A galactosyltransferase from the fission yeast Schizosaccharomyces pombe

doi:10.1083/jcb.109.6.2693

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ETHYLENEDIAMINE TETRAACETIC ACID
MANGANESE COMPOUNDS
MANGANESE, ELEMENTAL

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A galactosyltransferase from the fission yeast Schizosaccharomyces pombe

TG Chappell and G Warren
Imperial Cancer Research Fund, London, United Kingdom.

A membrane-associated galactosyltransferase has been purified to homogeneity from the fission yeast, Schizosaccharomyces pombe. The enzyme has a molecular weight of 61,000 and is capable of transfering galactose from UDP-galactose (UDP-Gal) to a variety of mannose-based acceptors to form an alpha-1,2 galactosyl mannoside linkage. Immunofluorescence localization of the protein is consistent with the presence of the enzyme in the Golgi apparatus of S. pombe. This, together with the presence of terminal, alpha-linked galactose on the N- linked oligosaccharides of S. pombe secretory proteins, suggests that the galactosyltransferase is an enzyme involved in the processing of glycoproteins transported through the Golgi apparatus in fission yeast.
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