Mitotic Golgi fragments in HeLa cells and their role in the reassembly pathway

doi:10.1083/jcb.109.2.463

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Mitotic Golgi fragments in HeLa cells and their role in the reassembly pathway

JM Lucocq, EG Berger and G Warren
Department of Biochemistry, University of Dundee, Scotland.

Immunoelectron microscopy and stereology were used to identify and quantitate Golgi fragments in metaphase HeLa cells and to study Golgi reassembly during telophase. On ultrathin frozen sections of metaphase cells, labeling for the Golgi marker protein, galactosyltransferase, was found over multivesicular Golgi clusters and free vesicles that were found mainly in the mitotic spindle region. The density of Golgi cluster membrane varied from cell to cell and was inversely related to the density of free vesicles in the spindle. There were thousands of free Golgi vesicles and they comprised a significant proportion of the total Golgi membrane. During telophase, the distribution of galactosyltransferase labeling shifted from free Golgi vesicles towards Golgi clusters and the population of free vesicles was depleted. The number of clusters was no more than in metaphase cells so the observed fourfold increase in membrane surface meant that individual clusters had increased in size. More than half of these had cisterna(e) and were located next to "buds" on the endoplasmic reticulum. Early in G1 the number of clusters dropped as they congregated in the juxtanuclear region and fused. These results show that fragmentation of the Golgi apparatus yields Golgi clusters and free vesicles and reassembly from these fragments is at least a two-step process: (a) growth of a limited number of dispersed clusters by accretion and fusion of vesicles to form cisternal clusters next to membranous "buds" on the endoplasmic reticulum; (b) congregation and fusion to form the interphase Golgi stack in the juxtanuclear region.
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Lesa, G. M., Seemann, J., Shorter, J., Vandekerckhove, J., Warren, G. (2000). The Amino-terminal Domain of the Golgi Protein Giantin Interacts Directly with the Vesicle-tethering Protein p115. J. Biol. Chem. 275: 2831-2836 [Abstract] [Full Text]
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Coupin, G., Muller, C., Remy-Kristensen, A, Kuhry, J. (1999). Cell surface membrane homeostasis and intracellular membrane traffic balance in mouse L929 cells. J. Cell Sci. 112: 2431-2440 [Abstract]
Farmaki, T, Ponnambalam, S, Prescott, A., Clausen, H, Tang, B., Hong, W, Lucocq, J. (1999). Forward and retrograde trafficking in mitotic animal cells. ER-Golgi transport arrest restricts protein export from the ER into COPII-coated structures. J. Cell Sci. 112: 589-600 [Abstract]
Tai, A. W., Chuang, J.-Z., Sung, C.-H. (1998). Localization of Tctex-1, a Cytoplasmic Dynein Light Chain, to the Golgi Apparatus and Evidence for Dynein Complex Heterogeneity. J. Biol. Chem. 273: 19639-19649 [Abstract] [Full Text]
Jesch, S. A., Linstedt, A. D. (1998). The Golgi and Endoplasmic Reticulum Remain Independent during Mitosis in HeLa Cells. Mol. Biol. Cell 9: 623-635 [Abstract] [Full Text]
Pryde, J. G., Farmaki, T., Lucocq, J. M. (1998). Okadaic Acid Induces Selective Arrest of Protein Transport in the Rough Endoplasmic Reticulum and Prevents Export into COPII-Coated Structures. Mol. Cell. Biol. 18: 1125-1135 [Abstract] [Full Text]
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Mironov, A., Colanzi, A., Silletta, M. G., Fiucci, G., Flati, S., Fusella, A., Polishchuk, R., Mironov, A. Jr., Tullio, G. D., Weigert, R., Malhotra, V., Corda, D., Matteis, M. A. D., Luini, A. (1997). Role of NAD+ and ADP-Ribosylation in the Maintenance of the Golgi Structure. JCB 139: 1109-1118 [Abstract] [Full Text]
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Taylor, R. S., Jones, S. M., Dahl, R. H., Nordeen, M. H., Howell, K. E. (1997). Characterization of the Golgi Complex Cleared of Proteins in Transit and Examination of Calcium Uptake Activities. Mol. Biol. Cell 8: 1911-1931 [Abstract] [Full Text]
