Antibodies specific to acetylated histones document the existence of deposition- and transcription-related histone acetylation in Tetrahymena

doi:10.1083/jcb.108.5.1577

This Article

Full Text (PDF, 7330K)

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JCB

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Lin, R.

Articles by Allis, C. D.

Search for Related Content

PubMed

PubMed Citation

Articles by Lin, R.

Articles by Allis, C. D.

Pubmed/NCBI databases

Substance via MeSH

Social Bookmarking

What's this?

ARTICLES

Antibodies specific to acetylated histones document the existence of deposition- and transcription-related histone acetylation in Tetrahymena

R Lin, JW Leone, RG Cook and CD Allis
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030.

In this study, we have constructed synthetic peptides which are identical to hyperacetylated amino termini of two Tetrahymena core histones (tetra-acetylated H4 and penta-acetylated hv1) and used them to generate polyclonal antibodies specific for acetylated forms (mono-, di-, tri-, etc.) of these histones. Neither of these antisera recognizes histone that is unacetylated. Immunoblotting analyses demonstrate that both transcription-related and deposition-related acetate groups on H4 are recognized by both antisera. In addition, the antiserum raised against penta-acetylated hv1 also recognizes acetylated forms of this variant. Immunofluorescent analyses with both antisera demonstrate that, as expected, histone acetylation is specific to macronuclei (or new macronuclei) at all stages of the life cycle except when micronuclei undergo periods of rapid replication and chromatin assembly. During this time micronuclear staining is also detected. Our results also suggest that transcription-related acetylation begins selectively in new macronuclei immediately after the second postzygotic division. Acetylated histone is not observed in new micronuclei during stages corresponding to anlagen development and, therefore, histone acetylation can be distributed asymmetrically in development. Equally striking is the rapid turnover of acetylated histone in parental macronuclei during the time of their inactivation and elimination from the cell. Taken together, these data lend strong support to the idea that modulation of histone acetylation plays an important role in gene activation and in chromatin assembly.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Shechter, D., Nicklay, J. J., Chitta, R. K., Shabanowitz, J., Hunt, D. F., Allis, C. D. (2009). Analysis of Histones in Xenopus laevis: I. A DISTINCT INDEX OF ENRICHED VARIANTS AND MODIFICATIONS EXISTS IN EACH CELL TYPE AND IS REMODELED DURING DEVELOPMENTAL TRANSITIONS. J. Biol. Chem. 284: 1064-1074 [Abstract] [Full Text]
Andrews, K. T., Tran, T. N., Lucke, A. J., Kahnberg, P., Le, G. T., Boyle, G. M., Gardiner, D. L., Skinner-Adams, T. S., Fairlie, D. P. (2008). Potent Antimalarial Activity of Histone Deacetylase Inhibitor Analogues. Antimicrob. Agents Chemother. 52: 1454-1461 [Abstract] [Full Text]
Smith, J. J., Torigoe, S. E., Maxson, J., Fish, L. C., Wiley, E. A. (2008). A Class II Histone Deacetylase Acts on Newly Synthesized Histones in Tetrahymena. Eukaryot Cell 7: 471-482 [Abstract] [Full Text]
