Comparative studies of type X collagen expression in normal and rachitic chicken epiphyseal cartilage

doi:10.1083/jcb.109.4.1849

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Comparative studies of type X collagen expression in normal and rachitic chicken epiphyseal cartilage

AP Kwan, IR Dickson, AJ Freemont and ME Grant
Department of Biochemistry and Molecular Biology, University of Manchester, United Kingdom.

The levels of type X collagen in mineralizing normal chicken epiphyses and nonmineralizing rachitic chicken tibial epiphyses were measured and compared. Qualitative immunoperoxidase studies with anti-chick type X collagen monoclonal antibodies on sections from normal and rachitic cartilage demonstrated that the type X collagen levels in rachitic growth plates are reduced. Northern hybridization of mRNA and biosynthetic studies have confirmed that type X collagen synthesis in rickets is also decreased. In hypocalcemic rickets, the level of type X collagen mRNA is reduced by 80% whereas the level of type X collagen mRNA is only reduced by 50% in normocalcemic rickets. These observations provide additional evidence that type X collagen is involved in the process of cartilage mineralization and also suggest that the partial recovery of type X collagen synthesis in normocalcemic rickets may be related to the elevated plasma concentration of calcium. Calcium concentration may therefore play an important role in the control of type X collagen synthesis.
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