Basic fibroblast growth factor is an extracellular matrix component required for supporting the proliferation of vascular endothelial cells and the differentiation of PC12 cells

doi:10.1083/jcb.109.2.823

This Article

	Full Text (PDF, 3409K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Rogelj, S.
	Articles by Vlodavsky, I.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Rogelj, S.
	Articles by Vlodavsky, I.


Social Bookmarking

	What's this?

ARTICLES

Basic fibroblast growth factor is an extracellular matrix component required for supporting the proliferation of vascular endothelial cells and the differentiation of PC12 cells

S Rogelj, M Klagsbrun, R Atzmon, M Kurokawa, A Haimovitz, Z Fuks and I Vlodavsky
Department of Surgery, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115.

Vascular endothelial cells (ECs) seeded sparsely on extracellular matrix (ECM) will proliferate in the absence of exogenous basic fibroblast growth factor (bFGF). This ECM will also stimulate neurite outgrowth in PC12 cells in the absence of exogenous growth factors. We have previously shown that bFGF is found in subendothelial ECM (Vlodavsky, I., J. Folkman, R. Sullivan, R. Fridman, R. Ishai-Michaeli, J. Sasse, and M. Klagsburn. 1987. Proc. Natl. Acad. Sci. USA. 84:2292- 2296) and in basement membranes (Folkman, J., M. Klagsburn, J. Sasse, M. Wadzinski, D. Ingber, and I. Vlodavsky. 1988. Am. J. Pathol. 130:393- 400). The actual requirement of ECM-associated bFGF for the growth of ECs and differentiation of PC12 cells was shown in two ways. First, polyclonal anti-bFGF antibodies added to subendothelial ECM inhibited both EC proliferation and PC12 neurite outgrowth. Secondly, PF-HR-9 cells, which do not synthesize bFGF and which produce an ECM not permissive for EC proliferation and PC12 neurite outgrowth, were transfected with bFGF cDNA. PF-HR-9 cells transfected with bFGF, but not with the dominant selectable marker SV2-neomycin, were found to express bFGF and to produce an ECM which did support both EC proliferation and PC12 differentiation. The ECM-mediated stimulatory effects were inhibited by anti-bFGF antibodies but not by anti-nerve growth factor antibodies or nonimmune rabbit IgG. These results indicate that bFGF associated with ECM is a required ECM component for ECM-mediated cell proliferation and differentiation.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Zehe, C., Engling, A., Wegehingel, S., Schafer, T., Nickel, W. (2006). Cell-surface heparan sulfate proteoglycans are essential components of the unconventional export machinery of FGF-2. Proc. Natl. Acad. Sci. USA 103: 15479-15484 [Abstract] [Full Text]
Backhaus, R., Zehe, C., Wegehingel, S., Kehlenbach, A., Schwappach, B., Nickel, W. (2004). Unconventional protein secretion: membrane translocation of FGF-2 does not require protein unfolding. J. Cell Sci. 117: 1727-1736 [Abstract] [Full Text]
Schafer, T., Zentgraf, H., Zehe, C., Brugger, B., Bernhagen, J., Nickel, W. (2004). Unconventional Secretion of Fibroblast Growth Factor 2 Is Mediated by Direct Translocation across the Plasma Membrane of Mammalian Cells. J. Biol. Chem. 279: 6244-6251 [Abstract] [Full Text]
Margosio, B., Marchetti, D., Vergani, V., Giavazzi, R., Rusnati, M., Presta, M., Taraboletti, G. (2003). Thrombospondin 1 as a scavenger for matrix-associated fibroblast growth factor 2. Blood 102: 4399-4406 [Abstract] [Full Text]