Cervantes, M. D., Xi, X., Vermaak, D., Yao, M.-C., Malik, H. S. (2006). The CNA1 Histone of the Ciliate Tetrahymena thermophila Is Essential for Chromosome Segregation in the Germline Micronucleus. Mol. Biol. Cell 17: 485-497 [Abstract] [Full Text]
Ivanovska, I., Khandan, T., Ito, T., Orr-Weaver, T. L. (2005). A histone code in meiosis: the histone kinase, NHK-1, is required for proper chromosomal architecture in Drosophila oocytes. Genes Dev. 19: 2571-2582 [Abstract] [Full Text]
Gurvich, N., Tsygankova, O. M., Meinkoth, J. L., Klein, P. S. (2004). Histone Deacetylase Is a Target of Valproic Acid-Mediated Cellular Differentiation. Cancer Res. 64: 1079-1086 [Abstract] [Full Text]
Pisano, C., Pratesi, G., Laccabue, D., Zunino, F., Lo Giudice, P., Bellucci, A., Pacifici, L., Camerini, B., Vesci, L., Castorina, M., Cicuzza, S., Tredici, G., Marmiroli, P., Nicolini, G., Galbiati, S., Calvani, M., Carminati, P., Cavaletti, G. (2003). Paclitaxel and Cisplatin-Induced Neurotoxicity: A Protective Role of Acetyl-L-Carnitine. Clin. Cancer Res. 9: 5756-5767 [Abstract] [Full Text]
Shen, J., Hovhannisyan, H., Lian, J. B., Montecino, M. A., Stein, G. S., Stein, J. L., van Wijnen, A. J. (2003). Transcriptional Induction of the Osteocalcin Gene During Osteoblast Differentiation Involves Acetylation of Histones H3 and H4. Mol. Endocrinol. 17: 743-756 [Abstract] [Full Text]
Lahn, B. T., Tang, Z. L., Zhou, J., Barndt, R. J., Parvinen, M., Allis, C. D., Page, D. C. (2002). Previously uncharacterized histone acetyltransferases implicated in mammalian spermatogenesis. Proc. Natl. Acad. Sci. USA 99: 8707-8712 [Abstract] [Full Text]
Shen, J., Montecino, M., Lian, J. B., Stein, G. S., van Wijnen, A. J., Stein, J. L. (2002). Histone Acetylation in Vivo at the Osteocalcin Locus Is Functionally Linked to Vitamin D-dependent, Bone Tissue-specific Transcription. J. Biol. Chem. 277: 20284-20292 [Abstract] [Full Text]
Morrison, A. J., Sardet, C., Herrera, R. E. (2002). Retinoblastoma Protein Transcriptional Repression through Histone Deacetylation of a Single Nucleosome. Mol. Cell. Biol. 22: 856-865 [Abstract] [Full Text]
Ye, Q., Hu, Y.-F., Zhong, H., Nye, A. C., Belmont, A. S., Li, R. (2001). BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations. JCB 155: 911-922 [Abstract] [Full Text]
Xu, L., Paulsen, J., Yoo, Y., Goodwin, E. B., Strome, S. (2001). Caenorhabditis elegans MES-3 Is a Target of GLD-1 and Functions Epigenetically in Germline Development. Genetics 159: 1007-1017 [Abstract] [Full Text]
Kitazono, M., Robey, R., Zhan, Z., Sarlis, N. J., Skarulis, M. C., Aikou, T., Bates, S., Fojo, T. (2001). Low Concentrations of the Histone Deacetylase Inhibitor, Depsipeptide (FR901228), Increase Expression of the Na+/I- Symporter and Iodine Accumulation in Poorly Differentiated Thyroid Carcinoma Cells. J. Clin. Endocrinol. Metab. 86: 3430-3435 [Abstract] [Full Text]
Strome, S., Powers, J., Dunn, M., Reese, K., Malone, C. J., White, J., Seydoux, G., Saxton, W. (2001). Spindle Dynamics and the Role of {gamma}-Tubulin in Early Caenorhabditis elegans Embryos. Mol. Biol. Cell 12: 1751-1764 [Abstract] [Full Text]
Sewack, G. F., Ellis, T. W., Hansen, U. (2001). Binding of TATA Binding Protein to a Naturally Positioned Nucleosome Is Facilitated by Histone Acetylation. Mol. Cell. Biol. 21: 1404-1415 [Abstract] [Full Text]
