Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells

doi:10.1083/jcb.110.1.105

This Article

Full Text (PDF, 2209K)

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JCB

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Haarer, B. K.

Articles by Brown, S. S.

Search for Related Content

PubMed

PubMed Citation

Articles by Haarer, B. K.

Articles by Brown, S. S.

Pubmed/NCBI databases

Gene	GEO Profiles
HomoloGene
Substance via MeSH

Social Bookmarking

What's this?

ARTICLES

Purification of profilin from Saccharomyces cerevisiae and analysis of profilin-deficient cells

BK Haarer, SH Lillie, AE Adams, V Magdolen, W Bandlow and SS Brown
Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor 48109.

We have isolated profilin from yeast (Saccharomyces cerevisiae) and have microsequenced a portion of the protein to confirm its identity; the region microsequenced agrees with the predicted amino acid sequence from a profilin gene recently isolated from S. cerevisiae (Magdolen, V., U. Oechsner, G. Muller, and W. Bandlow. 1988. Mol. Cell. Biol. 8:5108-5115). Yeast profilin resembles profilins from other organisms in molecular mass and in the ability to bind to polyproline, retard the rate of actin polymerization, and inhibit hydrolysis of ATP by monomeric actin. Using strains that carry disruptions or deletions of the profilin gene, we have found that, under appropriate conditions, cells can survive without detectable profilin. Such cells grow slowly, are temperature sensitive, lose the normal ellipsoidal shape of yeast cells, often become multinucleate, and generally grow much larger than wild-type cells. In addition, these cells exhibit delocalized deposition of cell wall chitin and have dramatically altered actin distributions.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Vidali, L., Augustine, R. C., Kleinman, K. P., Bezanilla, M. (2007). Profilin Is Essential for Tip Growth in the Moss Physcomitrella patens. Plant Cell 19: 3705-3722 [Abstract] [Full Text]
Buttery, S. M., Yoshida, S., Pellman, D. (2007). Yeast Formins Bni1 and Bnr1 Utilize Different Modes of Cortical Interaction during the Assembly of Actin Cables. Mol. Biol. Cell 18: 1826-1838 [Abstract] [Full Text]
Park, H.-O., Bi, E. (2007). Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond. Microbiol. Mol. Biol. Rev. 71: 48-96 [Abstract] [Full Text]
Moseley, J. B., Goode, B. L. (2006). The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism. Microbiol. Mol. Biol. Rev. 70: 605-645 [Abstract] [Full Text]
Rawe, V. Y., Payne, C., Schatten, G. (2006). Profilin and actin-related proteins regulate microfilament dynamics during early mammalian embryogenesis. Hum Reprod 21: 1143-1153 [Abstract] [Full Text]
Clark, M. G., Teply, J., Haarer, B. K., Viggiano, S. C., Sept, D., Amberg, D. C. (2006). A Genetic Dissection of Aip1p's Interactions Leads to a Model for Aip1p-Cofilin Cooperative Activities. Mol. Biol. Cell 17: 1971-1984 [Abstract] [Full Text]
Obermann, H., Raabe, I., Balvers, M., Brunswig, B., Schulze, W., Kirchhoff, C. (2005). Novel testis-expressed profilin IV associated with acrosome biogenesis and spermatid elongation. Mol Hum Reprod 11: 53-64 [Abstract] [Full Text]
