text]
Italiano, J. E. Jr., Stewart, M., Roberts, T. M. (1999). Localized Depolymerization of the Major Sperm Protein Cytoskeleton Correlates with the Forward Movement of the Cell Body in the Amoeboid Movement of Nematode Sperm. JCB 146: 1087-1096 [Abstract] [Full Text]
Mallavarapu, A., Mitchison, T. (1999). Regulated Actin Cytoskeleton Assembly at Filopodium Tips Controls Their Extension and Retraction. JCB 146: 1097-1106 [Abstract] [Full Text]
Katoh, K., Hammar, K., Smith, P. J. S., Oldenbourg, R. (1999). Arrangement of radial actin bundles in the growth cone of Aplysia bag cell neurons shows the immediate past history of filopodial behavior. Proc. Natl. Acad. Sci. USA 96: 7928-7931 [Abstract] [Full Text]
Rochlin, M. W., Dailey, M. E., Bridgman, P. C. (1999). Polymerizing Microtubules Activate Site-directed F-Actin Assembly in Nerve Growth Cones. Mol. Biol. Cell 10: 2309-2327 [Abstract] [Full Text]
Gallo, G., Letourneau, P. C. (1999). Different Contributions of Microtubule Dynamics and Transport to the Growth of Axons and Collateral Sprouts. J. Neurosci. 19: 3860-3873 [Abstract] [Full Text]
Isbister, C. M., O'Connor, T. P. (1999). Filopodial Adhesion Does Not Predict Growth Cone Steering Events In Vivo. J. Neurosci. 19: 2589-2600 [Abstract] [Full Text]
Bradke, F., Dotti, C. G. (1999). The Role of Local Actin Instability in Axon Formation. Science 283: 1931-1934 [Abstract] [Full Text]
Sider, J., Mandato, C., Weber, K., Zandy, A., Beach, D, Finst, R., Skoble, J, Bement, W. (1999). Direct observation of microtubule-f-actin interaction in cell free lysates. J. Cell Sci. 112: 1947-1956 [Abstract]
Fukui, Y, Kitanishi-Yumura, T, Yumura, S (1999). Myosin II-independent F-actin flow contributes to cell locomotion in dictyostelium. J. Cell Sci. 112: 877-886 [Abstract]
Katoh, K., Hammar, K., Smith, P. J.�S., Oldenbourg, R. (1999). Birefringence Imaging Directly Reveals Architectural Dynamics of Filamentous Actin in Living Growth Cones. Mol. Biol. Cell 10: 197-210 [Abstract] [Full Text]
Zakharenko, S., Popov, S. (1998). Dynamics of Axonal Microtubules Regulate the Topology of New Membrane Insertion into the Growing Neurites. JCB 143: 1077-1086 [Abstract] [Full Text]
Mullins, R. D., Heuser, J. A., Pollard, T. D. (1998). The interaction of Arp2/3 complex with actin: Nucleation, high affinity pointed end capping, and formation of branching networks of filaments. Proc. Natl. Acad. Sci. USA 95: 6181-6186 [Abstract] [Full Text]
Heidemann, S. R., Buxbaum, R. E. (1998). Cell Crawling: First the Motor, Now the Transmission. JCB 141: 1-4 [Full Text]
Suter, D. M., Errante, L. D., Belotserkovsky, V., Forscher, P. (1998). The Ig Superfamily Cell Adhesion Molecule, apCAM, Mediates Growth Cone Steering by Substrate-Cytoskeletal Coupling. JCB 141: 227-240 [Abstract] [Full Text]
Ballestrem, C, Wehrle-Haller, B, Imhof, B. (1998). Actin dynamics in living mammalian cells. J. Cell Sci. 111: 1649-1658 [Abstract]
Kita, Y, Kimura, K., Kobayashi, M, Ihara, S, Kaibuchi, K, Kuroda, S, Ui, M, Iba, H, Konishi, H, Kikkawa, U, Nagata, S, Fukui, Y (1998). Microinjection of activated phosphatidylinositol-3 kinase induces process outgrowth in rat PC12 cells through the Rac-JNK signal transduction pathway. J. Cell Sci. 111: 907-915 [Abstract]
Kaech, S., Fischer, M., Doll, T., Matus, A. (1997). Isoform Specificity in the Relationship of Actin to Dendritic Spines. J. Neurosci. 17: 9565-9572 [Abstract] [Full Text]
