Nuclear and cytoplasmic mitotic cycles continue in Drosophila embryos in which DNA synthesis is inhibited with aphidicolin

doi:10.1083/jcb.107.6.2009

This Article

	Full Text (PDF, 4406K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Raff, J. W.
	Articles by Glover, D. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Raff, J. W.
	Articles by Glover, D. M.


Social Bookmarking

	What's this?

ARTICLES

Nuclear and cytoplasmic mitotic cycles continue in Drosophila embryos in which DNA synthesis is inhibited with aphidicolin

JW Raff and DM Glover
Department of Biochemistry, Imperial College of Science and Technology, London, United Kingdom.

We have microinjected aphidicolin, a specific inhibitor of DNA polymerase alpha, into syncytial Drosophila embryos. This treatment inhibits DNA synthesis and, as a consequence, nuclear replication. We demonstrate that under these conditions several cycles of both centrosome replication and cortical budding continue, although the cycles have a longer periodicity than is normally found. As in uninjected embryos, when the cortical buds are present, the embryos have nuclei containing decondensed chromatin surrounded by nuclear membranes as judged by bright annular staining with an anti-lamin antibody. As the buds recede, the unreplicated chromatin condenses and lamin staining becomes weak and diffuse. Thus, both cytoplasmic and nuclear aspects of the mitotic cycle continue following the inhibition of DNA replication in the Drosophila embryo.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

