The Journal of Cell Biology, Vol 108, 653-660, Copyright © 1989 by The Rockefeller University Press

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Transforming growth factor-beta 1: histochemical localization with antibodies to different epitopes

KC Flanders, NL Thompson, DS Cissel, E Van Obberghen-Schilling, CC Baker, ME Kass, LR Ellingsworth, AB Roberts and MB Sporn
Laboratory of Chemoprevention, National Cancer Institute, Bethesda, Maryland 20892.

We have localized transforming growth factor-beta (TGF-beta) in many cells and tissues with immunohistochemical methods, using two polyclonal antisera raised to different synthetic preparations of a peptide corresponding to the amino-terminal 30 amino acids of TGF-beta 1. These two antibodies give distinct staining patterns; the staining by anti-CC(1-30) is intracellular. This differential staining pattern is consistently observed in several systems, including cultured tumor cells; mouse embryonic, neonatal, and adult tissues; bovine fibropapillomas; and human colon carcinomas. The extracellular staining by anti-CC(1-30) partially resembles that seen with an antibody to fibronectin, suggesting that extracellular TGF-beta may be bound to matrix proteins. The intracellular staining by anti-LC(1-30) is similar to that seen with two other antibodies raised to peptides corresponding to either amino acids 266-278 of the TGF-beta 1 precursor sequence or to amino acids 50-75 of mature TGF-beta 1, suggesting that anti-LC(1- 30) stains sites of TGF-beta synthesis. Results from RIA and ELISAs indicate that anti-LC(1-30) and anti-CC(1-30) recognize different epitopes of this peptide and of TGF-beta 1 itself.
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• Letterio, J. J., Lehrnbecher, T., Pollack, G., Walsh, T. J., Chanock, S. J. (2001). Invasive Candidiasis Stimulates Hepatocyte and Monocyte Production of Active Transforming Growth Factor {beta}. Infect. Immun. 69: 5115-5120 [Abstract] [Full Text]  
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• Dunker, N., Schuster, N., Krieglstein, K. (2001). TGF-{beta} modulates programmed cell death in the retina of the developing chick embryo. Development 128: 1933-1942 [Abstract] [Full Text]  
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• Roy, S. K., Kurz, S. G., Carlson, A. M., DeJonge, C. J., Ramey, J. W., Maclin, V. M. (1998). Transforming Growth Factor ß Receptor Expression in Hyperstimulated Human Granulosa Cells and Cleavage Potential of the Zygotes. Biol. Reprod. 59: 1311-1316 [Abstract] [Full Text]  
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• Wang, J., Zheng, H., Sung, C.-C., Richter, K. K., Hauer-Jensen, M. (1998). Cellular Sources of Transforming Growth Factor-ß Isoforms in Early and Chronic Radiation Enteropathy. Am. J. Pathol. 153: 1531-1540 [Abstract] [Full Text]  
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• Ye, L., Mora, R., Akhayani, N., Haudenschild, C. C., Liau, G. (1997). Growth Factor and Cytokine-Regulated Hyaluronan-Binding Protein TSG-6 Is Localized to the Injury-Induced Rat Neointima and Confers Enhanced Growth in Vascular Smooth Muscle Cells. Circ. Res. 81: 289-296 [Abstract] [Full Text]  
• Shi, Y., O'Brien, J. E., Fard, A., Zalewski, A. (1996). Transforming Growth Factor-ß1 Expression and Myofibroblast Formation During Arterial Repair. Arterioscler. Thromb. Vasc. Bio. 16: 1298-1305 [Abstract] [Full Text]  
• Hoffman, M., Kibbey, M., Letterio, J., Kleinman, H. (1996). Role of laminin-1 and TGF-beta 3 in acinar differentiation of a human submandibular gland cell line (HSG). J. Cell Sci. 109: 2013-2021 [Abstract]  
• Cui, W, Fowlis, D J, Cousins, F M, Duffie, E, Bryson, S, Balmain, A, Akhurst, R J (1995). Concerted action of TGF-beta 1 and its type II receptor in control of epidermal homeostasis in transgenic mice.. Genes Dev. 9: 945-955 [Abstract]  
• Taipale, J., Lohi, J., Saarinen, J., Kovanen, P. T., Keski-Oja, J. (1995). Human Mast Cell Chymase and Leukocyte Elastase Release Latent Transforming Growth Factor-beta1 from the Extracellular Matrix of Cultured Human Epithelial and Endothelial Cells. J. Biol. Chem. 270: 4689-4696 [Abstract] [Full Text]  
• Clark, R., Nielsen, L., Welch, M., McPherson, J. (1995). Collagen matrices attenuate the collagen-synthetic response of cultured fibroblasts to TGF-beta. J. Cell Sci. 108: 1251-1261 [Abstract]  
• Glick, A B, Lee, M M, Darwiche, N, Kulkarni, A B, Karlsson, S, Yuspa, S H (1994). Targeted deletion of the TGF-beta 1 gene causes rapid progression to squamous cell carcinoma.. Genes Dev. 8: 2429-2440 [Abstract]  
• Letterio, J., Geiser, A., Kulkarni, A., Roche, N., Sporn, M., Roberts, A. (1994). Maternal rescue of transforming growth factor-beta 1 null mice. Science 264: 1936-1938 [Abstract]  
• Critchlow, M., Bland, Y., Ashhurst, D. (1994). The effects of age on the response of rabbit periosteal osteoprogenitor cells to exogenous transforming growth factor-beta 2. J. Cell Sci. 107: 499-516 [Abstract]  
• Dickson, M., Slager, H., Duffie, E, Mummery, C., Akhurst, R. (1993). RNA and protein localisations of TGF beta 2 in the early mouse embryo suggest an involvement in cardiac development. Development 117: 625-639 [Abstract]  
• Sieweke, M., Thompson, N., Sporn, M., Bissell, M. (1990). Mediation of wound-related Rous sarcoma virus tumorigenesis by TGF-beta. Science 248: 1656-1660 [Abstract]  
• Lyons, K M, Pelton, R W, Hogan, B L (1989). Patterns of expression of murine Vgr-1 and BMP-2a RNA suggest that transforming growth factor-beta-like genes coordinately regulate aspects of embryonic development.. Genes Dev. 3: 1657-1668 [Abstract]  

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