Amino acid sequence and domain structure of entactin. Homology with epidermal growth factor precursor and low density lipoprotein receptor

doi:10.1083/jcb.107.6.2749

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Amino acid sequence and domain structure of entactin. Homology with epidermal growth factor precursor and low density lipoprotein receptor

ME Durkin, S Chakravarti, BB Bartos, SH Liu, RL Friedman and AE Chung
Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260.

Entactin (nidogen), a 150-kD sulfated glycoprotein, is a major component of basement membranes and forms a highly stable noncovalent complex with laminin. The complete amino acid sequence of mouse entactin has been derived from sequencing of cDNA clones. The 5.9-kb cDNA contains a 3,735-bp open reading frame followed by a 3'- untranslated region of 2.2 kb. The open reading frame encodes a 1,245- residue polypeptide with an unglycosylated Mr of 136,500, a 28-residue signal peptide, two Asn-linked glycosylation sites, and two potential Ca2+-binding sites. Analysis of the deduced amino acid sequence predicts that the molecule consists of two globular domains of 70 and 36 kD separated by a cysteine-rich domain of 28 kD. The COOH-terminal globular domain shows homology to the EGF precursor and the low density lipoprotein receptor. Entactin contains six EGF-type cysteine-rich repeat units and one copy of a cysteine-repeat motif found in thyroglobulin. The Arg-Gly-Asp cell recognition sequence is present in one of the EGF-type repeats, and a synthetic peptide from the putative cell-binding site of entactin was found to promote the attachment of mouse mammary tumor cells.
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Balzar, M., Briaire-de Bruijn, I. H., Rees-Bakker, H. A. M., Prins, F. A., Helfrich, W., de Leij, L., Riethmüller, G., Alberti, S., Warnaar, S. O., Fleuren, G. J., Litvinov, S. V. (2001). Epidermal Growth Factor-Like Repeats Mediate Lateral and Reciprocal Interactions of Ep-CAM Molecules in Homophilic Adhesions. Mol. Cell. Biol. 21: 2570-2580 [Abstract] [Full Text]
Kang, S. H., Kramer, J. M. (2000). Nidogen Is Nonessential and Not Required for Normal Type IV Collagen Localization in Caenorhabditis elegans. Mol. Biol. Cell 11: 3911-3923 [Abstract] [Full Text]
Murshed, M., Smyth, N., Miosge, N., Karolat, J., Krieg, T., Paulsson, M., Nischt, R. (2000). The Absence of Nidogen 1 Does Not Affect Murine Basement Membrane Formation. Mol. Cell. Biol. 20: 7007-7012 [Abstract] [Full Text]
Pujuguet, P, Simian, M, Liaw, J, Timpl, R, Werb, Z, Bissell, M. (2000). Nidogen-1 regulates laminin-1-dependent mammary-specific gene expression. J. Cell Sci. 113: 849-858 [Abstract]
Legan, P. K., Rau, A., Keen, J. N., Richardson, G. P. (1997). The Mouse Tectorins. MODULAR MATRIX PROTEINS OF THE INNER EAR HOMOLOGOUS TO COMPONENTS OF THE SPERM-EGG ADHESION SYSTEM. J. Biol. Chem. 272: 8791-8801 [Abstract] [Full Text]
Reinhardt, D. P., Ono, R. N., Sakai, L. Y. (1997). Calcium Stabilizes Fibrillin-1 against Proteolytic Degradation. J. Biol. Chem. 272: 1231-1236 [Abstract] [Full Text]
Kadoya, Y, Salmivirta, K, Talts, J., Kadoya, K, Mayer, U, Timpl, R, Ekblom, P (1997). Importance of nidogen binding to laminin gamma1 for branching epithelial morphogenesis of the submandibular gland. Development 124: 683-691 [Abstract]
Gresham, H. D., Graham, I. L., Griffin, G. L., Hsieh, J.-C., Dong, L.-J., Chung, AlbertE., Senior, RobertM. (1996). Domain-specific Interactions between Entactin and Neutrophil Integrins. G2 DOMAIN LIGATION OF INTEGRIN alpha 3beta 1 AND E DOMAIN LIGATION OF THE LEUKOCYTE RESPONSE INTEGRIN SIGNAL FOR DIFFERENT RESPONSES. J. Biol. Chem. 271: 30587-30594 [Abstract] [Full Text]
Yamazaki, H., Bujo, H., Kusunoki, J., Seimiya, K., Kanaki, T., Morisaki, N., Schneider, W. J., Saito, Y. (1996). Elements of Neural Adhesion Molecules and a Yeast Vacuolar Protein Sorting Receptor Are Present in a Novel Mammalian Low Density Lipoprotein Receptor Family Member. J. Biol. Chem. 271: 24761-24768 [Abstract] [Full Text]
Yamashita, M., Konagaya, S. (1996). A Novel Cysteine Protease Inhibitor of the Egg of Chum Salmon, Containing a Cysteine-rich Thyroglobulin-like Motif. J. Biol. Chem. 271: 1282-1284 [Abstract] [Full Text]
Dong, L.-J., Hsieh, J.-C., Chung, A. E. (1995). Two Distinct Cell Attachment Sites in Entactin Are Revealed by Amino Acid Substitutions and Deletion of the RGD Sequence in the Cysteine-rich Epidermal Growth Factor Repeat 2. J. Biol. Chem. 270: 15838-15843 [Abstract] [Full Text]
Wu, C, Chung, A., McDonald, J. (1995). A novel role for alpha 3 beta 1 integrins in extracellular matrix assembly. J. Cell Sci. 108: 2511-2523 [Abstract]
Ekblom, P, Ekblom, M, Fecker, L, Klein, G, Zhang, H., Kadoya, Y, Chu, M., Mayer, U, Timpl, R (1994). Role of mesenchymal nidogen for epithelial morphogenesis in vitro. Development 120: 2003-2014 [Abstract]
Shum, L, Sakakura, Y, Bringas, P, Luo, W, Snead, M., Mayo, M, Crohin, C, Millar, S, Werb, Z, Buckley, S, et, al. (1993). EGF abrogation-induced fusilli-form dysmorphogenesis of Meckel's cartilage during embryonic mouse mandibular morphogenesis in vitro. Development 118: 903-917 [Abstract]
Milos, N. C. (1992). Mesoderm and Jaw Development in Vertebrates: The Role of Growth Factors. CROBM 4: 73-91 [Abstract] [Full Text]