McCleland, M. L., Shermoen, A. W., O'Farrell, P. H. (2009). DNA replication times the cell cycle and contributes to the mid-blastula transition in Drosophila embryos. JCB 187: 7-14 [Abstract] [Full Text]
Eisman, R. C., Phelps, M. A. S., Kaufman, T. C. (2009). Centrosomin: A Complex Mix of Long and Short Isoforms Is Required for Centrosome Function During Early Development in Drosophila melanogaster. Genetics 182: 979-997 [Abstract] [Full Text]
Gao, G., Bi, X., Chen, J., Srikanta, D., Rong, Y. S. (2009). Mre11-Rad50-Nbs complex is required to cap telomeres during Drosophila embryogenesis. Proc. Natl. Acad. Sci. USA 106: 10728-10733 [Abstract] [Full Text]
Zhang, G., Breuer, M., Forster, A., Egger-Adam, D., Wodarz, A. (2009). Mars, a Drosophila protein related to vertebrate HURP, is required for the attachment of centrosomes to the mitotic spindle during syncytial nuclear divisions. J. Cell Sci. 122: 535-545 [Abstract] [Full Text]
Merkle, J. A., Rickmyre, J. L., Garg, A., Loggins, E. B., Jodoin, J. N., Lee, E., Wu, L. P., Lee, L. A. (2009). no poles encodes a predicted E3 ubiquitin ligase required for early embryonic development of Drosophila. Development 136: 449-459 [Abstract] [Full Text]
Bothwell, J. H. F., Kisielewska, J., Genner, M. J., McAinsh, M. R., Brownlee, C. (2008). Ca2+ signals coordinate zygotic polarization and cell cycle progression in the brown alga Fucus serratus. Development 135: 2173-2181 [Abstract] [Full Text]
Brunk, K., Vernay, B., Griffith, E., Reynolds, N. L., Strutt, D., Ingham, P. W., Jackson, A. P. (2007). Microcephalin coordinates mitosis in the syncytial Drosophila embryo. J. Cell Sci. 120: 3578-3588 [Abstract] [Full Text]
Rickmyre, J. L., DasGupta, S., Ooi, D. L.-Y., Keel, J., Lee, E., Kirschner, M. W., Waddell, S., Lee, L. A. (2007). The Drosophila homolog of MCPH1, a human microcephaly gene, is required for genomic stability in the early embryo. J. Cell Sci. 120: 3565-3577 [Abstract] [Full Text]
Adiga, S. K., Toyoshima, M., Shimura, T., Takeda, J., Uematsu, N., Niwa, O. (2007). Delayed and stage specific phosphorylation of H2AX during preimplantation development of {gamma}-irradiated mouse embryos. Reproduction 133: 415-422 [Abstract] [Full Text]
Crest, J., Oxnard, N., Ji, J.-Y., Schubiger, G. (2007). Onset of the DNA Replication Checkpoint in the Early Drosophila Embryo. Genetics 175: 567-584 [Abstract] [Full Text]
Tram, U., Fredrick, K., Werren, J. H., Sullivan, W. (2006). Paternal chromosome segregation during the first mitotic division determines Wolbachia-induced cytoplasmic incompatibility phenotype. J. Cell Sci. 119: 3655-3663 [Abstract] [Full Text]
Perez-Mongiovi, D., Malmanche, N., Bousbaa, H., Sunkel, C. (2005). Maternal expression of the checkpoint protein BubR1 is required for synchrony of syncytial nuclear divisions and polar body arrest in Drosophila melanogaster. Development 132: 4509-4520 [Abstract] [Full Text]
Zhang, X.-H., Axton, J. M., Drinjakovic, J., Lorenz, L., White-Cooper, H., Renault, A. D. (2004). Spatial and temporal control of mitotic cyclins by the Gnu regulator of embryonic mitosis in Drosophila. J. Cell Sci. 117: 3571-3578 [Abstract] [Full Text]
Delattre, M., Gonczy, P. (2004). The arithmetic of centrosome biogenesis. J. Cell Sci. 117: 1619-1630 [Abstract] [Full Text]
Hinchcliffe, E. H., Sluder, G. (2001). "It Takes Two to Tango": understanding how centrosome duplication is regulated throughout the cell cycle. Genes Dev. 15: 1167-1181 [Full Text]
Foe, V., Field, C., Odell, G. (2000). Microtubules and mitotic cycle phase modulate spatiotemporal distributions of F-actin and myosin II in Drosophila syncytial blastoderm embryos. Development 127: 1767-1787 [Abstract]
Corellou, F, Bisgrove, S., Kropf, D., Meijer, L, Kloareg, B, Bouget, F. (2000). A S/M DNA replication checkpoint prevents nuclear and cytoplasmic events of cell division including centrosomal axis alignment and inhibits activation of cyclin-dependent kinase-like proteins in fucoid zygotes. Development 127: 1651-1660 [Abstract]
Santocanale, C., Sharma, K., Diffley, J. F.X. (1999). Activation of dormant origins of DNA replication in budding yeast. Genes Dev. 13: 2360-2364 [Abstract] [Full Text]
Kasravi, A., Walter, M. F., Brand, S., Mason, J. M., Biessmann, H. (1999). Molecular Cloning and Tissue-Specific Expression of the mutator2 Gene (mu2) in Drosophila melanogaster. Genetics 152: 1025-1035 [Abstract] [Full Text]
Hinchcliffe, E. H., Li, C., Thompson, E. A., Maller, J. L., Sluder, G. (1999). Requirement of Cdk2-Cyclin E Activity for Repeated Centrosome Reproduction in Xenopus Egg Extracts. Science 283: 851-854 [Abstract] [Full Text]
Ploubidou, A, Robinson, D., Docherty, R., Ogbadoyi, E., Gull, K (1999). Evidence for novel cell cycle checkpoints in trypanosomes: kinetoplast segregation and cytokinesis in the absence of mitosis. J. Cell Sci. 112: 4641-4650 [Abstract]
