The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants

doi:10.1083/jcb.109.3.1015

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The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants

RA Bacon, A Salminen, H Ruohola, P Novick and S Ferro-Novick
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510.

The YPT1 gene encodes a raslike, GTP-binding protein that is essential for growth of yeast cells. We show here that mutations in the ypt1 gene disrupt transport of carboxypeptidase Y to the vacuole in vivo and transport of pro-alpha-factor to a site of extensive glycosylation in the Golgi apparatus in vitro. Two different ypt1 mutations result in loss of function of the Golgi complex without affecting the activity of the endoplasmic reticulum or soluble components required for in vitro transport. The function of the mutant Golgi apparatus can be restored by preincubation with wild-type cytosol. The transport defect observed in vitro cannot be overcome by addition of Ca++ to the reaction mixture. We have also established genetic interactions between ypt1 and a subset of the other genes required for transport to and through the Golgi apparatus.
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Barrowman, J., Wang, W., Zhang, Y., Ferro-Novick, S. (2003). The Yip1p{middle dot}Yif1p Complex Is Required for the Fusion Competence of Endoplasmic Reticulum-derived Vesicles. J. Biol. Chem. 278: 19878-19884 [Abstract] [Full Text]
Wang, W., Ferro-Novick, S. (2002). A Ypt32p Exchange Factor Is a Putative Effector of Ypt1p. Mol. Biol. Cell 13: 3336-3343 [Abstract] [Full Text]
Du, L.-L., Novick, P. (2001). Yeast Rab GTPase-activating Protein Gyp1p Localizes to the Golgi Apparatus and Is a Negative Regulator of Ypt1p. Mol. Biol. Cell 12: 1215-1226 [Abstract] [Full Text]
Jones, S., Newman, C., Liu, F., Segev, N. (2000). The TRAPP Complex Is a Nucleotide Exchanger for Ypt1 and Ypt31/32. Mol. Biol. Cell 11: 4403-4411 [Abstract] [Full Text]
Finger, F. P., Novick, P. (2000). Synthetic Interactions of the Post-Golgi sec Mutations of Saccharomyces cerevisiae. Genetics 156: 943-951 [Abstract] [Full Text]
Batoko, H., Zheng, H.-Q., Hawes, C., Moore, I. (2000). A Rab1 GTPase Is Required for Transport between the Endoplasmic Reticulum and Golgi Apparatus and for Normal Golgi Movement in Plants. Plant Cell 12: 2201-2218 [Abstract] [Full Text]
Wang, W., Sacher, M., Ferro-Novick, S. (2000). Trapp Stimulates Guanine Nucleotide Exchange on Ypt1p. JCB 151: 289-296 [Abstract] [Full Text]
Jones, S., Jedd, G., Kahn, R. A., Franzusoff, A., Bartolini, F., Segev, N. (1999). Genetic Interactions in Yeast Between Ypt GTPases and Arf Guanine Nucleotide Exchangers. Genetics 152: 1543-1556 [Abstract] [Full Text]
Richardson, C. J., Jones, S., Litt, R. J., Segev, N. (1998). GTP Hydrolysis Is Not Important for Ypt1 GTPase Function in Vesicular Transport. Mol. Cell. Biol. 18: 827-838 [Abstract] [Full Text]
Jedd, G., Mulholland, J., Segev, N. (1997). Two New Ypt GTPases Are Required for Exit From the Yeast trans-Golgi Compartment. JCB 137: 563-580 [Abstract] [Full Text]
Saraste, J, Lahtinen, U, Goud, B (1995). Localization of the small GTP-binding protein rab1p to early compartments of the secretory pathway. J. Cell Sci. 108: 1541-1552 [Abstract]
Ruby, S W, Chang, T H, Abelson, J (1993). Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA.. Genes Dev. 7: 1909-1925 [Abstract]
Deretic, D, Papermaster, D. (1993). Rab6 is associated with a compartment that transports rhodopsin from the trans-Golgi to the site of rod outer segment disk formation in frog retinal photoreceptors. J. Cell Sci. 106: 803-813 [Abstract]
Perou, C., Kaplan, J (1993). Chediak-Higashi syndrome is not due to a defect in microtubule-based lysosomal mobility. J. Cell Sci. 106: 99-107 [Abstract]
Tixier-Vidal, A, Barret, A, Picart, R, Mayau, V, Vogt, D, Wiedenmann, B, Goud, B (1993). The small GTP-binding protein, Rab6p, is associated with both Golgi and post-Golgi synaptophysin-containing membranes during synaptogenesis of hypothalamic neurons in culture. J. Cell Sci. 105: 935-947 [Abstract]
Antony, C., Cibert, C., Geraud, G., Santa Maria, A., Maro, B., Mayau, V., Goud, B. (1992). The small GTP-binding protein rab6p is distributed from medial Golgi to the trans-Golgi network as determined by a confocal microscopic approach. J. Cell Sci. 103: 785-796 [Abstract]
Morimoto, B H, Chuang, C C, Koshland, D E (1991). Molecular cloning of a member of a new class of low-molecular-weight GTP-binding proteins.. Genes Dev. 5: 2386-2391 [Abstract]
Segev, N (1991). Mediation of the attachment or fusion step in vesicular transport by the GTP-binding Ypt1 protein. Science 252: 1553-1556 [Abstract]
Hall, A (1990). The cellular functions of small GTP-binding proteins. Science 249: 635-640 [Abstract]
Ngsee, J.K., Trimble, W.S., Elferink, L.A., Wendland, B., Miller, K., Calakos, N., Scheller, R.H. (1990). Molecular Analysis of Proteins Associated with the Synaptic Vesicle Membrane. Cold Spring Harb Symp Quant Biol 55: 111-118 [Abstract]