The Journal of Cell Biology, Vol 109, 2929-2937, Copyright © 1989 by The Rockefeller University Press

ARTICLES

Cellular distribution of smooth muscle actins during mammalian embryogenesis: expression of the alpha-vascular but not the gamma- enteric isoform in differentiating striated myocytes

NM Sawtell and JL Lessard
Childrens Hospital Research Foundation, Basic Science Research, Cincinnati, Ohio 45229.

The cellular distribution of the alpha-vascular and gamma-enteric smooth muscle actin isoforms was analyzed in rat embryos from gestational day (gd) 8 through the first neonatal week by in situ antigen localization using isoactin specific monoclonal antibodies. The alpha-vascular actin isoform was first detected on gd 10 in discrete cells lining the embryonic vasculature. By gd 14, this isoform was also present in the inner layers of mesenchymal cells condensing around the developing airways and gut. The gamma-enteric actin, however, was not detected until gd 15 when cells surrounding the developing aorta, airways, and gut labeled with the gamma-enteric-specific probe. There was continued expression of these two actin isoforms in regions of developing smooth muscle through the remainder of gestation and first neonatal week at which time their distribution coincided with that found in the adult. In addition to developing smooth muscle, the alpha- vascular actin isoform was expressed in differentiating striated muscle cells. On gd 10, there was intense labeling with the alpha-vascular specific probe in developing myocardiocytes and, within 24 h, in somitic myotomal cells. Although significant levels of this smooth muscle actin were present in striated myocytes through gd 17, by the end of the first postnatal week, alpha-vascular actin was no longer detectable in either cardiac or skeletal muscle. Thus, the normal developmental sequence of striated muscle cells includes the transient expression of the alpha-vascular smooth muscle actin isoform. In contrast, the gamma-enteric smooth muscle actin was not detected at any time in embryonic striated muscle. The differential timing of appearance and distribution of these two smooth muscle isoforms indicates that their expression is independently regulated during development.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Sun, Q., Taurin, S., Sethakorn, N., Long, X., Imamura, M., Wang, D.-Z., Zimmer, W. E., Dulin, N. O., Miano, J. M. (2009). Myocardin-dependent Activation of the CArG Box-rich Smooth Muscle {gamma}-Actin Gene: PREFERENTIAL UTILIZATION OF A SINGLE CArG ELEMENT THROUGH FUNCTIONAL ASSOCIATION WITH THE NKX3.1 HOMEODOMAIN PROTEIN. J. Biol. Chem. 284: 32582-32590 [Abstract] [Full Text]  
• Gong, Z., Niklason, L. E. (2008). Small-diameter human vessel wall engineered from bone marrow-derived mesenchymal stem cells (hMSCs). FASEB J. 22: 1635-1648 [Abstract] [Full Text]  
• Weng, T., Chen, Z., Jin, N., Gao, L., Liu, L. (2006). Gene expression profiling identifies regulatory pathways involved in the late stage of rat fetal lung development. Am. J. Physiol. Lung Cell. Mol. Physiol. 291: L1027-L1037 [Abstract] [Full Text]  
• Chaqour, B., Yang, R., Sha, Q. (2006). Mechanical Stretch Modulates the Promoter Activity of the Profibrotic Factor CCN2 through Increased Actin Polymerization and NF-{kappa}B Activation. J. Biol. Chem. 281: 20608-20622 [Abstract] [Full Text]  
• Wicks, J, Haitchi, H M, Holgate, S T, Davies, D E, Powell, R M (2006). Enhanced upregulation of smooth muscle related transcripts by TGF{beta}2 in asthmatic (myo) fibroblasts. Thorax 61: 313-319 [Abstract] [Full Text]  
• Crawford, K., Flick, R., Close, L., Shelly, D., Paul, R., Bove, K., Kumar, A., Lessard, J. (2002). Mice Lacking Skeletal Muscle Actin Show Reduced Muscle Strength and Growth Deficits and Die during the Neonatal Period. Mol. Cell. Biol. 22: 5887-5896 [Abstract] [Full Text]  
• Beqaj, S., Jakkaraju, S., Mattingly, R. R., Pan, D., Schuger, L. (2002). High RhoA activity maintains the undifferentiated mesenchymal cell phenotype, whereas RhoA down-regulation by laminin-2 induces smooth muscle myogenesis. JCB 156: 893-903 [Abstract] [Full Text]  
• Gendron, R. L., Good, W. V., Adams, L. C., Paradis, H. (2001). Suppressed Expression of Tubedown-1 in Retinal Neovascularization of Proliferative Diabetic Retinopathy. IOVS 42: 3000-3007 [Abstract] [Full Text]  
• Halayko, A. J., Solway, J. (2001). Plasticity in Skeletal, Cardiac, and Smooth Muscle: Invited Review: Molecular mechanisms of phenotypic plasticity in smooth muscle cells. J. Appl. Physiol. 90: 358-368 [Abstract] [Full Text]  
• Herring, B. P., Lyons, G. E., Hoggatt, A. M., Gallagher, P. J. (2001). Telokin expression is restricted to smooth muscle tissues during mouse development. Am. J. Physiol. Cell Physiol. 280: C12-C21 [Abstract] [Full Text]  