Hinchcliffe, E. H., Cassels, G. O., Rieder, C. L., Sluder, G. (1998). The Coordination of Centrosome Reproduction with Nuclear Events of the Cell Cycle in the Sea Urchin Zygote. JCB 140: 1417-1426 [Abstract] [Full Text]
Gonzalez, C, Tavosanis, G, Mollinari, C (1998). Centrosomes and microtubule organisation during Drosophila development. J. Cell Sci. 111: 2697-2706 [Abstract]
Reinsch, S, Gonczy, P (1998). Mechanisms of nuclear positioning. J. Cell Sci. 111: 2283-2295 [Abstract]
Jerka-Dziadosz, M, Ruiz, F, Beisson, F (1998). Uncoupling of basal body duplication and cell division in crochu, a mutant of Paramecium hypersensitive to nocodazole. Development 125: 1305-1314 [Abstract]
Hinchcliffe, E., Linck, R. (1998). Two proteins isolated from sea urchin sperm flagella: structural components common to the stable microtubules of axonemes and centrioles. J. Cell Sci. 111: 585-595 [Abstract]
Callaini, G, Dallai, R, Riparbelli, M. (1997). Wolbachia-induced delay of paternal chromatin condensation does not prevent maternal chromosomes from entering anaphase in incompatible crosses of Drosophila simulans. J. Cell Sci. 110: 271-280 [Abstract]
Edgar, B A, Datar, S A (1996). Zygotic degradation of two maternal Cdc25 mRNAs terminates Drosophila's early cell cycle program.. Genes Dev. 10: 1966-1977 [Abstract]
Frenz, L., Glover, D. (1996). A maternal requirement for glutamine synthetase I for the mitotic cycles of syncytial Drosophila embryos. J. Cell Sci. 109: 2649-2660 [Abstract]
Woods, C., Zhu, J, Coleman, T, Bloom, S., Lazarides, E (1995). Novel centrosomal protein reveals the presence of multiple centrosomes in turkey (Meleagris gallopavo) bnbn binucleated erythrocytes. J. Cell Sci. 108: 699-710 [Abstract]
Fogarty, P, Kalpin, R., Sullivan, W (1994). The Drosophila maternal-effect mutation grapes causes a metaphase arrest at nuclear cycle 13. Development 120: 2131-2142 [Abstract]
Williams, B., Goldberg, M. (1994). Determinants of Drosophila zw10 protein localization and function. J. Cell Sci. 107: 785-798 [Abstract]
Endow, S., Chandra, R, Komma, D., Yamamoto, A., Salmon, E. (1994). Mutants of the Drosophila ncd microtubule motor protein cause centrosomal and spindle pole defects in mitosis. J. Cell Sci. 107: 859-867 [Abstract]
White-Cooper, H, Alphey, L, Glover, D. (1993). The cdc25 homologue twine is required for only some aspects of the entry into meiosis in Drosophila. J. Cell Sci. 106: 1035-1044 [Abstract]
Liu, Q, Golubovskaya, I, Cande, W. (1993). Abnormal cytoskeletal and chromosome distribution in po, ms4 and ms6; mutant alleles of polymitotic that disrupt the cell cycle progression from meiosis to mitosis in maize. J. Cell Sci. 106: 1169-1178 [Abstract]
Hegde, J, Stephenson, E. (1993). Distribution of swallow protein in egg chambers and embryos of Drosophila melanogaster. Development 119: 457-470 [Abstract]
Philp, A., Axton, J., Saunders, R., Glover, D. (1993). Mutations in the Drosophila melanogaster gene three rows permit aspects of mitosis to continue in the absence of chromatid segregation. J. Cell Sci. 106: 87-98 [Abstract]
Buchenau, P, Saumweber, H, Arndt-Jovin, D. (1993). Consequences of topoisomerase II inhibition in early embryogenesis of Drosophila revealed by in vivo confocal laser scanning microscopy. J. Cell Sci. 104: 1175-1185 [Abstract]
Gomes, R, Karess, R., Ohkura, H, Glover, D., Sunkel, C. (1993). Abnormal anaphase resolution (aar): a locus required for progression through mitosis in Drosophila. J. Cell Sci. 104: 583-593 [Abstract]
Llamazares, S, Moreira, A, Tavares, A, Girdham, C, Spruce, B A, Gonzalez, C, Karess, R E, Glover, D M, Sunkel, C E (1991). polo encodes a protein kinase homolog required for mitosis in Drosophila.. Genes Dev. 5: 2153-2165 [Abstract]
Girdham, C H, Glover, D M (1991). Chromosome tangling and breakage at anaphase result from mutations in lodestar, a Drosophila gene encoding a putative nucleoside triphosphate-binding protein.. Genes Dev. 5: 1786-1799 [Abstract]
Murray, A.W. (1991). Coordinating Cell Cycle Events. Cold Spring Harb Symp Quant Biol 56: 399-408 [Abstract]
Kellogg, D.R., Sullivan, W., Theurkauf, W., Oegema, K., Raff, J.W., Alberts, B.M. (1991). Studies on the Centrosome and Cytoplasmic Organization in the Early Drosophila Embryo. Cold Spring Harb Symp Quant Biol 56: 649-662 [Abstract]
Glover, D.M., Llamazares, S., Girdham, C., Maldonado-Codina, G., Moreira, A., Tavares, A., Sunkel, C.E., Gonzalez, C. (1991). Cyclical Changes in the Subcellular Distribution of Proteins Essential for Mitosis during Embryogenesis in Drosophila. Cold Spring Harb Symp Quant Biol 56: 709-717 [Abstract]
Lin, H., Song, K., Hutcheson, G.A., Goutte, C.E., Wolfner, M.F. (1991). A Maternally Encoded Nuclear Envelope Protein Required for Embryonic Mitosis in Drosophila. Cold Spring Harb Symp Quant Biol 56: 719-727 [Abstract]
Hartwell, L., Weinert, T. (1989). Checkpoints: controls that ensure the order of cell cycle events. Science 246: 629-634 [Abstract]