Nakanishi, H., Obaishi, H., Satoh, A., Wada, M., Mandai, K., Satoh, K., Nishioka, H., Matsuura, Y., Mizoguchi, A., Takai, Y. (1997). Neurabin: A Novel Neural Tissue-specific Actin Filament-binding Protein Involved in Neurite Formation. JCB 139: 951-961 [Abstract] [Full Text]
Waterman-Storer, C. M., Salmon, E.D. (1997). Actomyosin-based Retrograde Flow of Microtubules in the Lamella of Migrating Epithelial Cells Influences Microtubule Dynamic Instability and Turnover and Is Associated with Microtubule Breakage and Treadmilling. JCB 139: 417-434 [Abstract] [Full Text]
Zhang, J., Larsen, T. H., Lieberman, M. (1997). F-actin modulates swelling-activated chloride current in cultured chick cardiac myocytes. Am. J. Physiol. Cell Physiol. 273: C1215-C1224 [Abstract] [Full Text]
Lu, M., Witke, W., Kwiatkowski, D. J., Kosik, K. S. (1997). Delayed Retraction of Filopodia in Gelsolin Null Mice. JCB 138: 1279-1287 [Abstract] [Full Text]
Shorte, S. L. (1997). N-Methyl-D-Aspartate Evokes Rapid Net Depolymerization of Filamentous Actin in Cultured Rat Cerebellar Granule Cells. J. Neurophysiol. 78: 1135-1143 [Abstract] [Full Text]
Grabham, P. W., Goldberg, D. J. (1997). Nerve Growth Factor Stimulates the Accumulation of beta 1 Integrin at the Tips of Filopodia in the Growth Cones of Sympathetic Neurons. J. Neurosci. 17: 5455-5465 [Abstract] [Full Text]
Kobayashi, M., Nagata, S., Kita, Y., Nakatsu, N., Ihara, S., Kaibuchi, K., Kuroda, S., Ui, M., Iba, H., Konishi, H., Kikkawa, U., Saitoh, I., Fukui, Y. (1997). Expression of a Constitutively Active Phosphatidylinositol 3-Kinase Induces Process Formation in Rat PC12 Cells. USE OF Cre/loxP RECOMBINATION SYSTEM. J. Biol. Chem. 272: 16089-16092 [Abstract] [Full Text]
Challacombe, J. F., Snow, D. M., Letourneau, P. C. (1997). Dynamic Microtubule Ends Are Required for Growth Cone Turning to Avoid an Inhibitory Guidance Cue. J. Neurosci. 17: 3085-3095 [Abstract] [Full Text]
Cramer, L. P., Siebert, M., Mitchison, T. J. (1997). Identification of Novel Graded Polarity Actin Filament Bundles in Locomoting Heart Fibroblasts: Implications for the Generation of Motile Force. JCB 136: 1287-1305 [Abstract] [Full Text]
Jones, B D (1997). Host responses to pathogenic Salmonella infection.. Genes Dev. 11: 679-687
Cunningham, C. C., Leclerc, N., Flanagan, L. A., Lu, M., Janmey, P. A., Kosik, K. S. (1997). Microtubule-associated Protein 2c Reorganizes Both Microtubules and Microfilaments into Distinct Cytological Structures in an Actin-binding Protein-280-deficient Melanoma Cell Line. JCB 136: 845-857 [Abstract] [Full Text]
Mascotti, F., Caceres, A., Pfenninger, K. H., Quiroga, S. (1997). Expression and Distribution of IGF-1 Receptors Containing a beta -Subunit Variant (beta gc) in Developing Neurons. J. Neurosci. 17: 1447-1459 [Abstract] [Full Text]
Verkhovsky, A., Svitkina, T., Borisy, G. (1997). Polarity sorting of actin filaments in cytochalasin-treated fibroblasts. J. Cell Sci. 110: 1693-1704 [Abstract]
Golsteyn, R., Beckerle, M., Koay, T, Friederich, E (1997). Structural and functional similarities between the human cytoskeletal protein zyxin and the ActA protein of Listeria monocytogenes. J. Cell Sci. 110: 1893-1906 [Abstract]
van Weering, D. H.J., Bos, J. L. (1997). Glial Cell Line-derived Neurotrophic Factor Induces Ret-mediated Lamellipodia Formation. J. Biol. Chem. 272: 249-254 [Abstract] [Full Text]