The SPA2 protein of yeast localizes to sites of cell growth

doi:10.1083/jcb.108.4.1419

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The SPA2 protein of yeast localizes to sites of cell growth

M Snyder
Department of Biology, Yale University, New Haven, Connecticut 06511.

A yeast gene, SPA2, was isolated with human anti-spindle pole autoantibodies. The SPA2 gene was fused to the Escherichia coli trpE gene, and polyclonal antibodies were prepared to the fusion protein. Immunofluorescence experiments indicate that the SPA2 gene product has a sharply polarized distribution in yeast cells. In budded cells the SPA2 protein is present at the tip of the bud; in unbudded cells, it is localized to one edge of the cell. When a-cells are induced to form schmoos with alpha-factor, the SPA2 protein is found at the tip of the schmoo. These areas of SPA2 localization correspond to cellular sites expected to be involved in bud formation and/or cell growth. The SPA2 antigen is present in a-cells, alpha-cells, and a/alpha-diploid cells, but is absent in mutant cells in which the SPA2 gene has been disrupted. spa2 mutant cells are viable, but display defects in the direction and control of cell growth. Compared to wild-type cells, spa2 mutant cells have slightly altered budding patterns. Entry into stationary phase is impaired for spa2 mutants, and mutants with one particular allele, spa2-7, form multiple buds under nutrient-limiting conditions. Thus, SPA2 is a newly identified yeast gene that is involved in the direction and control of cell division, and whose gene product localizes to the site of cell growth.
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Pruyne, D, Bretscher, A (2000). Polarization of cell growth in yeast. I. Establishment and maintenance of polarity states. J. Cell Sci. 113: 365-375 [Abstract]
Pruyne, D, Bretscher, A (2000). Polarization of cell growth in yeast. J. Cell Sci. 113: 571-585 [Abstract]
Park, H.-O., Sanson, A., Herskowitz, I. (1999). Localization of Bud2p, a GTPase-activating protein necessary for programming cell polarity in yeast to the presumptive bud site. Genes Dev. 13: 1912-1917 [Abstract] [Full Text]
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Kim, J., Hirsch, J. P. (1998). A Nucleolar Protein That Affects Mating Efficiency in Saccharomyces cerevisiae by Altering the Morphological Response to Pheromone. Genetics 149: 795-805 [Abstract] [Full Text]
Gammie, A. E., Brizzio, V., Rose, M. D. (1998). Distinct Morphological Phenotypes of Cell Fusion Mutants. Mol. Biol. Cell 9: 1395-1410 [Abstract] [Full Text]
Fujiwara, T., Tanaka, K., Mino, A., Kikyo, M., Takahashi, K., Shimizu, K., Takai, Y. (1998). Rho1p-Bni1p-Spa2p Interactions: Implication in Localization of Bni1p at the Bud Site and Regulation of the Actin Cytoskeleton in Saccharomyces cerevisiae. Mol. Biol. Cell 9: 1221-1233 [Abstract] [Full Text]
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Arkowitz, R. A., Lowe, N. (1997). A Small Conserved Domain in the Yeast Spa2p Is Necessary and Sufficient for Its Polarized Localization. JCB 138: 17-36 [Abstract] [Full Text]
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Herskowitz, I., Park, H.-O., Sanders, S., Valtz, N., Peter, M. (1995). Programming of Cell Polarity in Budding Yeast by Endogenous and Exogenous Signals. Cold Spring Harb Symp Quant Biol 60: 717-727 [Abstract]
Pringle, J.R., Bi, E., Harkins, H.A., Zahner, J.E., De Virgilio, C., Chant, J., Corrado, K., Fares, H. (1995). Establishment of Cell Polarity in Yeast. Cold Spring Harb Symp Quant Biol 60: 729-744 [Abstract]
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Nelson, W. (1992). Regulation of cell surface polarity from bacteria to mammals. Science 258: 948-955 [Abstract]
Page, B D, Snyder, M (1992). CIK1: a developmentally regulated spindle pole body-associated protein important for microtubule functions in Saccharomyces cerevisiae.. Genes Dev. 6: 1414-1429 [Abstract]
Shannon, K W, Guthrie, C (1991). Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs.. Genes Dev. 5: 773-785 [Abstract]