Wiley, E. A., Ohba, R., Yao, M.-C., Allis, C. D. (2000). Developmentally Regulated Rpd3p Homolog Specific to the Transcriptionally Active Macronucleus of Vegetative Tetrahymena thermophila. Mol. Cell. Biol. 20: 8319-8328 [Abstract] [Full Text]
Kim, H. S., Yoon, H., Minn, I., Park, C. B., Lee, W. T., Zasloff, M., Kim, S. C. (2000). Pepsin-Mediated Processing of the Cytoplasmic Histone H2A to Strong Antimicrobial Peptide Buforin I. J. Immunol. 165: 3268-3274 [Abstract] [Full Text]
Nikiforov, M. A., Gorovsky, M. A., Allis, C. D. (2000). A Novel Chromodomain Protein, Pdd3p, Associates with Internal Eliminated Sequences during Macronuclear Development in Tetrahymena thermophila. Mol. Cell. Biol. 20: 4128-4134 [Abstract] [Full Text]
Michael, L. F., Asahara, H., Shulman, A. I., Kraus, W. L., Montminy, M. (2000). The Phosphorylation Status of a Cyclic AMP-Responsive Activator Is Modulated via a Chromatin-Dependent Mechanism. Mol. Cell. Biol. 20: 1596-1603 [Abstract] [Full Text]
Berkowitz, L., Strome, S (2000). MES-1, a protein required for unequal divisions of the germline in early C. elegans embryos, resembles receptor tyrosine kinases and is localized to the boundary between the germline and gut cells. Development 127: 4419-4431 [Abstract]
Chen, W. Y., Townes, T. M. (2000). Molecular mechanism for silencing virally transduced genes involves histone deacetylation and chromatin condensation. Proc. Natl. Acad. Sci. USA 97: 377-382 [Abstract] [Full Text]
Gilbert, S. L., Sharp, P. A. (1999). Promoter-specific hypoacetylation of X-inactivated genes. Proc. Natl. Acad. Sci. USA 96: 13825-13830 [Abstract] [Full Text]
Nikiforov, M. A., Smothers, J. F., Gorovsky, M. A., Allis, C. D. (1999). Excision of micronuclear-specific DNA requires parental expression of Pdd2p andoccursindependentlyfromDNA replication in Tetrahymena thermophila. Genes Dev. 13: 2852-2862 [Abstract] [Full Text]
Sherman, J. M., Stone, E. M., Freeman-Cook, L. L., Brachmann, C. B., Boeke, J. D., Pillus, L. (1999). The Conserved Core of a Human SIR2 Homologue Functions in Yeast Silencing. Mol. Biol. Cell 10: 3045-3059 [Abstract] [Full Text]
Waterborg, J. H. (1998). Dynamics of Histone Acetylation in Chlamydomonas reinhardtii. J. Biol. Chem. 273: 27602-27609 [Abstract] [Full Text]
Hendzel, M. J., Nishioka, W. K., Raymond, Y., Allis, C. D., Bazett-Jones, D. P., Th'ng, J. P.�H. (1998). Chromatin Condensation Is Not Associated with Apoptosis. J. Biol. Chem. 273: 24470-24478 [Abstract] [Full Text]
Pikaart, M. J., Recillas-Targa, F., Felsenfeld, G. (1998). Loss of transcriptional activity of a transgene is accompanied by DNA methylation and histone deacetylation and is prevented by insulators. Genes Dev. 12: 2852-2862 [Abstract] [Full Text]
Howe, L., Ranalli, T. A., Allis, C. D., Ausio, J. (1998). Transcriptionally Active Xenopus laevis Somatic 5�S Ribosomal RNA Genes Are Packaged with Hyperacetylated Histone H4, Whereas Transcriptionally Silent Oocyte Genes Are Not. J. Biol. Chem. 273: 20693-20696 [Abstract] [Full Text]
Wei, Y., Mizzen, C. A., Cook, R. G., Gorovsky, M. A., Allis, C. D. (1998). Phosphorylation of histone H3 at serine 10�is correlated with chromosome condensation during mitosis and meiosis in Tetrahymena. Proc. Natl. Acad. Sci. USA 95: 7480-7484 [Abstract] [Full Text]
Walia, H., Chen, H. Y., Sun, J.-M., Holth, L. T., Davie, J. R. (1998). Histone Acetylation Is Required to Maintain the Unfolded Nucleosome Structure Associated with Transcribing DNA. J. Biol. Chem. 273: 14516-14522 [Abstract] [Full Text]
