Primary structure analysis of an integral membrane glycoprotein of the nuclear pore

doi:10.1083/jcb.108.6.2083

This Article

	Full Text (PDF, 1508K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Wozniak, R. W.
	Articles by Blobel, G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Wozniak, R. W.
	Articles by Blobel, G.


Social Bookmarking

	What's this?

ARTICLES

Primary structure analysis of an integral membrane glycoprotein of the nuclear pore

RW Wozniak, E Bartnik and G Blobel
Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York 10021.

The complete primary structure of an integral membrane glycoprotein of the nuclear pore was deduced from the cDNA sequence. The cDNA encodes a polypeptide of 204,205 D containing a 25-residue-long signal sequence, two hydrophobic segments that could function as transmembrane segments, and 13 potential N-linked oligosaccharide addition sites. Endoglycosidase H reduces the molecular mass by approximately 9 kD suggesting that not all of these 13 sites are used. We discuss possible models for the topology of this protein in the pore membrane as well as a possible role in the formation of pores and pore complexes.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Rasala, B. A., Ramos, C., Harel, A., Forbes, D. J. (2008). Capture of AT-rich Chromatin by ELYS Recruits POM121 and NDC1 to Initiate Nuclear Pore Assembly. Mol. Biol. Cell 19: 3982-3996 [Abstract] [Full Text]
Salpingidou, G., Rzepecki, R., Kiseleva, E., Lyon, C., Lane, B., Fusiek, K., Golebiewska, A., Drummond, S., Allen, T., Ellis, J. A., Smythe, C., Goldberg, M. W., Hutchison, C. J. (2008). NEP-A and NEP-B both contribute to nuclear pore formation in Xenopus eggs and oocytes. J. Cell Sci. 121: 706-716 [Abstract] [Full Text]
Vaillant, D. C., Paulin-Levasseur, M. (2008). Evaluation of Mammalian Cell-free Systems of Nuclear Disassembly and Assembly. J. Histochem. Cytochem. 56: 157-173 [Abstract] [Full Text]
Broers, J. L. V., Ramaekers, F. C. S., Bonne, G., Yaou, R. B., Hutchison, C. J. (2006). Nuclear lamins: laminopathies and their role in premature ageing.. Physiol. Rev. 86: 967-1008 [Abstract] [Full Text]
Stavru, F., Nautrup-Pedersen, G., Cordes, V. C., Gorlich, D. (2006). Nuclear pore complex assembly and maintenance in POM121- and gp210-deficient cells. JCB 173: 477-483 [Abstract] [Full Text]
Miao, M., Ryan, K. J., Wente, S. R. (2006). The Integral Membrane Protein Pom34p Functionally Links Nucleoporin Subcomplexes. Genetics 172: 1441-1457 [Abstract] [Full Text]
Patre, M., Tabbert, A., Hermann, D., Walczak, H., Rackwitz, H.-R., Cordes, V. C., Ferrando-May, E. (2006). Caspases Target Only Two Architectural Components within the Core Structure of the Nuclear Pore Complex. J. Biol. Chem. 281: 1296-1304 [Abstract] [Full Text]
Belanger, K. D., Gupta, A., MacDonald, K. M., Ott, C. M., Hodge, C. A., Cole, C. M., Davis, L. I. (2005). Nuclear Pore Complex Function in Saccharomyces cerevisiae Is Influenced by Glycosylation of the Transmembrane Nucleoporin Pom152p. Genetics 171: 935-947 [Abstract] [Full Text]
Hawryluk-Gara, L. A., Shibuya, E. K., Wozniak, R. W. (2005). Vertebrate Nup53 Interacts with the Nuclear Lamina and Is Required for the Assembly of a Nup93-containing Complex. Mol. Biol. Cell 16: 2382-2394 [Abstract] [Full Text]
Drummond, S. P., Wilson, K. L. (2002). Interference with the cytoplasmic tail of gp210 disrupts "close apposition" of nuclear membranes and blocks nuclear pore dilation. JCB 158: 53-62 [Abstract] [Full Text]
