Actin-binding proteins from Drosophila embryos: a complex network of interacting proteins detected by F-actin affinity chromatography

doi:10.1083/jcb.109.6.2963

This Article

	Full Text (PDF, 4967K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Miller, K. G.
	Articles by Alberts, B. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Miller, K. G.
	Articles by Alberts, B. M.


Social Bookmarking

	What's this?

ARTICLES

Actin-binding proteins from Drosophila embryos: a complex network of interacting proteins detected by F-actin affinity chromatography

KG Miller, CM Field and BM Alberts
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.

By using F-actin affinity chromatography columns to select proteins solely by their ability to bind to actin filaments, we have identified and partially purified greater than 40 proteins from early Drosophila embryos. These proteins represent approximately 0.5% of the total protein present in soluble cell extracts, and 2 mg are obtained by chromatography of an extract from 10 g of embryos. As judged by immunofluorescence of fixed embryos, 90% of the proteins that we have detected in F-actin column eluates are actin-associated in vivo (12 of 13 proteins tested). The distributions of antigens observed suggest that groups of these proteins cooperate in generating unique actin structures at different places in the cell. These structures change as cells progress through the cell cycle and as they undergo the specializations that accompany development. The variety of different spatial localizations that we have observed in a small subset of the total actin-binding proteins suggests that the actin cytoskeleton is a very complex network of interacting proteins.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Paolo D'Avino, P. (2009). How to scaffold the contractile ring for a safe cytokinesis - lessons from Anillin-related proteins. J. Cell Sci. 122: 1071-1079 [Abstract] [Full Text]
Noguchi, T., Frank, D. J., Isaji, M., Miller, K. G. (2009). Coiled-Coil-Mediated Dimerization Is Not Required for Myosin VI to Stabilize Actin during Spermatid Individualization in Drosophila melanogaster. Mol. Biol. Cell 20: 358-367 [Abstract] [Full Text]
Wu, V. M., Yu, M. H., Paik, R., Banerjee, S., Liang, Z., Paul, S. M., Bhat, M. A., Beitel, G. J. (2007). Drosophila Varicose, a member of a new subgroup of basolateral MAGUKs, is required for septate junctions and tracheal morphogenesis. Development 134: 999-1009 [Abstract] [Full Text]
Le, T., Liang, Z., Patel, H., Yu, M. H., Sivasubramaniam, G., Slovitt, M., Tanentzapf, G., Mohanty, N., Paul, S. M., Wu, V. M., Beitel, G. J. (2006). A New Family of Drosophila Balancer Chromosomes With a w- dfd-GMR Yellow Fluorescent Protein Marker. Genetics 174: 2255-2257 [Abstract] [Full Text]
Frank, D. J., Martin, S. R., Gruender, B. N. T., Lee, Y.-S. R., Simonette, R. A., Bayley, P. M., Miller, K. G., Beckingham, K. M. (2006). Androcam Is a Tissue-specific Light Chain for Myosin VI in the Drosophila Testis. J. Biol. Chem. 281: 24728-24736 [Abstract] [Full Text]
Frescas, D., Mavrakis, M., Lorenz, H., DeLotto, R., Lippincott-Schwartz, J. (2006). The secretory membrane system in the Drosophila syncytial blastoderm embryo exists as functionally compartmentalized units around individual nuclei. JCB 173: 219-230 [Abstract] [Full Text]
Hall, P. A., Todd, C. B., Hyland, P. L., McDade, S. S., Grabsch, H., Dattani, M., Hillan, K. J., Russell, S.E. H. (2005). The Septin-Binding Protein Anillin Is Overexpressed in Diverse Human Tumors. Clin. Cancer Res. 11: 6780-6786 [Abstract] [Full Text]
Royou, A., Field, C., Sisson, J. C., Sullivan, W., Karess, R. (2004). Reassessing the Role and Dynamics of Nonmuscle Myosin II during Furrow Formation in Early Drosophila Embryos. Mol. Biol. Cell 15: 838-850 [Abstract] [Full Text]
Wu, V. M., Schulte, J., Hirschi, A., Tepass, U., Beitel, G. J. (2004). Sinuous is a Drosophila claudin required for septate junction organization and epithelial tube size control. JCB 164: 313-323 [Abstract] [Full Text]
Clark, K. A., McGrail, M., Beckerle, M. C. (2003). Analysis of PINCH function in Drosophila demonstrates its requirement in integrin-dependent cellular processes. Development 130: 2611-2621 [Abstract] [Full Text]
