Higher order chromosome structure is affected by cold-sensitive mutations in a Schizosaccharomyces pombe gene crm1+ which encodes a 115- kD protein preferentially localized in the nucleus and its periphery

doi:10.1083/jcb.108.4.1195

This Article

	Full Text (PDF, 3620K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Adachi, Y.
	Articles by Yanagida, M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Adachi, Y.
	Articles by Yanagida, M.


Social Bookmarking

	What's this?

ARTICLES

Higher order chromosome structure is affected by cold-sensitive mutations in a Schizosaccharomyces pombe gene crm1+ which encodes a 115- kD protein preferentially localized in the nucleus and its periphery

Y Adachi and M Yanagida
Department of Biophysics, Faculty of Science, Kyoto University, Japan.

We isolated a novel class of Schizosaccharomyces pombe cold-sensitive mutants with deformed nuclear chromosome domains consisting of thread- or rodlike condensed segments at restrictive temperature. Their mutations were mapped in a novel, identical locus designated crm1 (chromosomal region maintenance). The crm1 mutants also show the following phenotypes. DNA, RNA, and protein syntheses diminish at restrictive temperature. At permissive temperature, the amount of one particular protein, p25, greatly increases. The mutant growth is hypersensitive to Ca2+ and resistant to protein kinase inhibitors. We cloned the 4.1-kb-long crm1+ gene that rescued the above phenotypes by transformation and determined its nucleotide sequence, which predicts a 1,077-residue protein. Affinity-purified antiserum raised against the crm1+ polypeptide expressed in Escherichia coli detected a 115-kD protein in S. pombe extracts. Genomic Southern hybridization and immunoblotting suggested that the crm1+ product might be highly conserved in distant organisms. Through immunofluorescence microscopy, the crm1+ protein appeared to be principally localized within the nucleus and also at its periphery. We speculate that the crm1+ protein might be one of those nuclear components that modify the chromosome structures or regulate the nuclear environment required for maintaining higher order chromosome structures.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Hanyu, Y., Imai, K. K., Kawasaki, Y., Nakamura, T., Nakaseko, Y., Nagao, K., Kokubu, A., Ebe, M., Fujisawa, A., Hayashi, T., Obuse, C., Yanagida, M. (2009). Schizosaccharomyces pombe cell division cycle under limited glucose requires Ssp1 kinase, the putative CaMKK, and Sds23, a PP2A-related phosphatase inhibitor. GENES CELLS 14: 539-554 [Abstract] [Full Text]
Blanvillain, R., Boavida, L. C., McCormick, S., Ow, D. W. (2008). EXPORTIN1 Genes Are Essential for Development and Function of the Gametophytes in Arabidopsis thaliana. Genetics 180: 1493-1500 [Abstract] [Full Text]
Hayashi, T., Hatanaka, M., Nagao, K., Nakaseko, Y., Kanoh, J., Kokubu, A., Ebe, M., Yanagida, M. (2007). Rapamycin sensitivity of the Schizosaccharomyces pombe tor2 mutant and organization of two highly phosphorylated TOR complexes by specific and common subunits.. GENES CELLS 12: 1357-1370 [Abstract] [Full Text]
Takayanagi, R., Ohashi, T., Yamashita, E., Kurosaki, Y., Tanaka, K., Hakata, Y., Komoda, Y., Ikeda, S., Tsunetsugu-Yokota, Y., Tanaka, Y., Shida, H. (2007). Enhanced Replication of Human T-Cell Leukemia Virus Type 1 in T Cells from Transgenic Rats Expressing Human CRM1 That Is Regulated in a Natural Manner. J. Virol. 81: 5908-5918 [Abstract] [Full Text]
Bernreiter, A., Ramon, A., Fernandez-Martinez, J., Berger, H., Araujo-Bazan, L., Espeso, E. A., Pachlinger, R., Gallmetzer, A., Anderl, I., Scazzocchio, C., Strauss, J. (2007). Nuclear Export of the Transcription Factor NirA Is a Regulatory Checkpoint for Nitrate Induction in Aspergillus nidulans. Mol. Cell. Biol. 27: 791-802 [Abstract] [Full Text]
Nagao, K., Yanagida, M. (2006). Securin can have a separase cleavage site by substitution mutations in the domain required for stabilization and inhibition of separase. GENES CELLS 11: 247-260 [Abstract] [Full Text]