Bannykh, S. I., Balch, W. E. (1997). Membrane Dynamics at the Endoplasmic Reticulum-Golgi Interface. JCB 138: 1-4 [Full Text]
Shima, D. T., Haldar, K., Pepperkok, R., Watson, R., Warren, G. (1997). Partitioning of the Golgi Apparatus during Mitosis in Living HeLa Cells. JCB 137: 1211-1228 [Abstract] [Full Text]
Wang, H., Benlimame, N, Nabi, I (1997). The AMF-R tubule is a smooth ilimaquinone-sensitive subdomain of the endoplasmic reticulum. J. Cell Sci. 110: 3043-3053 [Abstract]
Levine, T. P., Rabouille, C., Kieckbusch, R. H., Warren, G. (1996). Binding of the Vesicle Docking Protein p115 to Golgi Membranes Is Inhibited under Mitotic Conditions. J. Biol. Chem. 271: 17304-17311 [Abstract] [Full Text]
Simon, J.-P., Ivanov, I. E., Shopsin, B., Hersh, D., Adesnik, M., Sabatini, D. D. (1996). The in Vitro Generation of Post-Golgi Vesicles Carrying Viral Envelope Glycoproteins Requires an ARF-like GTP-binding Protein and a Protein Kinase C Associated with the Golgi Apparatus. J. Biol. Chem. 271: 16952-16961 [Abstract] [Full Text]
Nilsson, T, Rabouille, C, Hui, N, Watson, R, Warren, G (1996). The role of the membrane-spanning domain and stalk region of N-acetylglucosaminyltransferase I in retention, kin recognition and structural maintenance of the Golgi apparatus in HeLa cells. J. Cell Sci. 109: 1975-1989 [Abstract]
Misteli, T, Warren, G (1995). Mitotic disassembly of the Golgi apparatus in vivo. J. Cell Sci. 108: 2715-2727 [Abstract]
Rabouille, C, Hui, N, Hunte, F, Kieckbusch, R, Berger, E., Warren, G, Nilsson, T (1995). Mapping the distribution of Golgi enzymes involved in the construction of complex oligosaccharides. J. Cell Sci. 108: 1617-1627 [Abstract]
Bannykh, S., Aridor, M., Plutner, H., Rowe, T., Balch, W.E. (1995). Regulated Export of Cargo from the Endoplasmic Reticulum of Mammalian Cells. Cold Spring Harb Symp Quant Biol 60: 127-137 [Abstract]
Levine, T.P., Misteli, T., Rabouille, C., Warren, G. (1995). Mitotic Disassembly and Reassembly of the Golgi Apparatus. Cold Spring Harb Symp Quant Biol 60: 549-557 [Abstract]
Acharya, U., Malhotra, V. (1995). Reconstitution of Golgi Stacks from Vesiculated Golgi Membranes in Permeabilized Cells. Cold Spring Harb Symp Quant Biol 60: 559-566 [Abstract]
Marks, D., Larkin, J., McNiven, M. (1994). Association of kinesin with the Golgi apparatus in rat hepatocytes. J. Cell Sci. 107: 2417-2426 [Abstract]
Burdett, I. (1993). Internalisation of desmosomes and their entry into the endocytic pathway via late endosomes in MDCK cells. Possible mechanisms for the modulation of cell adhesion by desmosomes during development. J. Cell Sci. 106: 1115-1130 [Abstract]
Zhu, Q, Liu, T, Clarke, M (1993). Calmodulin and the contractile vacuole complex in mitotic cells of Dictyostelium discoideum. J. Cell Sci. 104: 1119-1127 [Abstract]
Pypaert, M, Nilsson, T, Berger, E., Warren, G (1993). Mitotic Golgi clusters are not tubular endosomes. J. Cell Sci. 104: 811-818 [Abstract]
Rudick, V., Rudick, M., Brun-Zinkernagel, A. (1993). Amplification of the Golgi complex in MDCK cells secreting human growth hormone. J. Cell Sci. 104: 509-520 [Abstract]
Lucocq, J. (1992). Mimicking mitotic Golgi disassembly using okadaic acid. J. Cell Sci. 103: 875-880
Thyberg, J., Moskalewski, S. (1992). Disorganization of the Golgi complex and the cytoplasmic microtubule system in CHO cells exposed to okadaic acid. J. Cell Sci. 103: 1167-1175 [Abstract]
Alvarez, C., Garcia-Mata, R., Hauri, H.-P., Sztul, E. (2001). The p115-interactive Proteins GM130 and Giantin Participate in Endoplasmic Reticulum-Golgi Traffic. J. Biol. Chem. 276: 2693-2700 [Abstract] [Full Text]
Puthenveedu, M. A., Linstedt, A. D. (2001). Evidence that Golgi structure depends on a p115 activity that is independent of the vesicle tether components giantin and GM130. JCB 155: 227-238 [Abstract] [Full Text]
Lane, J. D., Lucocq, J., Pryde, J., Barr, F. A., Woodman, P. G., Allan, V. J., Lowe, M. (2002). Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation during apoptosis. JCB 156: 495-509 [Abstract] [Full Text]