Mutations in the SAC1 gene suppress defects in yeast Golgi and yeast actin function

doi:10.1083/jcb.109.6.2939

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Mutations in the SAC1 gene suppress defects in yeast Golgi and yeast actin function

AE Cleves, PJ Novick and VA Bankaitis
Department of Microbiology, University of Illinois, Urbana-Champaign 61801.

The budding mode of Saccharomyces cerevisiae cell growth demands that a high degree of secretory polarity be established and directed toward the emerging bud. We report here our demonstration that mutations in SAC1, a gene identified by virtue of its allele-specific genetic interactions with yeast actin defects, were also capable of suppressing sec14 lethalities associated with yeast Golgi defects. Moreover, these sac1 suppressor properties also extended to sec6 and sec9 secretory vesicle defects. The genetic data are consistent with the notion that SAC1p modulates both secretory pathway and actin cytoskeleton function. On this basis, we suggest that SAC1p may represent one aspect of the mechanism whereby secretory and cytoskeletal activities are coordinated, so that proper spatial regulation of secretion might be achieved.
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Zhong, R., Burk, D. H., Nairn, C. J., Wood-Jones, A., Morrison, W. H. III, Ye, Z.-H. (2005). Mutation of SAC1, an Arabidopsis SAC Domain Phosphoinositide Phosphatase, Causes Alterations in Cell Morphogenesis, Cell Wall Synthesis, and Actin Organization. Plant Cell 17: 1449-1466 [Abstract] [Full Text]
Peterman, T. K., Ohol, Y. M., McReynolds, L. J., Luna, E. J. (2004). Patellin1, a Novel Sec14-Like Protein, Localizes to the Cell Plate and Binds Phosphoinositides. Plant Physiol. 136: 3080-3094 [Abstract] [Full Text]
Roth, M. G. (2004). Phosphoinositides in Constitutive Membrane Traffic. Physiol. Rev. 84: 699-730 [Abstract] [Full Text]
Coluccio, A., Malzone, M., Neiman, A. M. (2004). Genetic Evidence of a Role for Membrane Lipid Composition in the Regulation of Soluble NEM-Sensitive Factor Receptor Function in Saccharomyces cerevisiae. Genetics 166: 89-97 [Abstract] [Full Text]
Rohde, H. M., Cheong, F. Y., Konrad, G., Paiha, K., Mayinger, P., Boehmelt, G. (2003). The Human Phosphatidylinositol Phosphatase SAC1 Interacts with the Coatomer I Complex. J. Biol. Chem. 278: 52689-52699 [Abstract] [Full Text]
Zhong, R., Ye, Z.-H. (2003). The SAC Domain-Containing Protein Gene Family in Arabidopsis. Plant Physiol. 132: 544-555 [Abstract] [Full Text]
Howe, A. G., Zaremberg, V., McMaster, C. R. (2002). Cessation of Growth to Prevent Cell Death Due to Inhibition of Phosphatidylcholine Synthesis Is Impaired at 37 {degrees}C in Saccharomyces cerevisiae. J. Biol. Chem. 277: 44100-44107 [Abstract] [Full Text]
Yanagisawa, L. L., Marchena, J., Xie, Z., Li, X., Poon, P. P., Singer, R. A., Johnston, G. C., Randazzo, P. A., Bankaitis, V. A. (2002). Activity of Specific Lipid-regulated ADP Ribosylation Factor-GTPase-activating Proteins Is Required for Sec14p-dependent Golgi Secretory Function in Yeast. Mol. Biol. Cell 13: 2193-2206 [Abstract] [Full Text]
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Rudge, S. A., Zhou, C., Engebrecht, J. (2002). Differential Regulation of Saccharomyces cerevisiae Phospholipase D in Sporulation and Sec14-Independent Secretion. Genetics 160: 1353-1361 [Abstract] [Full Text]
Konrad, G., Schlecker, T., Faulhammer, F., Mayinger, P. (2002). Retention of the Yeast Sac1p Phosphatase in the Endoplasmic Reticulum Causes Distinct Changes in Cellular Phosphoinositide Levels and Stimulates Microsomal ATP Transport. J. Biol. Chem. 277: 10547-10554 [Abstract] [Full Text]
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Foti, M., Audhya, A., Emr, S. D. (2001). Sac1 Lipid Phosphatase and Stt4 Phosphatidylinositol 4-Kinase Regulate a Pool of Phosphatidylinositol 4-Phosphate That Functions in the Control of the Actin Cytoskeleton and Vacuole Morphology. Mol. Biol. Cell 12: 2396-2411 [Abstract] [Full Text]
Nakase, Y., Nakamura, T., Hirata, A., Routt, S. M., Skinner, H. B., Bankaitis, V. A., Shimoda, C. (2001). The Schizosaccharomyces pombe spo20+ Gene Encoding a Homologue of Saccharomyces cerevisiae Sec14 Plays an Important Role in Forespore Membrane Formation. Mol. Biol. Cell 12: 901-917 [Abstract] [Full Text]
Xie, Z., Fang, M., Bankaitis, V. A. (2001). Evidence for an Intrinsic Toxicity of Phosphatidylcholine to Sec14p-dependent Protein Transport from the Yeast Golgi Complex. Mol. Biol. Cell 12: 1117-1129 [Abstract] [Full Text]
Ooms, L. M., McColl, B. K., Wiradjaja, F., Wijayaratnam, A. P. W., Gleeson, P., Gething, M. J., Sambrook, J., Mitchell, C. A. (2000). The Yeast Inositol Polyphosphate 5-Phosphatases Inp52p and Inp53p Translocate to Actin Patches following Hyperosmotic Stress: Mechanism for Regulating Phosphatidylinositol 4,5-Bisphosphate at Plasma Membrane Invaginations. Mol. Cell. Biol. 20: 9376-9390 [Abstract] [Full Text]
Finger, F. P., Novick, P. (2000). Synthetic Interactions of the Post-Golgi sec Mutations of Saccharomyces cerevisiae. Genetics 156: 943-951 [Abstract] [Full Text]
Li, X., Routt, S. M., Xie, Z., Cui, X., Fang, M., Kearns, M. A., Bard, M., Kirsch, D. R., Bankaitis, V. A. (2000). Identification of a Novel Family of Nonclassic Yeast Phosphatidylinositol Transfer Proteins Whose Function Modulates Phospholipase D Activity and Sec14p-independent Cell Growth. Mol. Biol. Cell 11: 1989-2005 [Abstract] [Full Text]
Hughes, W. E., Woscholski, R., Cooke, F. T., Patrick, R. S., Dove, S. K., McDonald, N. Q., Parker, P. J. (2000). SAC1 Encodes a Regulated Lipid Phosphoinositide Phosphatase, Defects in Which Can Be Suppressed by the Homologous Inp52p and Inp53p Phosphatases. J. Biol. Chem. 275: 801-808 [Abstract] [Full Text]
Bensen, E. S., Costaguta, G., Payne, G. S. (2000). Synthetic Genetic Interactions With Temperature-Sensitive Clathrin in Saccharomyces cerevisiae: Roles for Synaptojanin-Like Inp53p and Dynamin-Related Vps1p in Clathrin-Dependent Protein Sorting at the trans-Golgi Network. Genetics 154: 83-97 [Abstract] [Full Text]
Spelbrink, R. G., Nothwehr, S. F. (1999). The Yeast GRD20 Gene Is Required for Protein Sorting in the trans-Golgi Network/Endosomal System and for Polarization of the Actin Cytoskeleton. Mol. Biol. Cell 10: 4263-4281 [Abstract] [Full Text]
Hama, H., Schnieders, E. A., Thorner, J., Takemoto, J. Y., DeWald, D. B. (1999). Direct Involvement of Phosphatidylinositol 4-Phosphate in Secretion in the Yeast Saccharomyces cerevisiae. J. Biol. Chem. 274: 34294-34300 [Abstract] [Full Text]
Rivas, M. P., Kearns, B. G., Xie, Z., Guo, S., Sekar, M. C., Hosaka, K., Kagiwada, S., York, J. D., Bankaitis, V. A. (1999). Pleiotropic Alterations in Lipid Metabolism in Yeast sac1 Mutants: Relationship to "Bypass Sec14p" and Inositol Auxotrophy. Mol. Biol. Cell 10: 2235-2250 [Abstract] [Full Text]
Stock, S. D., Hama, H., DeWald, D. B., Takemoto, J. Y. (1999). SEC14-dependent Secretion in Saccharomyces cerevisiae. NONDEPENDENCE ON SPHINGOLIPID SYNTHESIS-COUPLED DIACYLGLYCEROL PRODUCTION. J. Biol. Chem. 274: 12979-12983 [Abstract] [Full Text]