Daelemans, D., Costes, S. V., Lockett, S., Pavlakis, G. N. (2005). Kinetic and Molecular Analysis of Nuclear Export Factor CRM1 Association with Its Cargo In Vivo. Mol. Cell. Biol. 25: 728-739 [Abstract] [Full Text]
Lopez-Bueno, A., Valle, N., Gallego, J. M., Perez, J., Almendral, J. M. (2004). Enhanced Cytoplasmic Sequestration of the Nuclear Export Receptor CRM1 by NS2 Mutations Developed in the Host Regulates Parvovirus Fitness. J. Virol. 78: 10674-10684 [Abstract] [Full Text]
Kimata, Y., Yanagida, M. (2004). Suppression of a mitotic mutant by tRNA-Ala anticodon mutations that produce a dominant defect in late mitosis. J. Cell Sci. 117: 2283-2293 [Abstract] [Full Text]
Bernad, R., van der Velde, H., Fornerod, M., Pickersgill, H. (2004). Nup358/RanBP2 Attaches to the Nuclear Pore Complex via Association with Nup88 and Nup214/CAN and Plays a Supporting Role in CRM1-Mediated Nuclear Protein Export. Mol. Cell. Biol. 24: 2373-2384 [Abstract] [Full Text]
Murphy, M. W., Olson, B. L., Siliciano, P. G. (2004). The Yeast Splicing Factor Prp40p Contains Functional Leucine-Rich Nuclear Export Signals That Are Essential for Splicing. Genetics 166: 53-65 [Abstract] [Full Text]
Moye-Rowley, W. S. (2003). Regulation of the Transcriptional Response to Oxidative Stress in Fungi: Similarities and Differences. Eukaryot Cell 2: 381-389 [Full Text]
Lagreid, A., Hvidsten, T. R., Midelfart, H., Komorowski, J., Sandvik, A. K. (2003). Predicting Gene Ontology Biological Process From Temporal Gene Expression Patterns. Genome Res 13: 965-979 [Abstract] [Full Text]
Naresh, A., Saini, S., Singh, J. (2003). Identification of Uhp1, a Ubiquitinated Histone-like Protein, as a Target/Mediator of Rhp6 in Mating-type Silencing in Fission Yeast. J. Biol. Chem. 278: 9185-9194 [Abstract] [Full Text]
Cai, Y., Singh, B. B., Aslanukov, A., Zhao, H., Ferreira, P. A. (2001). The Docking of Kinesins, KIF5B and KIF5C, to Ran-binding Protein 2 (RanBP2) Is Mediated via a Novel RanBP2 Domain. J. Biol. Chem. 276: 41594-41602 [Abstract] [Full Text]
Kim, Y.-K., Liu, Z.-M., Li, D., Kolattukudy, P. E. (2000). Two Novel Genes Induced by Hard-Surface Contact of Colletotrichum gloeosporioides Conidia. J. Bacteriol. 182: 4688-4695 [Abstract] [Full Text]
Collier, S., Chan, H. Y. E., Toda, T., McKimmie, C., Johnson, G., Adler, P. N., O'Kane, C., Ashburner, M. (2000). The Drosophila embargoed Gene Is Required for Larval Progression and Encodes the Functional Homolog of Schizosaccharomyces Crm1. Genetics 155: 1799-1807 [Abstract] [Full Text]
Tanaka, K., Yonekawa, T., Kawasaki, Y., Kai, M., Furuya, K., Iwasaki, M., Murakami, H., Yanagida, M., Okayama, H. (2000). Fission Yeast Eso1p Is Required for Establishing Sister Chromatid Cohesion during S Phase. Mol. Cell. Biol. 20: 3459-3469 [Abstract] [Full Text]
Fasken, M. B., Saunders, R., Rosenberg, M., Brighty, D. W. (2000). A Leptomycin B-sensitive Homologue of Human CRM1 Promotes Nuclear Export of Nuclear Export Sequence-containing Proteins in Drosophila Cells. J. Biol. Chem. 275: 1878-1886 [Abstract] [Full Text]
Liu, J., DeFranco, D. B. (2000). Protracted Nuclear Export of Glucocorticoid Receptor Limits Its Turnover and Does Not Require the Exportin 1/CRM1-Directed Nuclear Export Pathway. Mol. Endocrinol. 14: 40-51 [Abstract] [Full Text]
Singh, B. B., Patel, H. H., Roepman, R., Schick, D., Ferreira, P. A. (1999). The Zinc Finger Cluster Domain of RanBP2 Is a Specific Docking Site for the Nuclear Export Factor, Exportin-1. J. Biol. Chem. 274: 37370-37378 [Abstract] [Full Text]
Bodendorf, U., Cziepluch, C., Jauniaux, J.-C., Rommelaere, J., Salomé, N. (1999). Nuclear Export Factor CRM1 Interacts with Nonstructural Proteins NS2 from Parvovirus Minute Virus of Mice. J. Virol. 73: 7769-7779 [Abstract] [Full Text]
Kudo, N., Matsumori, N., Taoka, H., Fujiwara, D., Schreiner, E. P., Wolff, B., Yoshida, M., Horinouchi, S. (1999). Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue in the central conserved region. Proc. Natl. Acad. Sci. USA 96: 9112-9117 [Abstract] [Full Text]