Grimme, S. J., Gao, X.-D., Martin, P. S., Tu, K., Tcheperegine, S. E., Corrado, K., Farewell, A. E., Orlean, P., Bi, E. (2004). Deficiencies in the Endoplasmic Reticulum (ER)-Membrane Protein Gab1p Perturb Transfer of Glycosylphosphatidylinositol to Proteins and Cause Perinuclear ER-associated Actin Bar Formation. Mol. Biol. Cell 15: 2758-2770 [Abstract] [Full Text]
Moseley, J. B., Sagot, I., Manning, A. L., Xu, Y., Eck, M. J., Pellman, D., Goode, B. L. (2004). A Conserved Mechanism for Bni1- and mDia1-induced Actin Assembly and Dual Regulation of Bni1 by Bud6 and Profilin. Mol. Biol. Cell 15: 896-907 [Abstract] [Full Text]
Zakrzewska, E., Perron, M., Laroche, A., Pallotta, D. (2003). A Role for GEA1 and GEA2 in the Organization of the Actin Cytoskeleton in Saccharomyces cerevisiae. Genetics 165: 985-995 [Abstract] [Full Text]
Da Silva, J. S., Medina, M., Zuliani, C., Di Nardo, A., Witke, W., Dotti, C. G. (2003). RhoA/ROCK regulation of neuritogenesis via profilin IIa-mediated control of actin stability. JCB 162: 1267-1279 [Abstract] [Full Text]
Angermayr, M., Oechsner, U., Bandlow, W. (2003). Reb1p-dependent DNA Bending Effects Nucleosome Positioning and Constitutive Transcription at the Yeast Profilin Promoter. J. Biol. Chem. 278: 17918-17926 [Abstract] [Full Text]
McKinney, E. C., Kandasamy, M. K., Meagher, R. B. (2001). Small Changes in the Regulation of One Arabidopsis Profilin Isovariant, PRF1, Alter Seedling Development. Plant Cell 13: 1179-1191 [Abstract] [Full Text]
Lu, J., Pollard, T. D. (2001). Profilin Binding to Poly-L-Proline and Actin Monomers along with Ability to Catalyze Actin Nucleotide Exchange Is Required for Viability of Fission Yeast. Mol. Biol. Cell 12: 1161-1175 [Abstract] [Full Text]
Witke, W., Sutherland, J. D., Sharpe, A., Arai, M., Kwiatkowski, D. J. (2001). Profilin I is essential for cell survival and cell division in early mouse development. Proc. Natl. Acad. Sci. USA 10.1073/pnas.051515498v1 [Abstract] [Full Text]
Ozaki-Kuroda, K., Yamamoto, Y., Nohara, H., Kinoshita, M., Fujiwara, T., Irie, K., Takai, Y. (2001). Dynamic Localization and Function of Bni1p at the Sites of Directed Growth in Saccharomyces cerevisiae. Mol. Cell. Biol. 21: 827-839 [Abstract] [Full Text]
Whitacre, J. L., Davis, D. A., Toenjes, K. A., Brower, S. M., Adams, A. E. M. (2001). Generation of an Isogenic Collection of Yeast Actin Mutants and Identification of Three Interrelated Phenotypes. Genetics 157: 533-543 [Abstract] [Full Text]
Ooms, L. M., McColl, B. K., Wiradjaja, F., Wijayaratnam, A. P. W., Gleeson, P., Gething, M. J., Sambrook, J., Mitchell, C. A. (2000). The Yeast Inositol Polyphosphate 5-Phosphatases Inp52p and Inp53p Translocate to Actin Patches following Hyperosmotic Stress: Mechanism for Regulating Phosphatidylinositol 4,5-Bisphosphate at Plasma Membrane Invaginations. Mol. Cell. Biol. 20: 9376-9390 [Abstract] [Full Text]
Ramachandran, S., Christensen, H. E.M., Ishimaru, Y., Dong, C.-H., Chao-Ming, W., Cleary, A. L., Chua, N.-H. (2000). Profilin Plays a Role in Cell Elongation, Cell Shape Maintenance, and Flowering in Arabidopsis. Plant Physiol. 124: 1637-1647 [Abstract] [Full Text]
Freedman, T., Porter, A., Haarer, B. (2000). Mutational and hyperexpression-induced disruption of bipolar budding in yeast. Microbiology 146: 2833-2843 [Abstract] [Full Text]
