The Journal of Cell Biology, Vol 109, 1023-1036, Copyright © 1989 by The Rockefeller University Press

ARTICLES

The Sec15 protein responds to the function of the GTP binding protein, Sec4, to control vesicular traffic in yeast

A Salminen and PJ Novick
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510.

SEC15 function is required at a late stage of the yeast secretory pathway. Duplication of the gene encoding the ras-like, GTP-binding protein, Sec4, can suppress the partial loss of function resulting from the sec15-l mutation, but cannot suppress disruption of sec15. Analysis of the SEC15 gene predicts a hydrophilic protein product of 105 kD. Anti-Sec15 antibody recognizes a protein of 116-kD apparent molecular mass which is associated with a microsomal fraction of yeast in a strongly pH dependent fashion. Overproduction of Sec15 protein interferes with the secretory pathway, resulting in the formation of a cluster of secretory vesicles, and a patch of Sec15 protein revealed by immunofluorescence. The sec4-8 and sec2-4l mutations, but not mutations in other SEC genes, prevent formation of the Sec15 protein patch. We propose that Sec15 protein responds to the function of the Sec4 protein to control vesicular traffic.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Kurischko, C., Kuravi, V. K., Wannissorn, N., Nazarov, P. A., Husain, M., Zhang, C., Shokat, K. M., McCaffery, J. M., Luca, F. C. (2008). The Yeast LATS/Ndr Kinase Cbk1 Regulates Growth via Golgi-dependent Glycosylation and Secretion. Mol. Biol. Cell 19: 5559-5578 [Abstract] [Full Text]  
• Giansanti, M. G., Belloni, G., Gatti, M. (2007). Rab11 Is Required for Membrane Trafficking and Actomyosin Ring Constriction in Meiotic Cytokinesis of Drosophila Males. Mol. Biol. Cell 18: 5034-5047 [Abstract] [Full Text]  
• De Craene, J.-O., Coleman, J., Estrada de Martin, P., Pypaert, M., Anderson, S., Yates, J. R. III, Ferro-Novick, S., Novick, P. (2006). Rtn1p Is Involved in Structuring the Cortical Endoplasmic Reticulum. Mol. Biol. Cell 17: 3009-3020 [Abstract] [Full Text]  
• Medkova, M., France, Y. E., Coleman, J., Novick, P. (2006). The rab Exchange Factor Sec2p Reversibly Associates with the Exocyst. Mol. Biol. Cell 17: 2757-2769 [Abstract] [Full Text]  
• France, Y. E., Boyd, C., Coleman, J., Novick, P. J. (2006). The polarity-establishment component Bem1p interacts with the exocyst complex through the Sec15p subunit. J. Cell Sci. 119: 876-888 [Abstract] [Full Text]  
• Zhang, Y., Higashide, W., Dai, S., Sherman, D. M., Zhou, D. (2005). Recognition and Ubiquitination of Salmonella Type III Effector SopA by a Ubiquitin E3 Ligase, HsRMA1. J. Biol. Chem. 280: 38682-38688 [Abstract] [Full Text]  
• Castillo-Flores, A., Weinberger, A., Robinson, M., Gerst, J. E. (2005). Mso1 Is a Novel Component of the Yeast Exocytic SNARE Complex. J. Biol. Chem. 280: 34033-34041 [Abstract] [Full Text]  
• Zajac, A., Sun, X., Zhang, J., Guo, W. (2005). Cyclical Regulation of the Exocyst and Cell Polarity Determinants for Polarized Cell Growth. Mol. Biol. Cell 16: 1500-1512 [Abstract] [Full Text]  
• Wiederkehr, A., De Craene, J.-O., Ferro-Novick, S., Novick, P. (2004). Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p. JCB 167: 875-887 [Abstract] [Full Text]  
• Zhang, X.-M., Ellis, S., Sriratana, A., Mitchell, C. A., Rowe, T. (2004). Sec15 Is an Effector for the Rab11 GTPase in Mammalian Cells. J. Biol. Chem. 279: 43027-43034 [Abstract] [Full Text]  
• Aronov, S., Gerst, J. E. (2004). Involvement of the Late Secretory Pathway in Actin Regulation and mRNA Transport in Yeast. J. Biol. Chem. 279: 36962-36971 [Abstract] [Full Text]  
• Toikkanen, J. H., Miller, K. J., Soderlund, H., Jantti, J., Keranen, S. (2003). The {beta} Subunit of the Sec61p Endoplasmic Reticulum Translocon Interacts with the Exocyst Complex in Saccharomyces cerevisiae. J. Biol. Chem. 278: 20946-20953 [Abstract] [Full Text]  
• Jantti, J., Aalto, M. K., Oyen, M., Sundqvist, L., Keranen, S., Ronne, H. (2002). Characterization of temperature-sensitive mutations in the yeast syntaxin 1 homologues Sso1p and Sso2p, and evidence of a distinct function for Sso1p in sporulation. J. Cell Sci. 115: 409-420 [Abstract] [Full Text]  
• Finger, F. P., Novick, P. (1998). Spatial Regulation of Exocytosis: Lessons from Yeast. JCB 142: 609-612 [Full Text]  
• Roth, D., Guo, W., Novick, P. (1998). Dominant Negative Alleles of SEC10 Reveal Distinct Domains Involved in Secretion and Morphogenesis in Yeast. Mol. Biol. Cell 9: 1725-1739 [Abstract] [Full Text]  
• Walch-Solimena, C., Collins, R. N., Novick, P. J. (1997). Sec2p Mediates Nucleotide Exchange on Sec4p and Is Involved in Polarized Delivery of Post-Golgi Vesicles. JCB 137: 1495-1509 [Abstract] [Full Text]  
• Sun, Z, Fay, D S, Marini, F, Foiani, M, Stern, D F (1996). Spk1/Rad53 is regulated by Mec1-dependent protein phosphorylation in DNA replication and damage checkpoint pathways.. Genes Dev. 10: 395-406 [Abstract]  
• Novick, P., Garrett, M.D., Brennwald, P., Lauring, A., Finger, F.P., Collins, R., TeeBush, D.R. (1995). Control of Exocytosis in Yeast. Cold Spring Harb Symp Quant Biol 60: 171-177 [Abstract]  
• Ruby, S W, Chang, T H, Abelson, J (1993). Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA.. Genes Dev. 7: 1909-1925 [Abstract]  
• Creutz, C. E., Kambouris, N. G., Snyder, S. L., Hamman, H. C., Nelson, M. R., Liu, W., Rock, P. (1992). Effects of the expression of mammalian annexins in yeast secretory mutants. J. Cell Sci. 103: 1177-1192 [Abstract]  
• Creutz, C. (1992). The annexins and exocytosis. Science 258: 924-931 [Abstract]  
• Hall, A (1990). The cellular functions of small GTP-binding proteins. Science 249: 635-640 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents