Syndecan and tenascin expression is induced by epithelial-mesenchymal interactions in embryonic tooth mesenchyme

doi:10.1083/jcb.108.5.1945

This Article

	Full Text (PDF, 3098K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Vainio, S.
	Articles by Thesleff, I.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Vainio, S.
	Articles by Thesleff, I.


Social Bookmarking

	What's this?

ARTICLES

Syndecan and tenascin expression is induced by epithelial-mesenchymal interactions in embryonic tooth mesenchyme

S Vainio, M Jalkanen and I Thesleff
Department of Pedodontics and Orthodontics, University of Helsinki, Finland.

Morphogenesis of embryonic organs is regulated by epithelial- mesenchymal interactions associating with changes in the extracellular matrix (ECM). The response of the cells to the changes in the ECM must involve integral cell surface molecules that recognize their matrix ligand and initiate transmission of signal intracellularly. We have studied the expression of the cell surface proteoglycan, syndecan, which is a matrix receptor for epithelial cells (Saunders, S., M. Jalkanen, S. O'Farrell, and M. Bernfield. J. Cell Biol. In press.), and the matrix glycoprotein, tenascin, which has been proposed to be involved in epithelial-mesenchymal interactions (Chiquet-Ehrismann, R., E. J. Mackie, C. A. Pearson, and T. Sakakura. 1986. Cell. 47:131-139) in experimental tissue recombinations of dental epithelium and mesenchyme. Our earlier studies have shown that in mouse embryos both syndecan and tenascin are intensely expressed in the condensing dental mesenchyme surrounding the epithelial bud (Thesleff, I., M. Jalkanen, S. Vainio, and M. Bernfield. 1988. Dev. Biol. 129:565-572; Thesleff, I., E. Mackie, S. Vainio, and R. Chiquet-Ehrismann. 1987. Development. 101:289-296). Analysis of rat-mouse tissue recombinants by a monoclonal antibody against the murine syndecan showed that the presumptive dental epithelium induces the expression of syndecan in the underlying mesenchyme. The expression of tenascin was induced in the dental mesenchyme in the same area as syndecan. The syndecan and tenascin positive areas increased with time of epithelial-mesenchymal contact. Other ECM molecules, laminin, type III collagen, and fibronectin, did not show a staining pattern similar to that of syndecan and tenascin. Oral epithelium from older embryos had lost its ability to induce syndecan expression but the presumptive dental epithelium induced syndecan expression even in oral mesenchyme of older embryos. Our results indicate that the expression of syndecan and tenascin in the tooth mesenchyme is regulated by epithelial-mesenchymal interactions. Because of their early appearance, syndecan and tenascin may be used to study the molecular regulation of this interaction. The similar distribution patterns of syndecan and tenascin in vivo and in vitro and their early appearance as a result of epithelial-mesenchymal interaction suggest that these molecules may be involved in the condensation and differentiation of dental mesenchymal cells.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Jang, J.-H., Hwang, J.-H., Chung, C.-P., Choung, P.-H. (2004). Identification and Kinetics Analysis of a Novel Heparin-binding Site (KEDK) in Human Tenascin-C. J. Biol. Chem. 279: 25562-25566 [Abstract] [Full Text]
Maeda, T., Alexander, C. M., Friedl, A. (2004). Induction of Syndecan-1 Expression in Stromal Fibroblasts Promotes Proliferation of Human Breast Cancer Cells. Cancer Res. 64: 612-621 [Abstract] [Full Text]