Marcoux, N., Cloutier, S., Zakrzewska, E., Charest, P.-M., Bourbonnais, Y., Pallotta, D. (2000). Suppression of the Profilin-Deficient Phenotype by the RHO2 Signaling Pathway in Saccharomyces cerevisiae. Genetics 156: 579-592 [Abstract] [Full Text]
Wolven, A. K., Belmont, L. D., Mahoney, N. M., Almo, S. C., Drubin, D. G. (2000). In Vivo Importance of Actin Nucleotide Exchange Catalyzed by Profilin. JCB 150: 895-904 [Abstract] [Full Text]
Temesvari, L., Zhang, L., Fodera, B., Janssen, K.-P., Schleicher, M., Cardelli, J. A. (2000). Inactivation of lmpA, Encoding a LIMPII-related Endosomal Protein, Suppresses the Internalization and Endosomal Trafficking Defects in Profilin-null Mutants. Mol. Biol. Cell 11: 2019-2031 [Abstract] [Full Text]
Hoepfner, D., Brachat, A., Philippsen, P. (2000). Time-Lapse Video Microscopy Analysis Reveals Astral Microtubule Detachment in the Yeast Spindle Pole Mutant cnm67. Mol. Biol. Cell 11: 1197-1211 [Abstract] [Full Text]
Hughes, W. E., Woscholski, R., Cooke, F. T., Patrick, R. S., Dove, S. K., McDonald, N. Q., Parker, P. J. (2000). SAC1 Encodes a Regulated Lipid Phosphoinositide Phosphatase, Defects in Which Can Be Suppressed by the Homologous Inp52p and Inp53p Phosphatases. J. Biol. Chem. 275: 801-808 [Abstract] [Full Text]
Pruyne, D, Bretscher, A (2000). Polarization of cell growth in yeast. J. Cell Sci. 113: 571-585 [Abstract]
Ahern-Djamali, S. M., Bachmann, C., Hua, P., Reddy, S. K., Kastenmeier, A. S., Walter, U., Hoffmann, F. M. (1999). Identification of profilin and src homology 3�domains as binding partners for Drosophila Enabled. Proc. Natl. Acad. Sci. USA 96: 4977-4982 [Abstract] [Full Text]
Sandrock, T. M., Brower, S. M., Toenjes, K. A., Adams, A. E. M. (1999). Suppressor Analysis of Fimbrin (Sac6p) Overexpression in Yeast. Genetics 151: 1287-1297 [Abstract] [Full Text]
Reck-Peterson, S. L., Novick, P. J., Mooseker, M. S. (1999). The Tail of a Yeast Class V Myosin, Myo2p, Functions as a Localization Domain. Mol. Biol. Cell 10: 1001-1017 [Abstract] [Full Text]
Johnson, D. I. (1999). Cdc42: An Essential Rho-Type GTPase Controlling Eukaryotic Cell Polarity. Microbiol. Mol. Biol. Rev. 63: 54-105 [Abstract] [Full Text]
McMillan, J. N., Sia, R. A.L., Lew, D. J. (1998). A Morphogenesis Checkpoint Monitors the Actin Cytoskeleton in Yeast. JCB 142: 1487-1499 [Abstract] [Full Text]
Belmont, L. D., Drubin, D. G. (1998). The Yeast V159N Actin Mutant Reveals Roles for Actin Dynamics In Vivo. JCB 142: 1289-1299 [Abstract] [Full Text]
Homma, K., Terui, S., Minemura, M., Qadota, H., Anraku, Y., Kanaho, Y., Ohya, Y. (1998). Phosphatidylinositol-4-phosphate 5-Kinase Localized on the Plasma Membrane Is Essential for Yeast Cell Morphogenesis. J. Biol. Chem. 273: 15779-15786 [Abstract] [Full Text]
Anderson, B. L., Boldogh, I., Evangelista, M., Boone, C., Greene, L. A., Pon, L. A. (1998). The Src Homology Domain 3 (SH3) of a Yeast Type I Myosin, Myo5p, Binds to Verprolin and Is Required for Targeting to Sites of Actin Polarization. JCB 141: 1357-1370 [Abstract] [Full Text]
Yamashita, R. A., May, G. S. (1998). Constitutive Activation of Endocytosis by Mutation of myoA, the Myosin I Gene of Aspergillus nidulans. J. Biol. Chem. 273: 14644-14648 [Abstract] [Full Text]
Brachat, A., Kilmartin, J. V., Wach, A., Philippsen, P. (1998). Saccharomyces cerevisiae Cells with Defective Spindle Pole Body Outer Plaques Accomplish Nuclear Migration via Half-Bridge-organized Microtubules. Mol. Biol. Cell 9: 977-991 [Abstract] [Full Text]
