Evidence that calcium may control neurite outgrowth by regulating the stability of actin filaments

doi:10.1083/jcb.109.3.1229

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Evidence that calcium may control neurite outgrowth by regulating the stability of actin filaments

KL Lankford and PC Letourneau
Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis 55455.

We investigated the effects of calcium removal and calcium ionophores on the behavior and ultrastructure of cultured chick dorsal root ganglia (DRG) neurons to identify possible mechanisms by which calcium might regulate neurite outgrowth. Both calcium removal and the addition of calcium ionophores A23187 or ionomycin blocked outgrowth in previously elongating neurites, although in the case of calcium ionophores, changes in growth cone shape and retraction of neurites were also observed. Treatment with calcium ionophores significantly increased growth cone calcium. The ability of the microtubule stabilizing agent taxol to block A23187-induced neurite retraction and the ability of the actin stabilizing agent phalloidin to reverse both A23187-induced growth cone collapse and neurite retraction suggested that calcium acted on the cytoskeleton. Whole mount electron micrographs revealed an apparent disruption of actin filaments in the periphery (but not filopodia) of growth cones that were exposed to calcium ionophores in medium with normal calcium concentrations. This effect was not seen in cells treated with calcium ionophores in calcium- free medium or cells treated with the monovalent cation ionophore monensin, indicating that these effects were calcium specific. Ultrastructure of Triton X-100 extracted whole mounts further indicated that both microtubules and microfilaments may be more stable or extraction resistant after treatments which lower intracellular calcium. Taken together, the data suggest that calcium may control neurite elongation at least in part by regulating actin filament stability, and support a model for neurite outgrowth involving a balance between assembly and disassembly of the cytoskeleton.
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Lau, P.-m., Zucker, R. S., Bentley, D. (1999). Induction of Filopodia by Direct Local Elevation of Intracellular Calcium Ion Concentration. JCB 145: 1265-1276 [Abstract] [Full Text]
Steketee, M. B., Tosney, K. W. (1999). Contact with Isolated Sclerotome Cells Steers Sensory Growth Cones by Altering Distinct Elements of Extension. J. Neurosci. 19: 3495-3506 [Abstract] [Full Text]
Gallo, G., Letourneau, P. C. (1998). Localized Sources of Neurotrophins Initiate Axon Collateral Sprouting. J. Neurosci. 18: 5403-5414 [Abstract] [Full Text]
Horgan, A. M., Copenhaver, P. F. (1998). G Protein-Mediated Inhibition of Neuronal Migration Requires Calcium Influx. J. Neurosci. 18: 4189-4200 [Abstract] [Full Text]
Wu, G., Cline, H. T. (1998). Stabilization of Dendritic Arbor Structure in Vivo by CaMKII. Science 279: 222-226 [Abstract] [Full Text]
Furukawa, K., Fu, W., Li, Y., Witke, W., Kwiatkowski, D. J., Mattson, M. P. (1997). The Actin-Severing Protein Gelsolin Modulates Calcium Channel and NMDA Receptor Activities and Vulnerability to Excitotoxicity in Hippocampal Neurons. J. Neurosci. 17: 8178-8186 [Abstract] [Full Text]
Schindelholz, B., Reber, B. F.�X. (1997). Bradykinin-Induced Collapse of Rat Pheochromocytoma (PC12) Cell Growth Cones: A Role for Tyrosine Kinase Activity. J. Neurosci. 17: 8391-8401 [Abstract] [Full Text]
Lu, M., Witke, W., Kwiatkowski, D. J., Kosik, K. S. (1997). Delayed Retraction of Filopodia in Gelsolin Null Mice. JCB 138: 1279-1287 [Abstract] [Full Text]
Shorte, S. L. (1997). N-Methyl-D-Aspartate Evokes Rapid Net Depolymerization of Filamentous Actin in Cultured Rat Cerebellar Granule Cells. J. Neurophysiol. 78: 1135-1143 [Abstract] [Full Text]
Gallo, G., Lefcort, F. B., Letourneau, P. C. (1997). The trkA Receptor Mediates Growth Cone Turning toward a Localized Source of Nerve Growth Factor. J. Neurosci. 17: 5445-5454 [Abstract] [Full Text]
Bandtlow, C., Schmidt, M., Hassinger, T., Schwab, M., Kater, S. (1993). Role of intracellular calcium in NI-35-evoked collapse of neuronal growth cones. Science 259: 80-83 [Abstract]