Dumstrei, K., Wang, F., Shy, D., Tepass, U., Hartenstein, V. (2002). Interaction between EGFR signaling and DE-cadherin during nervous system morphogenesis. Development 129: 3983-3994 [Abstract] [Full Text]
Sisson, J. C., Field, C., Ventura, R., Royou, A., Sullivan, W. (2000). Lava Lamp, a Novel Peripheral Golgi Protein, Is Required for Drosophila melanogaster Cellularization. JCB 151: 905-918 [Abstract] [Full Text]
Adam, J. C., Pringle, J. R., Peifer, M. (2000). Evidence for Functional Differentiation among Drosophila Septins in Cytokinesis and Cellularization. Mol. Biol. Cell 11: 3123-3135 [Abstract] [Full Text]
Inoue, Y. H., do Carmo Avides, M., Shiraki, M., Deak, P., Yamaguchi, M., Nishimoto, Y., Matsukage, A., Glover, D. M. (2000). Orbit, a Novel Microtubule-Associated Protein Essential for Mitosis in Drosophila melanogaster. JCB 149: 153-166 [Abstract] [Full Text]
Foe, V., Field, C., Odell, G. (2000). Microtubules and mitotic cycle phase modulate spatiotemporal distributions of F-actin and myosin II in Drosophila syncytial blastoderm embryos. Development 127: 1767-1787 [Abstract]
Li, M.-g., Serr, M., Edwards, K., Ludmann, S., Yamamoto, D., Tilney, L. G., Field, C. M., Hays, T. S. (1999). Filamin Is Required for Ring Canal Assembly and Actin Organization during Drosophila Oogenesis. JCB 146: 1061-1074 [Abstract] [Full Text]
Rothwell, W., Zhang, C., Zelano, C, Hsieh, T., Sullivan, W (1999). The Drosophila centrosomal protein Nuf is required for recruiting Dah, a membrane associated protein, to furrows in the early embryo. J. Cell Sci. 112: 2885-2893 [Abstract]
Giansanti, M., Bonaccorsi, S, Gatti, M (1999). The role of anillin in meiotic cytokinesis of Drosophila males. J. Cell Sci. 112: 2323-2334 [Abstract]
Lekven, A., Tepass, U, Keshmeshian, M, Hartenstein, V (1998). faint sausage encodes a novel extracellular protein of the immunoglobulin superfamily required for cell migration and the establishment of normal axonal pathways in the Drosophila nervous system. Development 125: 2747-2758 [Abstract]
Rothwell, W., Fogarty, P, Field, C., Sullivan, W (1998). Nuclear-fallout, a Drosophila protein that cycles from the cytoplasm to the centrosomes, regulates cortical microfilament organization. Development 125: 1295-1303 [Abstract]
Ho, K, Dunin-Borkowski, O., Akam, M (1997). Cellularization in locust embryos occurs before blastoderm formation. Development 124: 2761-2768 [Abstract]
Tepass, U, Gruszynski-DeFeo, E, Haag, T A, Omatyar, L, Torok, T, Hartenstein, V (1996). shotgun encodes Drosophila E-cadherin and is preferentially required during cell rearrangement in the neurectoderm and other morphogenetically active epithelia.. Genes Dev. 10: 672-685 [Abstract]
Hime, G., Brill, J., Fuller, M. (1996). Assembly of ring canals in the male germ line from structural components of the contractile ring. J. Cell Sci. 109: 2779-2788 [Abstract]
Straub, K., Stella, M., Leptin, M (1996). The gelsolin-related flightless I protein is required for actin distribution during cellularisation in Drosophila. J. Cell Sci. 109: 263-270 [Abstract]
Peifer, M, Sweeton, D, Casey, M, Wieschaus, E (1994). wingless signal and Zeste-white 3 kinase trigger opposing changes in the intracellular distribution of Armadillo. Development 120: 369-380 [Abstract]
Peifer, M (1993). The product of the Drosophila segment polarity gene armadillo is part of a multi-protein complex resembling the vertebrate adherens junction. J. Cell Sci. 105: 993-1000 [Abstract]
Peifer, M, Orsulic, S, Sweeton, D, Wieschaus, E (1993). A role for the Drosophila segment polarity gene armadillo in cell adhesion and cytoskeletal integrity during oogenesis. Development 118: 1191-1207 [Abstract]
Sullivan, W, Fogarty, P, Theurkauf, W (1993). Mutations affecting the cytoskeletal organization of syncytial Drosophila embryos. Development 118: 1245-1254 [Abstract]
Callaini, G, Dallai, R, Riparbelli, M. (1993). F-actin domains in the syncytial blastoderm of the dipteran Ceratitis capitata. J. Cell Sci. 104: 97-104 [Abstract]
Rose, L S, Wieschaus, E (1992). The Drosophila cellularization gene nullo produces a blastoderm-specific transcript whose levels respond to the nucleocytoplasmic ratio.. Genes Dev. 6: 1255-1268 [Abstract]
Schweisguth, F, Lepesant, J A, Vincent, A (1990). The serendipity alpha gene encodes a membrane-associated protein required for the cellularization of the Drosophila embryo.. Genes Dev. 4: 922-931 [Abstract]