Giansanti, M. G., Bonaccorsi, S., Williams, B., Williams, E. V., Santolamazza, C., Goldberg, M. L., Gatti, M. (1998). Cooperative interactions between the central spindle and the contractile ring during Drosophila cytokinesis. Genes Dev. 12: 396-410 [Abstract] [Full Text]
Schluter, K, Schleicher, M, Jockusch, B. (1998). Effects of single amino acid substitutions in the actin-binding site on the biological activity of bovine profilin I. J. Cell Sci. 111: 3261-3273 [Abstract]
Petersen, J, Nielsen, O, Egel, R, Hagan, I. (1998). F-actin distribution and function during sexual differentiation in Schizosaccharomyces pombe. J. Cell Sci. 111: 867-876 [Abstract]
Ayscough, K. R., Stryker, J., Pokala, N., Sanders, M., Crews, P., Drubin, D. G. (1997). High Rates of Actin Filament Turnover in Budding Yeast and Roles for Actin in Establishment and Maintenance of Cell Polarity Revealed Using the Actin Inhibitor Latrunculin-A. JCB 137: 399-416 [Abstract] [Full Text]
Evangelista, M., Blundell, K., Longtine, M. S., Chow, C. J., Adames, N., Pringle, J. R., Peter, M., Boone, C. (1997). Bni1p, a Yeast Formin Linking Cdc42p and the Actin Cytoskeleton During Polarized Morphogenesis. Science 276: 118-122 [Abstract] [Full Text]
Santos, B., Snyder, M. (1997). Targeting of Chitin Synthase 3 to Polarized Growth Sites in Yeast Requires Chs5p and Myo2p. JCB 136: 95-110 [Abstract] [Full Text]
Rothkegel, M, Mayboroda, O, Rohde, M, Wucherpfennig, C, Valenta, R, Jockusch, B. (1996). Plant and animal profilins are functionally equivalent and stabilize microfilaments in living animal cells. J. Cell Sci. 109: 83-90 [Abstract]
Chang, F, Woollard, A, Nurse, P (1996). Isolation and characterization of fission yeast mutants defective in the assembly and placement of the contractile actin ring. J. Cell Sci. 109: 131-142 [Abstract]
Ostrander, D. B., Gorman, J. A., Carman, G. M. (1995). Regulation of Profilin Localization in Saccharomyces cerevisiae by Phosphoinositide Metabolism. J. Biol. Chem. 270: 27045-27050 [Abstract] [Full Text]
Sohn, R. H., Chen, J., Koblan, K. S., Bray, P. F., Goldschmidt-Clermont, P. J. (1995). Localization of a Binding Site for Phosphatidylinositol 4,5-Bisphosphate on Human Profilin. J. Biol. Chem. 270: 21114-21120 [Abstract] [Full Text]
Freeman, N. L., Chen, Z., Horenstein, J., Weber, A., Field, J. (1995). An Actin Monomer Binding Activity Localizes to the Carboxyl-terminal Half of the Saccharomyces cerevisiae Cyclase-associated Protein. J. Biol. Chem. 270: 5680-5685 [Abstract] [Full Text]
Yu, G, Swiston, J, Young, D (1994). Comparison of human CAP and CAP2, homologs of the yeast adenylyl cyclase-associated proteins. J. Cell Sci. 107: 1671-1678 [Abstract]
Haarer, B., Petzold, A, Lillie, S., Brown, S. (1994). Identification of MYO4, a second class V myosin gene in yeast. J. Cell Sci. 107: 1055-1064 [Abstract]
Verheyen, E., Cooley, L (1994). Profilin mutations disrupt multiple actin-dependent processes during Drosophila development. Development 120: 717-728 [Abstract]
Witke, W., Sutherland, J. D., Sharpe, A., Arai, M., Kwiatkowski, D. J. (2001). Profilin I is essential for cell survival and cell division in early mouse development. Proc. Natl. Acad. Sci. USA 98: 3832-3836 [Abstract] [Full Text]