EDMONDSON, D.G., ZHANG, W., WATSON, A., XU, W., BONE, J.R., YU, Y., STILLMAN, D., ROTH, S.Y. (1998). In Vivo Functions of Histone Acetylation/Deacetylation in Tup1p Repression and Gcn5p Activation. Cold Spring Harb Symp Quant Biol 63: 459-468 [Abstract]
Sheridan, P. L., Mayall, T. P., Verdin, E., Jones, K. A. (1997). Histone acetyltransferases regulate HIV-1 enhancer activity in�vitro. Genes Dev. 11: 3327-3340 [Abstract] [Full Text]
Monson, E. K., de Bruin, D., Zakian, V. A. (1997). The yeast Cac1 protein is required for the stable inheritance of transcriptionally repressed chromatin at�telomeres. Proc. Natl. Acad. Sci. USA 94: 13081-13086 [Abstract] [Full Text]
Jahn, C.�L., Ling, Z., Tebeau, C.�M., Klobutcher, L.�A. (1997). An unusual histone H3 specific for early macronuclear development in Euplotes�crassus. Proc. Natl. Acad. Sci. USA 94: 1332-1337 [Abstract] [Full Text]
Sweet, M. T., Carlson, G., Cook, R. G., Nelson, D., Allis, C. D. (1997). Phosphorylation of Linker Histones by a Protein Kinase A-like Activity in Mitotic Nuclei. J. Biol. Chem. 272: 916-923 [Abstract] [Full Text]
Brachmann, C B, Sherman, J M, Devine, S E, Cameron, E E, Pillus, L, Boeke, J D (1995). The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression, and chromosome stability.. Genes Dev. 9: 2888-2902 [Abstract]
Megee, P C, Morgan, B A, Smith, M M (1995). Histone H4 and the maintenance of genome integrity.. Genes Dev. 9: 1716-1727 [Abstract]
Thompson, E., Legouy, E, Christians, E, Renard, J. (1995). Progressive maturation of chromatin structure regulates HSP70.1 gene expression in the preimplantation mouse embryo. Development 121: 3425-3437 [Abstract]
Worrad, D., Turner, B., Schultz, R. (1995). Temporally restricted spatial localization of acetylated isoforms of histone H4 and RNA polymerase II in the 2-cell mouse embryo. Development 121: 2949-2959 [Abstract]
Kapler, G M, Blackburn, E H (1994). A weak germ-line excision mutation blocks developmentally controlled amplification of the rDNA minichromosome of Tetrahymena thermophila.. Genes Dev. 8: 84-95 [Abstract]
Stargell, L A, Bowen, J, Dadd, C A, Dedon, P C, Davis, M, Cook, R G, Allis, C D, Gorovsky, M A (1993). Temporal and spatial association of histone H2A variant hv1 with transcriptionally competent chromatin during nuclear development in Tetrahymena thermophila.. Genes Dev. 7: 2641-2651 [Abstract]
Braunstein, M, Rose, A B, Holmes, S G, Allis, C D, Broach, J R (1993). Transcriptional silencing in yeast is associated with reduced nucleosome acetylation.. Genes Dev. 7: 592-604 [Abstract]
Thompson, J.S., Hecht, A., Grunstein, M. (1993). Histones and the Regulation of Heterochromatin in Yeast. Cold Spring Harb Symp Quant Biol 58: 247-256 [Abstract]
Lin, R, Cook, R G, Allis, C D (1991). Proteolytic removal of core histone amino termini and dephosphorylation of histone H1 correlate with the formation of condensed chromatin and transcriptional silencing during Tetrahymena macronuclear development.. Genes Dev. 5: 1601-1610 [Abstract]
Kruhlak, M. J., Hendzel, M. J., Fischle, W., Bertos, N. R., Hameed, S., Yang, X.-J., Verdin, E., Bazett-Jones, D. P. (2001). Regulation of Global Acetylation in Mitosis through Loss of Histone Acetyltransferases and Deacetylases from Chromatin. J. Biol. Chem. 276: 38307-38319 [Abstract] [Full Text]