Microvesicles of the neurohypophysis are biochemically related to small synaptic vesicles of presynaptic nerve terminals

doi:10.1083/jcb.109.6.3425

This Article

	Full Text (PDF, 3848K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Navone, F.
	Articles by De Camilli, P.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Navone, F.
	Articles by De Camilli, P.


Social Bookmarking

	What's this?

ARTICLES

Microvesicles of the neurohypophysis are biochemically related to small synaptic vesicles of presynaptic nerve terminals

F Navone, G Di Gioia, R Jahn, M Browning, P Greengard and P De Camilli
Consiglio Nazionale delle Ricerche Center of Cytopharmacology, University of Milano, Italy.

Nerve endings of the posterior pituitary are densely populated by dense- core neurosecretory granules which are the storage sites for peptide neurohormones. In addition, they contain numerous clear microvesicles which are the same size as small synaptic vesicles of typical presynaptic nerve terminals. Several of the major proteins of small synaptic vesicles of presynaptic nerve terminals are present at high concentration in the posterior pituitary. We have now investigated the subcellular localization of such proteins. By immunogold electron microscopy carried out on bovine neurohypophysis we have found that three of these proteins, synapsin I, Protein III, and synaptophysin (protein p38) were concentrated on microvesicles but were not detectable in the membranes of neurosecretory granules. In addition, we have studied the distribution of the same proteins and of the synaptic vesicle protein p65 in subcellular fractions of bovine posterior pituitaries obtained by sucrose density centrifugation. We have found that the intrinsic membrane proteins synaptophysin and p65 had an identical distribution and were restricted to low density fractions of the gradient which contained numerous clear microvesicles with a size range the same as that of small synaptic vesicles. The peripheral membrane proteins synapsin I and Protein III exhibited a broader distribution extending into the denser part of the gradient. However, the amount of these proteins clearly declined in the fractions preceding the peak of neurosecretory granules. Our results suggest that microvesicles of the neurohypophysis are biochemically related to small synaptic vesicles of all other nerve terminals and argue against the hypothesis that such vesicles represent an endocytic byproduct of exocytosis of neurosecretory granules.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Ledoux, V. A., Smejkalova, T., May, R. M., Cooke, B. M., Woolley, C. S. (2009). Estradiol Facilitates the Release of Neuropeptide Y to Suppress Hippocampus-Dependent Seizures. J. Neurosci. 29: 1457-1468 [Abstract] [Full Text]
Callewaere, C., Fernette, B., Raison, D., Mechighel, P., Burlet, A., Calas, A., Kitabgi, P., Parsadaniantz, S. M., Rostene, W. (2008). Cellular and Subcellular Evidence for Neuronal Interaction between the Chemokine Stromal Cell-Derived Factor-1/CXCL 12 and Vasopressin: Regulation in the Hypothalamo-Neurohypophysial System of the Brattleboro Rats. Endocrinology 149: 310-319 [Abstract] [Full Text]
Calegari, F., Coco, S., Taverna, E., Bassetti, M., Verderio, C., Corradi, N., Matteoli, M., Rosa, P. (1999). A Regulated Secretory Pathway in Cultured Hippocampal Astrocytes. J. Biol. Chem. 274: 22539-22547 [Abstract] [Full Text]
Matsumoto, K., Ebihara, K., Yamamoto, H., Tabuchi, H., Fukunaga, K., Yasunami, M., Ohkubo, H., Shichiri, M., Miyamoto, E. (1999). Cloning from Insulinoma Cells of Synapsin I Associated with Insulin Secretory Granules. J. Biol. Chem. 274: 2053-2059 [Abstract] [Full Text]
Benson, D. L., Tanaka, H. (1998). N-Cadherin Redistribution during Synaptogenesis in Hippocampal Neurons. J. Neurosci. 18: 6892-6904 [Abstract] [Full Text]
Pierre, K., Rougon, G., Allard, M., Bonhomme, R., Gennarini, G., Poulain, D. A., Theodosis, Dionysia. T. (1998). Regulated Expression of the Cell Adhesion Glycoprotein F3 in Adult Hypothalamic Magnocellular Neurons. J. Neurosci. 18: 5333-5343 [Abstract] [Full Text]
Jeng, C.-J., McCarroll, S. A., Martin, T. F. J., Floor, E., Adams, J., Krantz, D., Butz, S., Edwards, R., Schweitzer, E. S. (1998). Thy-1 Is a Component Common to Multiple Populations of Synaptic Vesicles. JCB 140: 685-698 [Abstract] [Full Text]
Pupier, S., Leveque, C., Marqueze, B., Kataoka, M., Takahashi, M., Seagar, M. J. (1997). Cysteine String Proteins Associated with Secretory Granules of the Rat Neurohypophysis. J. Neurosci. 17: 2722-2727 [Abstract] [Full Text]
Nayak, A.�S., Moore, C.�I., Browning, M.�D. (1996). Ca2+/calmodulin-dependent protein kinase II phosphorylation of the presynaptic protein synapsin I is persistently increased during long-term�potentiation. Proc. Natl. Acad. Sci. USA 93: 15451-15456 [Abstract] [Full Text]
Baurfend, R., David, C., Galli, T., McPherson, P.S., Takei, K., De Camilli, P. (1995). Molecular Mechanisms in Synaptic Vesicle Endocytosis. Cold Spring Harb Symp Quant Biol 60: 397-404 [Abstract]
Oyarce, A., Eipper, B. (1995). Identification of subcellular compartments containing peptidylglycine alpha-amidating monooxygenase in rat anterior pituitary. J. Cell Sci. 108: 287-297 [Abstract]