The notch gene product is a glycoprotein expressed on the cell surface of both epidermal and neuronal precursor cells during Drosophila development

doi:10.1083/jcb.109.5.2427

This Article

Full Text (PDF, 9226K)

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JCB

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Johansen, K. M.

Articles by Artavanis-Tsakonas, S.

Search for Related Content

PubMed

PubMed Citation

Articles by Johansen, K. M.

Articles by Artavanis-Tsakonas, S.

Pubmed/NCBI databases

Medline Plus Health Information
Gene	GEO Profiles
HomoloGene	UniGene
Compound via MeSH
Substance via MeSH
Genes and Gene Therapy

Social Bookmarking

What's this?

ARTICLES

The notch gene product is a glycoprotein expressed on the cell surface of both epidermal and neuronal precursor cells during Drosophila development

KM Johansen, RG Fehon and S Artavanis-Tsakonas
Department of Biology, Yale University, New Haven, Connecticut 06511.

The Notch locus of Drosophila melanogaster is one of a small number of zygotically acting "neurogenic" genes involved in the correct segregation of neural from epidermal lineages during embryogenesis as well as in other postembryonic developmental events. We have generated antibody probes against three regions of the Notch protein to study the expression of Notch and begin a biochemical characterization of the protein. Consistent with predictions based on DNA sequence data, here we gather evidence showing that Notch encodes a large, glycosylated surface protein with an apparent molecular mass of 300 kD: (a) all three antibodies detect Notch on Western blots as a high molecular mass, primarily full-length product; (b) immunoelectron microscopy localizes the Notch protein to the cell membrane; and (c) lentil lectin column binding demonstrates that the protein is glycosylated, indicative of its surface protein nature. In general, the distribution of the Notch protein coincides with that of the Notch transcript determined previously by in situ hybridizations. Notch is expressed in a much wider range of tissue types than those disrupted in the neurogenic mutant, as determined by antibody localization. Early labeling in the blastoderm appears ubiquitous except for the pole cells, but as development proceeds some distinctive features emerge: stronger staining is seen within the germ band layer where neuroblast delamination occurs, and the developing embryonic nervous system shows pronounced axonal staining. In third instar larvae, Notch is expressed in imaginal disks and in the central nervous system. Based on these results, certain models for how Notch controls the neuroblast cell fate choice are eliminated. We discuss how Notch may function in this choice as well as in other lineage fate determinations.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Bayraktar, J., Zygmunt, D., Carthew, R. W. (2006). Par-1 kinase establishes cell polarity and functions in Notch signaling in the Drosophila embryo. J. Cell Sci. 119: 711-721 [Abstract] [Full Text]
Cantera, R., Luer, K., Rusten, T. E., Barrio, R., Kafatos, F. C., Technau, G. M. (2003). Mutations in spalt cause a severe but reversible neurodegenerative phenotype in the embryonic central nervous system of Drosophila melanogaster. Development 129: 5577-5586 [Abstract] [Full Text]
Duan, H., Skeath, J. B., Nguyen, H. T. (2001). Drosophila Lame duck, a novel member of the Gli superfamily, acts as a key regulator of myogenesis by controlling fusion-competent myoblast development. Development 128: 4489-4500 [Abstract] [Full Text]
Wang, J., Shelly, L., Miele, L., Boykins, R., Norcross, M. A., Guan, E. (2001). Human Notch-1 Inhibits NF-{{kappa}}B Activity in the Nucleus Through a Direct Interaction Involving a Novel Domain. J. Immunol. 167: 289-295 [Abstract] [Full Text]
Hoemann, C. D., Beaulieu, N., Girard, L., Rebai, N., Jolicoeur, P. (2000). Two Distinct Notch1 Mutant Alleles Are Involved in the Induction of T-Cell Leukemia in c-myc Transgenic Mice. Mol. Cell. Biol. 20: 3831-3842 [Abstract] [Full Text]
Wesley, C. S., Saez, L. (2000). Analysis of Notch Lacking the Carboxyl Terminus Identified in Drosophila Embryos. JCB 149: 683-696 [Abstract] [Full Text]
Moloney, D. J., Shair, L. H., Lu, F. M., Xia, J., Locke, R., Matta, K. L., Haltiwanger, R. S. (2000). Mammalian Notch1 Is Modified with Two Unusual Forms of O-Linked Glycosylation Found on Epidermal Growth Factor-like Modules. J. Biol. Chem. 275: 9604-9611 [Abstract] [Full Text]
Klein, T, Arias, A. (1998). Interactions among Delta, Serrate and Fringe modulate Notch activity during Drosophila wing development. Development 125: 2951-2962 [Abstract]
Leshko-Lindsay, L., Corces, V. (1997). The role of selectins in Drosophila eye and bristle development. Development 124: 169-180 [Abstract]
Artavanis-Tsakonas, S, Matsuno, K, Fortini, M. (1995). Notch signaling. Science 268: 225-232 [Abstract]
Crittenden, S., Troemel, E., Evans, T., Kimble, J (1994). GLP-1 is localized to the mitotic region of the C. elegans germ line. Development 120: 2901-2911 [Abstract]
Lieber, T, Kidd, S, Alcamo, E, Corbin, V, Young, M W (1993). Antineurogenic phenotypes induced by truncated Notch proteins indicate a role in signal transduction and may point to a novel function for Notch in nuclei.. Genes Dev. 7: 1949-1965 [Abstract]
Giniger, E, Jan, L., Jan, Y. (1993). Specifying the path of the intersegmental nerve of the Drosophila embryo: a role for Delta and Notch. Development 117: 431-440 [Abstract]
Kooh, P., Fehon, R., Muskavitch, M. (1993). Implications of dynamic patterns of Delta and Notch expression for cellular interactions during Drosophila development. Development 117: 493-507 [Abstract]
Weinmaster, G., Roberts, V. J., Lemke, G. (1992). Notch2: a second mammalian Notch gene. Development 116: 931-941 [Abstract]
Xu, T, Caron, L., Fehon, R., Artavanis-Tsakonas, S (1992). The involvement of the Notch locus in Drosophila oogenesis. Development 115: 913-922 [Abstract]
Kimble, J., Crittenden, S., Lambie, E., Kodoyianni, V., Mango, S., Troemel, E. (1992). Regulation of Induction by GLP1, a Localized Cell Surface Receptor in Caenorhabditis elegans. Cold Spring Harb Symp Quant Biol 57: 401-407 [Abstract]
Baker, N., Mlodzik, M, Rubin, G. (1990). Spacing differentiation in the developing Drosophila eye: a fibrinogen-related lateral inhibitor encoded by scabrous. Science 250: 1370-1377 [Abstract]
Smoller, D, Friedel, C, Schmid, A, Bettler, D, Lam, L, Yedvobnick, B (1990). The Drosophila neurogenic locus mastermind encodes a nuclear protein unusually rich in amino acid homopolymers.. Genes Dev. 4: 1688-1700 [Abstract]
Coffman, C, Harris, W, Kintner, C (1990). Xotch, the Xenopus homolog of Drosophila notch. Science 249: 1438-1441 [Abstract]
Xu, T, Rebay, I, Fleming, R J, Scottgale, T N, Artavanis-Tsakonas, S (1990). The Notch locus and the genetic circuitry involved in early Drosophila neurogenesis.. Genes Dev. 4: 464-475 [Abstract]