• Graves, D. C., Yablonka–Reuveni, Z. (2000). Vascular Smooth Muscle Cells Spontaneously Adopt a Skeletal Muscle Phenotype: A Unique Myf5-/MyoD+ Myogenic Program. J. Histochem. Cytochem. 48: 1173-1194 [Abstract] [Full Text]  
• Thompson, R. L., Sawtell, N. M. (2000). Replication of Herpes Simplex Virus Type 1 within Trigeminal Ganglia Is Required for High Frequency but Not High Viral Genome Copy Number Latency. J. Virol. 74: 965-974 [Abstract] [Full Text]  
• Chen, B., Li, A., Wang, D., Wang, M., Zheng, L., Bartles, J. R. (1999). Espin Contains an Additional Actin-binding Site in Its N Terminus and Is a Major Actin-bundling Protein of the Sertoli Cell-Spermatid Ectoplasmic Specialization Junctional Plaque. Mol. Biol. Cell 10: 4327-4339 [Abstract] [Full Text]  
• Montanaro, F., Lindenbaum, M., Carbonetto, S. (1999). {alpha}-Dystroglycan Is a Laminin Receptor Involved in Extracellular Matrix Assembly on Myotubes and Muscle Cell Viability. JCB 145: 1325-1340 [Abstract] [Full Text]  
• Qian, J., Lorenz, J. N., Maeda, S., Sutliff, R. L., Weber, C., Nakayama, T., Colbert, M. C., Paul, R. J., Fagin, J. A., Clemens, T. L. (1999). Reduced Blood Pressure and Increased Sensitivity of the Vasculature to Parathyroid Hormone-Related Protein (PTHrP) in Transgenic Mice Overexpressing the PTH/PTHrP Receptor in Vascular Smooth Muscle. Endocrinology 140: 1826-1833 [Abstract] [Full Text]  
• Brault, V, Reedy, M., Sauder, U, Kammerer, R., Aebi, U, Schoenenberger, C (1999). Substitution of flight muscle-specific actin by human (beta)-cytoplasmic actin in the indirect flight muscle of Drosophila. J. Cell Sci. 112: 3627-3639 [Abstract]  
• Yang, Y, Relan, N., Przywara, D., Schuger, L (1999). Embryonic mesenchymal cells share the potential for smooth muscle differentiation: myogenesis is controlled by the cell's shape. Development 126: 3027-3033 [Abstract]  
• Sawtell, N. M., Poon, D. K., Tansky, C. S., Thompson, R. L. (1998). The Latent Herpes Simplex Virus Type 1 Genome Copy Number in Individual Neurons Is Virus Strain Specific and Correlates with Reactivation. J. Virol. 72: 5343-5350 [Abstract] [Full Text]  
• Wang, J., Niu, W., Witte, D. P., Chernausek, S. D., Nikiforov, Y. E., Clemens, T. L., Sharifi, B., Strauch, A. R., Fagin, J. A. (1998). Overexpression of Insulin-Like Growth Factor-Binding Protein-4 (IGFBP-4) in Smooth Muscle Cells of Transgenic Mice through a Smooth Muscle {alpha}-Actin-IGFBP-4 Fusion Gene Induces Smooth Muscle Hypoplasia. Endocrinology 139: 2605-2614 [Abstract] [Full Text]  
• Zilberman, A., Dave, V., Miano, J., Olson, E. N., Periasamy, M. (1998). Evolutionarily Conserved Promoter Region Containing CArG*-Like Elements Is Crucial for Smooth Muscle Myosin Heavy Chain Gene Expression. Circ. Res. 82: 566-575 [Abstract] [Full Text]  
• Yang, Y, Palmer, K., Relan, N, Diglio, C, Schuger, L (1998). Role of laminin polymerization at the epithelial mesenchymal interface in bronchial myogenesis. Development 125: 2621-2629 [Abstract]  
• Hungerford, J. E., Hoeffler, J. P., Bowers, C. W., Dahm, L. M., Falchetto, R., Shabanowitz, J., Hunt, D. F., Little, C. D. (1997). Identification of a Novel Marker for Primordial Smooth Muscle and Its Differential Expression Pattern in Contractile vs Noncontractile Cells. JCB 137: 925-937 [Abstract] [Full Text]  
• Kumar, A., Crawford, K., Close, L., Madison, M., Lorenz, J., Doetschman, T., Pawlowski, S., Duffy, J., Neumann, J., Robbins, J., Boivin, G. P., O'Toole, B. A., Lessard, J. L. (1997). Rescue of cardiac alpha -actin-deficient mice by enteric smooth muscle gamma -actin. Proc. Natl. Acad. Sci. USA 94: 4406-4411 [Abstract] [Full Text]  
• Samaha, F. F., Ip, H. S., Morrisey, E. E., Seltzer, J., Tang, Z., Solway, J., Parmacek, M. S. (1996). Developmental Pattern of Expression and Genomic Organization of the Calponin-h1 Gene. J. Biol. Chem. 271: 395-403 [Abstract] [Full Text]  
• Solway, J., Seltzer, J., Samaha, F. F., Kim, S., Alger, L. E., Niu, Q., Morrisey, E. E., Ip, H. S., Parmacek, M. S. (1995). Structure and Expression of a Smooth Muscle Cell-specific Gene, SM22[IMAGE]. J. Biol. Chem. 270: 13460-13469 [Abstract] [Full Text]  
• Saint-Jeannet, J., Levi, G, Girault, J., Koteliansky, V, Thiery, J. (1992). Ventrolateral regionalization of Xenopus laevis mesoderm is characterized by the expression of alpha-smooth muscle actin. Development 115: 1165-1173 [Abstract]  
• Beqaj, S., Jakkaraju, S., Mattingly, R. R., Pan, D., Schuger, L. (2002). High RhoA activity maintains the undifferentiated mesenchymal cell phenotype, whereas RhoA down-regulation by laminin-2 induces smooth muscle myogenesis. JCB 156: 893-903 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents