A calsequestrin-like protein in the endoplasmic reticulum of the sea urchin: localization and dynamics in the egg and first cell cycle embryo

doi:10.1083/jcb.109.1.149

This Article

	Full Text (PDF, 6498K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Henson, J. H.
	Articles by Kaminer, B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Henson, J. H.
	Articles by Kaminer, B.


Social Bookmarking

	What's this?

ARTICLES

A calsequestrin-like protein in the endoplasmic reticulum of the sea urchin: localization and dynamics in the egg and first cell cycle embryo

JH Henson, DA Begg, SM Beaulieu, DJ Fishkind, EM Bonder, M Terasaki, D Lebeche and B Kaminer
Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, Massachusetts 02115.

Using an antiserum produced against a purified calsequestrin-like (CSL) protein from a microsomal fraction of sea urchin eggs, we performed light and electron microscopic immunocytochemical localizations on sea urchin eggs and embryos in the first cell cycle. The sea urchin CSL protein has been found to bind Ca++ similarly to calsequestrin, the well-characterized Ca++ storage protein in the sarcoplasmic reticulum of muscle cells. In semi-thin frozen sections of unfertilized eggs, immunofluorescent staining revealed a tubuloreticular network throughout the cytoplasm. Staining of isolated egg cortices with the CSL protein antiserum showed the presence of a submembranous polygonal, tubular network similar to ER network patterns seen in other cells and in egg cortices treated with the membrane staining dye DiIC16[3]. In frozen sections of embryos during interphase of the first cell cycle, a cytoplasmic network similar to that of the unfertilized egg was present. During mitosis, we observed a dramatic concentration of the antibody staining within the asters of the mitotic apparatus where ER is known to aggregate. Electron microscopic localization on unfertilized eggs using peroxidase-labeled secondary antibody demonstrated the presence of the CSL protein within the luminal compartment of ER-like tubules. Finally, in frozen sections of centrifugally stratified eggs, the immunofluorescent staining concentrated in the clear zone: a layer highly enriched in ER and thought to be the site of calcium release upon fertilization. This localization of a CSL protein within the ER of the egg provides evidence for the ability of this organelle to serve a Ca++ storage role in the regulation of intracellular Ca++ in nonmuscle cells in general, and in the regulation of fertilization and cell division in sea urchin eggs in particular.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Whitaker, M. (2008). Calcium signalling in early embryos. Phil Trans R Soc B 363: 1401-1418 [Abstract] [Full Text]
McCarron, J. G., Olson, M. L. (2008). A Single Luminally Continuous Sarcoplasmic Reticulum with Apparently Separate Ca2+ Stores in Smooth Muscle. J. Biol. Chem. 283: 7206-7218 [Abstract] [Full Text]
Browne, C. L., Swan, J. B., Rankin, E. E., Calvert, H., Griffiths, S., Tytell, M. (2007). Extracellular heat shock protein 70 has novel functional effects on sea urchin eggs and coelomocytes. J. Exp. Biol. 210: 1275-1287 [Abstract] [Full Text]
Whitaker, M. (2006). Calcium at Fertilization and in Early Development. Physiol. Rev. 86: 25-88 [Abstract] [Full Text]
Otsuki, J., Okada, A., Morimoto, K., Nagai, Y., Kubo, H. (2004). The relationship between pregnancy outcome and smooth endoplasmic reticulum clusters in MII human oocytes. Hum Reprod 19: 1591-1597 [Abstract] [Full Text]
Du, Y., Ferro-Novick, S., Novick, P. (2004). Dynamics and inheritance of the endoplasmic reticulum. J. Cell Sci. 117: 2871-2878 [Abstract] [Full Text]
Pichler, S., Gonczy, P., Schnabel, H., Pozniakowski, A., Ashford, A., Schnabel, R., Hyman, A. A. (2000). OOC-3, a novel putative transmembrane protein required for establishment of cortical domains and spindle orientation in the P(1) blastomere of C. elegans embryos. Development 127: 2063-2073 [Abstract]
Terasaki, M. (2000). Dynamics of the Endoplasmic Reticulum and Golgi Apparatus during Early Sea Urchin Development. Mol. Biol. Cell 11: 897-914 [Abstract] [Full Text]
Foe, V., Field, C., Odell, G. (2000). Microtubules and mitotic cycle phase modulate spatiotemporal distributions of F-actin and myosin II in Drosophila syncytial blastoderm embryos. Development 127: 1767-1787 [Abstract]
Lucero, H. A., Kaminer, B. (1999). The Role of Calcium on the Activity of ERcalcistorin/Protein-disulfide Isomerase and the Significance of the C-terminal and Its Calcium Binding. A COMPARISON WITH MAMMALIAN PROTEIN-DISULFIDE ISOMERASE. J. Biol. Chem. 274: 3243-3251 [Abstract] [Full Text]
Becker, K., Hart, N. (1999). Reorganization of filamentous actin and myosin-II in zebrafish eggs correlates temporally and spatially with cortical granule exocytosis. J. Cell Sci. 112: 97-110 [Abstract]
Lucero, H. A., Lebeche, D., Kaminer, B. (1998). ERcalcistorin/Protein-disulfide Isomerase Acts as a Calcium Storage Protein in the Endoplasmic Reticulum of a Living Cell. COMPARISON WITH CALRETICULIN AND CALSEQUESTRIN. J. Biol. Chem. 273: 9857-9863 [Abstract] [Full Text]
Terasaki, M., Miyake, K., McNeil, P. L. (1997). Large Plasma Membrane Disruptions Are Rapidly Resealed by Ca2+-dependent Vesicle-Vesicle Fusion Events. JCB 139: 63-74 [Abstract] [Full Text]
Fishkind, D., Silverman, J., Wang, Y. (1996). Function of spindle microtubules in directing cortical movement and actin filament organization in dividing cultured cells. J. Cell Sci. 109: 2041-2051 [Abstract]
Terasaki, M (1995). Visualization of exocytosis during sea urchin egg fertilization using confocal microscopy. J. Cell Sci. 108: 2293-2300 [Abstract]
Stelly, N, Halpern, S, Nicolas, G, Fragu, P, Adoutte, A (1995). Direct visualization of a vast cortical calcium compartment in Paramecium by secondary ion mass spectrometry (SIMS) microscopy: possible involvement in exocytosis. J. Cell Sci. 108: 1895-1909 [Abstract]
Mabuchi, I. (1994). Cleavage furrow: timing of emergence of contractile ring actin filaments and establishment of the contractile ring by filament bundling in sea urchin eggs. J. Cell Sci. 107: 1853-1862 [Abstract]
Callaini, G, Riparbelli, M., Dallai, R (1994). The distribution of cytoplasmic bacteria in the early Drosophila embryo is mediated by astral microtubules. J. Cell Sci. 107: 673-682 [Abstract]
Barton, N. R., Bonder, E. M., Fishkind, D. J., Warren, R. H., Pratt, M. M. (1992). A novel vesicle-associated protein (VAP-1) in sea urchin eggs containing multiple RNA-binding consensus sequences. J. Cell Sci. 103: 797-809 [Abstract]
Picard, A, Cavadore, J., Lory, P, Bernengo, J., Ojeda, C, Doree, M (1990). Microinjection of a conserved peptide sequence of p34cdc2 induces a Ca2+ transient in oocytes. Science 247: 327-329 [Abstract]