Guo, S., Stolz, L. E., Lemrow, S. M., York, J. D. (1999). SAC1-like Domains of Yeast SAC1, INP52, and INP53 and of Human Synaptojanin Encode Polyphosphoinositide Phosphatases. J. Biol. Chem. 274: 12990-12995 [Abstract] [Full Text]
Xie, Z., Fang, M., Rivas, M. P., Faulkner, A. J., Sternweis, P. C., Engebrecht, J., Bankaitis, V. A. (1998). Phospholipase D activity is required for suppression of yeast phosphatidylinositol transfer protein defects. Proc. Natl. Acad. Sci. USA 95: 12346-12351 [Abstract] [Full Text]
Stolz, L. E., Kuo, W. J., Longchamps, J., Sekhon, M. K., York, J. D. (1998). INP51, a Yeast Inositol Polyphosphate 5-Phosphatase Required for Phosphatidylinositol 4,5-Bisphosphate Homeostasis and Whose Absence Confers a Cold-resistant Phenotype. J. Biol. Chem. 273: 11852-11861 [Abstract] [Full Text]
Stolz, L. E., Huynh, C. V., Thorner, J., York, J. D. (1998). Identification and Characterization of an Essential Family of Inositol Polyphosphate 5-Phosphatases (INP51, INP52 and INP53 Gene Products) in the Yeast Saccharomyces cerevisiae. Genetics 148: 1715-1729 [Abstract] [Full Text]
Erdman, S., Lin, L., Malczynski, M., Snyder, M. (1998). Pheromone-regulated Genes Required for Yeast Mating Differentiation. JCB 140: 461-483 [Abstract] [Full Text]
Singer-Kruger, B, Nemoto, Y, Daniell, L, Ferro-Novick, S, De Camilli, P (1998). Synaptojanin family members are implicated in endocytic membrane traffic in yeast. J. Cell Sci. 111: 3347-3356 [Abstract]
Nemoto, Y., Arribas, M., Haffner, C., DeCamilli, P. (1997). Synaptojanin 2,�a Novel Synaptojanin Isoform with a Distinct Targeting Domain and Expression Pattern. J. Biol. Chem. 272: 30817-30821 [Abstract] [Full Text]
Chung, J.-K., Sekiya, F., Kang, H.-S., Lee, C., Han, J.-S., Kim, S. R., Bae, Y. S., Morris, A. J., Rhee, S. G. (1997). Synaptojanin Inhibition of Phospholipase D Activity by Hydrolysis of Phosphatidylinositol 4,5-Bisphosphate. J. Biol. Chem. 272: 15980-15985 [Abstract] [Full Text]
de Heuvel, E., Bell, A. W., Ramjaun, A. R., Wong, K., Sossin, W. S., McPherson, P. S. (1997). Identification of the Major Synaptojanin-binding Proteins in Brain. J. Biol. Chem. 272: 8710-8716 [Abstract] [Full Text]
Bajjalieh, S. M., Scheller, R. H. (1995). The Biochemistry of Neurotransmitter Secretion. J. Biol. Chem. 270: 1971-1974 [Full Text]
Nemoto, Y., Kearns, B. G., Wenk, M. R., Chen, H., Mori, K., Alb, J. G. Jr., De Camilli, P., Bankaitis, V. A. (2000). Functional Characterization of a Mammalian Sac1 and Mutants Exhibiting Substrate-specific Defects in Phosphoinositide Phosphatase Activity. J. Biol. Chem. 275: 34293-34305 [Abstract] [Full Text]
Xu, Y., Liu, Y., Ridgway, N. D., McMaster, C. R. (2001). Novel Members of the Human Oxysterol-binding Protein Family Bind Phospholipids and Regulate Vesicle Transport. J. Biol. Chem. 276: 18407-18414 [Abstract] [Full Text]
Minagawa, T., Ijuin, T., Mochizuki, Y., Takenawa, T. (2001). Identification and Characterization of a Sac Domain-containing Phosphoinositide 5-Phosphatase. J. Biol. Chem. 276: 22011-22015 [Abstract] [Full Text]
Nemoto, Y., Wenk, M. R., Watanabe, M., Daniell, L., Murakami, T., Ringstad, N., Yamada, H., Takei, K., De Camilli, P. (2001). Identification and Characterization of a Synaptojanin 2 Splice Isoform Predominantly Expressed in Nerve Terminals. J. Biol. Chem. 276: 41133-41142 [Abstract] [Full Text]
Li, X., Rivas, M. P., Fang, M., Marchena, J., Mehrotra, B., Chaudhary, A., Feng, L., Prestwich, G. D., Bankaitis, V. A. (2002). Analysis of oxysterol binding protein homologue Kes1p function in regulation of Sec14p-dependent protein transport from the yeast Golgi complex. JCB 157: 63-78 [Abstract] [Full Text]