Rieck, P. W., Gigon, M., Jaroszewski, J., Pleyer, U., Hartmann, C. (2003). Increased Endothelial Survival of Organ-Cultured Corneas Stored in FGF-2-Supplemented Serum-Free Medium. IOVS 44: 3826-3832 [Abstract] [Full Text]
Kagan, B. L., Henke, R. T., Cabal-Manzano, R., Stoica, G. E., Nguyen, Q., Wellstein, A., Riegel, A. T. (2003). Complex Regulation of the Fibroblast Growth Factor-binding Protein in MDA- MB-468 Breast Cancer Cells by CCAAT/Enhancer-binding Protein {beta}. Cancer Res. 63: 1696-1705 [Abstract] [Full Text]
Heilmann, C., von Samson, P., Schlegel, K., Attmann, T., von Specht, B.-U., Beyersdorf, F., Lutter, G. (2002). Comparison of protein with DNA therapy for chronic myocardial ischemia using fibroblast growth factor-2. Eur. J. Cardiothorac. Surg. 22: 957-964 [Abstract] [Full Text]
Dowd, C. J., Cooney, C. L., Nugent, M. A. (1999). Heparan Sulfate Mediates bFGF Transport through Basement Membrane by Diffusion with Rapid Reversible Binding. J. Biol. Chem. 274: 5236-5244 [Abstract] [Full Text]
Ribatti, D, Leali, D, Vacca, A, Giuliani, R, Gualandris, A, Roncali, L, Nolli, M., Presta, M (1999). In vivo angiogenic activity of urokinase: role of endogenous fibroblast growth factor-2. J. Cell Sci. 112: 4213-4221 [Abstract]
Hosokawa, K., Aharoni, D., Dantes, A., Shaulian, E., Schere-Levy, C., Atzmon, R., Kotsuji, F., Oren, M., Vlodavsky, I., Amsterdam, A. (1998). Modulation of Mdm2 Expression and p53-Induced Apoptosis in Immortalized Human Ovarian Granulosa Cells. Endocrinology 139: 4688-4700 [Abstract] [Full Text]
Seghezzi, G., Patel, S., Ren, C. J., Gualandris, A., Pintucci, G., Robbins, E. S., Shapiro, R. L., Galloway, A. C., Rifkin, D. B., Mignatti, P. (1998). Fibroblast Growth Factor-2 (FGF-2) Induces Vascular Endothelial Growth Factor (VEGF) Expression in the Endothelial Cells of Forming Capillaries: An Autocrine Mechanism Contributing to Angiogenesis. JCB 141: 1659-1673 [Abstract] [Full Text]
Florkiewicz, R. Z., Anchin, J., Baird, A. (1998). The Inhibition of Fibroblast Growth Factor-2 Export by Cardenolides Implies a Novel Function for the Catalytic Subunit of Na+,K+-ATPase. J. Biol. Chem. 273: 544-551 [Abstract] [Full Text]
Smith, G. M., Hale, J. H. (1997). Macrophage/Microglia Regulation of Astrocytic Tenascin: Synergistic Action of Transforming Growth Factor-beta and Basic Fibroblast Growth Factor. J. Neurosci. 17: 9624-9633 [Abstract] [Full Text]
Dinbergs, I. D., Brown, L., Edelman, E. R. (1996). Cellular Response to Transforming Growth Factor-beta 1 and Basic Fibroblast Growth Factor Depends on Release Kinetics and Extracellular Matrix Interactions. J. Biol. Chem. 271: 29822-29829 [Abstract] [Full Text]
Peifley, K. A., Alberts, G. F., Hsu, D. K.W., Feng, S.-L. Y., Winkles, J. A. (1996). Heparin-Binding Epidermal Growth Factor-Like Growth Factor Regulates Fibroblast Growth Factor-2 Expression in Aortic Smooth Muscle Cells. Circ. Res. 79: 263-270 [Abstract] [Full Text]
Sherman, L, Stocker, K., Morrison, R, Ciment, G (1993). Basic fibroblast growth factor (bFGF) acts intracellularly to cause the transdifferentiation of avian neural crest-derived Schwann cell precursors into melanocytes. Development 118: 1313-1326 [Abstract]
Brill, G, Vaisman, N, Neufeld, G, Kalcheim, C (1992). BHK-21-derived cell lines that produce basic fibroblast growth factor, but not parental BHK-21 cells, initiate neuronal differentiation of neural crest progenitors. Development 115: 1059-1069 [Abstract]