Shibuya, T., Tsuneyoshi, S., Azad, A. K., Urushiyama, S., Ohshima, Y., Tani, T. (1999). Characterization of the ptr6+ Gene in Fission Yeast: A Possible Involvement of a Transcriptional Coactivator TAF in Nucleocytoplasmic Transport of mRNA. Genetics 152: 869-880 [Abstract] [Full Text]
Goshima, G., Saitoh, S., Yanagida, M. (1999). Proper metaphase spindle length is determined by centromere proteins Mis12 and Mis6 required for faithful chromosome segregation. Genes Dev. 13: 1664-1677 [Abstract] [Full Text]
Farjot, G., Sergeant, A., Mikaelian, I. (1999). A New Nucleoporin-like Protein Interacts with Both HIV-1 Rev Nuclear Export Signal and CRM-1. J. Biol. Chem. 274: 17309-17317 [Abstract] [Full Text]
Floer, M., Blobel, G. (1999). Putative Reaction Intermediates in Crm1-mediated Nuclear Protein Export. J. Biol. Chem. 274: 16279-16286 [Abstract] [Full Text]
Kudo, N., Taoka, H., Toda, T., Yoshida, M., Horinouchi, S. (1999). A Novel Nuclear Export Signal Sensitive to Oxidative Stress in the Fission Yeast Transcription Factor Pap1. J. Biol. Chem. 274: 15151-15158 [Abstract] [Full Text]
Holaska, J. M., Paschal, B. M. (1998). A cytosolic activity distinct from Crm1 mediates nuclear export of protein kinase inhibitor in permeabilized cells. Proc. Natl. Acad. Sci. USA 95: 14739-14744 [Abstract] [Full Text]
Toone, W. M., Kuge, S., Samuels, M., Morgan, B. A., Toda, T., Jones, N. (1998). Regulation of the fission yeast transcription factor Pap1 by oxidative stress: requirement for the nuclear export factor Crm1 (Exportin) and the stress-activated MAP kinase�Sty1/Spc1. Genes Dev. 12: 1453-1463 [Abstract] [Full Text]
Jin, Q.-w., Trelles-Sticken, E., Scherthan, H., Loidl, J. (1998). Yeast Nuclei Display Prominent Centromere Clustering That Is Reduced in Nondividing Cells and in Meiotic Prophase. JCB 141: 21-29 [Abstract] [Full Text]
Shen, E. C., Henry, M. F., Weiss, V. H., Valentini, S. R., Silver, P. A., Lee, M. S. (1998). Arginine methylation facilitates the nuclear export of hnRNP�proteins. Genes Dev. 12: 679-691 [Abstract] [Full Text]
(1998). Isolation of a Schizosaccharomyces pombe rad21ts Mutant That Is Aberrant in Chromosome Segregation, Microtubule Function, DNA Repair and Sensitive to Hydroxyurea: Possible Involvement of Rad21 in Ubiquitin-Mediated Proteolysis. Genetics 148: 49-58
Kudo, N., Khochbin, S., Nishi, K., Kitano, K., Yanagida, M., Yoshida, M., Horinouchi, S. (1997). Molecular Cloning and Cell Cycle-dependent Expression of Mammalian CRM1, a Protein Involved in Nuclear Export of Proteins. J. Biol. Chem. 272: 29742-29751 [Abstract] [Full Text]
Ossareh-Nazari, B., Bachelerie, F., Dargemont, C. (1997). Evidence for a Role of CRM1 in Signal-Mediated Nuclear Protein Export. Science 278: 141-144 [Abstract] [Full Text]
Collier, S, Gubb, D (1997). Drosophila tissue polarity requires the cell-autonomous activity of the fuzzy gene, which encodes a novel transmembrane protein. Development 124: 4029-4037 [Abstract]
Shimanuki, M, Saka, Y, Yanagida, M, Toda, T (1995). A novel essential fission yeast gene pad1+ positively regulates pap1(+)-dependent transcription and is implicated in the maintenance of chromosome structure. J. Cell Sci. 108: 569-579 [Abstract]
Kinoshita, N, Yamano, H, Niwa, H, Yoshida, T, Yanagida, M (1993). Negative regulation of mitosis by the fission yeast protein phosphatase ppa2.. Genes Dev. 7: 1059-1071 [Abstract]
Samejima, I, Matsumoto, T, Nakaseko, Y, Beach, D, Yanagida, M (1993). Identification of seven new cut genes involved in Schizosaccharomyces pombe mitosis. J. Cell Sci. 105: 135-143 [Abstract]
Sueishi, M., Takagi, M., Yoneda, Y. (2000). The Forkhead-associated Domain of Ki-67 Antigen Interacts with the Novel Kinesin-like Protein Hklp2. J. Biol. Chem. 275: 28888-28892 [Abstract] [Full Text]
Forgues, M., Marrogi, A. J., Spillare, E. A., Wu, C.-G., Yang, Q., Yoshida, M., Wang, X. W. (2001). Interaction of the Hepatitis B Virus X Protein with the Crm1-dependent Nuclear Export Pathway. J. Biol. Chem. 276: 22797-22803 [Abstract] [Full Text]
Takagi, M., Sueishi, M., Saiwaki, T., Kametaka, A., Yoneda, Y. (2001). A Novel Nucleolar Protein, NIFK, Interacts with the Forkhead Associated Domain of Ki-67 Antigen in Mitosis. J. Biol. Chem. 276: 25386-25391 [Abstract] [Full Text]