Sequential activation of alpha-actin genes during avian cardiogenesis: vascular smooth muscle alpha-actin gene transcripts mark the onset of cardiomyocyte differentiation

doi:10.1083/jcb.107.6.2575

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Sequential activation of alpha-actin genes during avian cardiogenesis: vascular smooth muscle alpha-actin gene transcripts mark the onset of cardiomyocyte differentiation

DL Ruzicka and RJ Schwartz
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.

The expression of cytoplasmic beta-actin and cardiac, skeletal, and smooth muscle alpha-actins during early avian cardiogenesis was analyzed by in situ hybridization with mRNA-specific single-stranded DNA probes. The cytoplasmic beta-actin gene was ubiquitously expressed in the early chicken embryo. In contrast, the alpha-actin genes were sequentially activated in avian cardiac tissue during the early stages of heart tube formation. The accumulation of large quantities of smooth muscle alpha-actin transcripts in epimyocardial cells preceded the expression of the sarcomeric alpha-actin genes. The accumulation of skeletal alpha-actin mRNAs in the developing heart lagged behind that of cardiac alpha-actin by several embryonic stages. At Hamburger- Hamilton stage 12, the smooth muscle alpha-actin gene was selectively down-regulated in the heart such that only the conus, which subsequently participates in the formation of the vascular trunks, continued to express this gene. This modulation in smooth muscle alpha- actin gene expression correlated with the beginning of coexpression of sarcomeric alpha-actin transcripts in the epimyocardium and the onset of circulation in the embryo. The specific expression of the vascular smooth muscle alpha-actin gene marks the onset of differentiation of cardiac cells and represents the first demonstration of coexpression of both smooth muscle and striated alpha-actin genes within myogenic cells.
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Hungerford, J. E., Hoeffler, J. P., Bowers, C. W., Dahm, L. M., Falchetto, R., Shabanowitz, J., Hunt, D. F., Little, C. D. (1997). Identification of a Novel Marker for Primordial Smooth Muscle and Its Differential Expression Pattern in Contractile vs Noncontractile Cells. JCB 137: 925-937 [Abstract] [Full Text]
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Miano, J. M., Olson, E. N. (1996). Expression of the Smooth Muscle Cell Calponin Gene Marks the Early Cardiac and Smooth Muscle Cell Lineages during Mouse Embryogenesis. J. Biol. Chem. 271: 7095-7103 [Abstract] [Full Text]
Li, L., Miano, J. M., Cserjesi, P., Olson, E. N. (1996). SM22{alpha}, a Marker of Adult Smooth Muscle, Is Expressed in Multiple Myogenic Lineages During Embryogenesis. Circ. Res. 78: 188-195 [Abstract] [Full Text]
Samaha, F. F., Ip, H. S., Morrisey, E. E., Seltzer, J., Tang, Z., Solway, J., Parmacek, M. S. (1996). Developmental Pattern of Expression and Genomic Organization of the Calponin-h1 Gene. J. Biol. Chem. 271: 395-403 [Abstract] [Full Text]
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Saint-Jeannet, J., Levi, G, Girault, J., Koteliansky, V, Thiery, J. (1992). Ventrolateral regionalization of Xenopus laevis mesoderm is characterized by the expression of alpha-smooth muscle actin. Development 115: 1165-1173 [Abstract]
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