Kihlmark, M., Imreh, G., Hallberg, E. (2002). Sequential degradation of proteins from the nuclear envelope during apoptosis. J. Cell Sci. 114: 3643-3653 [Abstract] [Full Text]
Cikaluk, D. E., Tahbaz, N., Hendricks, L. C., DiMattia, G. E., Hansen, D., Pilgrim, D., Hobman, T. C. (1999). GERp95, a Membrane-associated Protein that Belongs to a Family of Proteins Involved in Stem Cell Differentiation. Mol. Biol. Cell 10: 3357-3372 [Abstract] [Full Text]
Tcheperegine, S. E., Marelli, M., Wozniak, R. W. (1999). Topology and Functional Domains of the Yeast Pore Membrane Protein Pom152p. J. Biol. Chem. 274: 5252-5258 [Abstract] [Full Text]
Bodoor, K, Shaikh, S, Salina, D, Raharjo, W., Bastos, R, Lohka, M, Burke, B (1999). Sequential recruitment of NPC proteins to the nuclear periphery at the end of mitosis. J. Cell Sci. 112: 2253-2264 [Abstract]
Buendia, B, Santa-Maria, A, Courvalin, J. (1999). Caspase-dependent proteolysis of integral and peripheral proteins of nuclear membranes and nuclear pore complex proteins during apoptosis. J. Cell Sci. 112: 1743-1753 [Abstract]
Love, D. C., Sweitzer, T. D., Hanover, J. A. (1998). Reconstitution of HIV-1 Rev nuclear export: Independent requirements for nuclear import and export. Proc. Natl. Acad. Sci. USA 95: 10608-10613 [Abstract] [Full Text]
Bucci, M., Wente, S. R. (1998). A Novel Fluorescence-based Genetic Strategy Identifies Mutants of Saccharomyces cerevisiae Defective for Nuclear Pore Complex Assembly. Mol. Biol. Cell 9: 2439-2461 [Abstract] [Full Text]
Heese-Peck, A., Raikhel, N. V. (1998). A Glycoprotein Modified with Terminal N-Acetylglucosamine and Localized at the Nuclear Rim Shows Sequence Similarity to Aldose-1-Epimerases. Plant Cell 10: 599-612 [Abstract] [Full Text]
Ho, A. K., Raczniak, G. A., Ives, E. B., Wente, S. R. (1998). The Integral Membrane Protein Snl1p Is Genetically Linked to Yeast Nuclear Pore Complex Function. Mol. Biol. Cell 9: 355-373 [Abstract] [Full Text]
Yang, L., Guan, T., Gerace, L. (1997). Integral Membrane Proteins of the Nuclear Envelope Are Dispersed throughout the Endoplasmic Reticulum during Mitosis. JCB 137: 1199-1210 [Abstract] [Full Text]
Yang, H., Lu, D., Vinson, G. P., Raizada, M. K. (1997). Involvement of MAP Kinase in Angiotensin II-Induced Phosphorylation and Intracellular Targeting of Neuronal AT1 Receptors. J. Neurosci. 17: 1660-1669 [Abstract] [Full Text]
Cordes, V. C., Reidenbach, S., Rackwitz, H.-R., Franke, W. W. (1997). Identification of Protein p270/Tpr as a Constitutive Component of the Nuclear Pore Complex-attached Intranuclear Filaments. JCB 136: 515-529 [Abstract] [Full Text]
Goldberg, M., Wiese, C, Allen, T., Wilson, K. (1997). Dimples, pores, star-rings, and thin rings on growing nuclear envelopes: evidence for structural intermediates in nuclear pore complex assembly. J. Cell Sci. 110: 409-420 [Abstract]
Ewald, A, Kossner, U, Scheer, U, Dabauvalle, M. (1996). A biochemical and immunological comparison of nuclear and cytoplasmic pore complexes. J. Cell Sci. 109: 1813-1824 [Abstract]
Buss, F, Kent, H, Stewart, M, Bailer, S., Hanover, J. (1994). Role of different domains in the self-association of rat nucleoporin p62. J. Cell Sci. 107: 631-638 [Abstract]
Amberg, D C, Goldstein, A L, Cole, C N (1992). Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA.. Genes Dev. 6: 1173-1189 [Abstract]