Expression of extra domain A fibronectin sequence in vascular smooth muscle cells is phenotype dependent

doi:10.1083/jcb.109.1.357

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Expression of extra domain A fibronectin sequence in vascular smooth muscle cells is phenotype dependent

MA Glukhova, MG Frid, BV Shekhonin, TD Vasilevskaya, J Grunwald, M Saginati and VE Koteliansky
Institute of Experimental Cardiology, Academy of Medical Sciences, Moscow, Union of Soviet Socialist Republics.

Different fibronectin (FN) variants arise from the single gene transcript alternatively spliced in a tissue-specific manner (Hynes, R. O. 1985. Annu. Rev. Cell Biol. 1:67-90; Owens, R. J., A. R. Kornblihtt, and F. E. Baralle. 1986. Oxf. Surv. Eurcaryotic Genes. 3:141-160). We used mAb IST-9, specific for extra domain A (ED-A) FN sequence, and cDNA probe to ED-A exon to determine whether ED-A is present in FN synthesized by vascular smooth muscle cells (SMCs) and, if so, whether expression of ED-A is SMC phenotype dependent. ED-A-containing FN (A- FN) was not revealed in tunica media of human arteries and normal rat aorta by immunofluorescence and immunoblotting techniques. A cDNA probe to ED-A exon did not hybridize with RNA isolated from human aortic media. A positive reaction with IST-9 was observed in (a) diffuse intimal thickening and atherosclerotic plaque from human arteries; (b) experimentally induced intimal thickening in rat aorta; and (c) cultured vascular SMCs. A-FN mRNA was present in the RNA preparation from human aortic intima as judged by hybridization with cDNA probe to ED-A. On the other hand, an mAb interacting with an epitope common for all FN variants revealed FN in both intima and media of human arteries and in the normal rat aorta. A cDNA probe to a sequence shared by all FN variants hybridized with RNA from both intima and media of human aorta, though the level of expression was higher in intima. The data suggest that ED-A exon is omitted during splicing of the FN mRNA precursor in medial SMCs while the expression of A-FN is characteristic of "modulated" SMCs--those of intimal thickenings, of atherosclerotic lesions, and growing in culture.
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Zuk, A., Bonventre, J. V., Matlin, K. S. (2001). Expression of fibronectin splice variants in the postischemic rat kidney. Am. J. Physiol. Renal Physiol. 280: F1037-F1053 [Abstract] [Full Text]
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Liao, Y.-F., Wieder, K. G., Classen, J. M., De Water, L. V. (1999). Identification of Two Amino Acids within the EIIIA (ED-A) Segment of Fibronectin Constituting the Epitope for Two Function-blocking Monoclonal Antibodies. J. Biol. Chem. 274: 17876-17884 [Abstract] [Full Text]
Serini, G., Bochaton-Piallat, M.-L., Ropraz, P., Geinoz, A., Borsi, L., Zardi, L., Gabbiani, G. (1998). The Fibronectin Domain ED-A Is Crucial for Myofibroblastic Phenotype Induction by Transforming Growth Factor-{beta}1. JCB 142: 873-881 [Abstract] [Full Text]
Bezie, Y., Lamaziere, J.-M. D., Laurent, S., Challande, P., Cunha, R. S., Bonnet, J., Lacolley, P. (1998). Fibronectin Expression and Aortic Wall Elastic Modulus in Spontaneously Hypertensive Rats. Arterioscler. Thromb. Vasc. Bio. 18: 1027-1034 [Abstract] [Full Text]
George, E. L., Baldwin, H. S., Hynes, R. O. (1997). Fibronectins Are Essential for Heart and Blood Vessel Morphogenesis But Are Dispensable for Initial Specification of Precursor Cells. Blood 90: 3073-3081 [Abstract] [Full Text]
Thyberg, J., Blomgren, K., Roy, J., Tran, P. K., Hedin, U. (1997). Phenotypic Modulation of Smooth Muscle Cells after Arterial Injury Is Associated with Changes in the Distribution of Laminin and Fibronectin. J. Histochem. Cytochem. 45: 837-846 [Abstract] [Full Text]
Hungerford, J. E., Hoeffler, J. P., Bowers, C. W., Dahm, L. M., Falchetto, R., Shabanowitz, J., Hunt, D. F., Little, C. D. (1997). Identification of a Novel Marker for Primordial Smooth Muscle and Its Differential Expression Pattern in Contractile vs Noncontractile Cells. JCB 137: 925-937 [Abstract] [Full Text]
Sabri, A., Levy, B. I., Poitevin, P., Caputo, L., Faggin, E., Marotte, F., Rappaport, L., Samuel, J. L. (1997). Differential Roles of AT1 and AT2 Receptor Subtypes in Vascular Trophic and Phenotypic Changes in Response to Stimulation With Angiotensin II. Arterioscler. Thromb. Vasc. Bio. 17: 257-264 [Abstract] [Full Text]
Belknap, J. K., Grieshaber, N. A., Schwartz, P. E., Orton, E. C., Reidy, M. A., Majack, R. A. (1996). Tropoelastin Gene Expression in Individual Vascular Smooth Muscle Cells : Relationship to DNA Synthesis During Vascular Development and After Arterial Injury. Circ. Res. 78: 388-394 [Abstract] [Full Text]
Sivaram, P., Obunike, J. C., Goldberg, I. J. (1995). Lysolecithin-induced Alteration of Subendothelial Heparan Sulfate Proteoglycans Increases Monocyte Binding to Matrix. J. Biol. Chem. 270: 29760-29765 [Abstract] [Full Text]
Dubin, D., Peters, J. H., Brown, L. F., Logan, B., Kent, K. C., Berse, B., Berven, S., Cercek, B., Sharifi, B. G., Pratt, R. E., Dzau, V. J., Van De Water, L. (1995). Balloon Catheterization Induces Arterial Expression of Embryonic Fibronectins. Arterioscler. Thromb. Vasc. Bio. 15: 1958-1967 [Abstract] [Full Text]
Schwartz, S. M., deBlois, D., O'Brien, E. R. M. (1995). The Intima : Soil for Atherosclerosis and Restenosis. Circ. Res. 77: 445-465 [Full Text]
Bauters, C., Marotte, F., Hamon, M., Oliviero, P., Farhadian, F., Robert, V., Samuel, J. L., Rappaport, L. (1995). Accumulation of Fetal Fibronectin mRNAs After Balloon Denudation of Rabbit Arteries. Circulation 92: 904-911 [Abstract] [Full Text]
Gibbons, G. H., Dzau, V. J. (1994). The Emerging Concept of Vascular Remodeling. NEJM 330: 1431-1438 [Full Text]
Hynes, R.O., George, E.L., Georges, E.N., Guan, J.-L., Rayburn, H., Yang, J.T. (1992). Toward a Genetic Analysis of Cell-Matrix Adhesion. Cold Spring Harb Symp Quant Biol 57: 249-258 [Abstract]