Oksjoki, S., Sallinen, S., Vuorio, E., Anttila, L. (1999). Cyclic expression of mRNA transcripts for connective tissue components in the mouse ovary. Mol Hum Reprod 5: 803-808 [Abstract] [Full Text]
Nakamura, H., Ozawa, H. (1997). Immunolocalization of CD44 and the Ezrin-Radixin-Moesin (ERM) Family in the Stratum Intermedium and Papillary Layer of the Mouse Enamel Organ. J. Histochem. Cytochem. 45: 1481-1492 [Abstract] [Full Text]
Schuger, L., Skubitz, A. P.N., Zhang, J., Sorokin, L., He, L. (1997). Laminin {alpha}1 Chain Synthesis in the Mouse Developing Lung: Requirement for Epithelial-Mesenchymal Contact and Possible Role in Bronchial Smooth muscle Development. JCB 139: 553-562 [Abstract] [Full Text]
Maas, R., Bei, M. (1997). The Genetic Control of Early Tooth Development. CROBM 8: 4-39 [Abstract] [Full Text]
Kainulainen, V., Nelimarkka, L., Jarvelainen, H., Laato, M., Jalkanen, M., Elenius, K. (1996). Suppression of Syndecan-1 Expression in Endothelial Cells by Tumor Necrosis Factor-alpha. J. Biol. Chem. 271: 18759-18766 [Abstract] [Full Text]
Kratochwil, K, Dull, M, Farinas, I, Galceran, J, Grosschedl, R (1996). Lef1 expression is activated by BMP-4 and regulates inductive tissue interactions in tooth and hair development.. Genes Dev. 10: 1382-1394 [Abstract]
Tabata, M., Kim, K, Liu, J., Yamashita, K, Matsumura, T, Kato, J, Iwamoto, M, Wakisaka, S, Matsumoto, K, Nakamura, T, Kumegawa, M, Kurisu, K (1996). Hepatocyte growth factor is involved in the morphogenesis of tooth germ in murine molars. Development 122: 1243-1251 [Abstract]
Lukinmaa, P.-L., Allemanni, G., Waltimo, J., Zardi, L. (1996). Immunoreactivity of Tenascin-C in Dentin Matrix in Dentinogenesis Imperfecta Associated with Osteogenesis Imperfecta. JDR 75: 581-587 [Abstract]
Stanley, M. J., Liebersbach, B. F., Liu, W., Anhalt, D. J., Sanderson, R. D. (1995). Heparan Sulfate-mediated Cell Aggregation. J. Biol. Chem. 270: 5077-5083 [Abstract] [Full Text]
Fischer, D., Chiquet-Ehrismann, R., Bernasconi, C., Chiquet, M. (1995). A Single Heparin Binding Region within the Fibrinogen-like Domain Is Functional in Chick Tenascin-C. J. Biol. Chem. 270: 3378-3384 [Abstract] [Full Text]
Weil, M, Itin, A, Keshet, E (1995). A role for mesenchyme-derived tachykinins in tooth and mammary gland morphogenesis. Development 121: 2419-2428 [Abstract]
Wirl, G, Hermann, M, Ekblom, P, Fassler, R (1995). Mammary epithelial cell differentiation in vitro is regulated by an interplay of EGF action and tenascin-C downregulation. J. Cell Sci. 108: 2445-2456 [Abstract]
Itoh, K., Sokol, S. Y. (1994). Heparan sulfate proteoglycans are required for mesoderm formation in Xenopus embryos. Development 120: 2703-2711 [Abstract]
Nieminen, P., Vainio, S., Jernvall, J., Lukinmaa, P.-L., Lehtonen, E., Thesleff, I. (1993). A Chondroitin Sulfate Epitope in Mammalian Dental Pulp and its Developmental Expression in Mouse Dental Papilla. JDR 72: 1460-1472 [Abstract]
du Cros, D., Isaacs, K, Moore, G. (1993). Distribution of acidic and basic fibroblast growth factors in ovine skin during follicle morphogenesis. J. Cell Sci. 105: 667-674 [Abstract]
Jowett, A., Vainio, S, Ferguson, M., Sharpe, P., Thesleff, I (1993). Epithelial-mesenchymal interactions are required for msx 1 and msx 2 gene expression in the developing murine molar tooth. Development 117: 461-470 [Abstract]
Lukinmaa, P.-L., Mackie, E.J., Thesleff, I. (1991). Immunohistochemical Localization of the Matrix Glycoproteins-Tenascin and the ED-sequence-containing Form of Cellular Fibronectin--in Human Permanent Teeth and Periodontal Ligament. JDR 70: 19-26 [Abstract]