Antibodies directed against the peroxisomal targeting signal of firefly luciferase recognize multiple mammalian peroxisomal proteins

doi:10.1083/jcb.110.1.27

Quantitative Colocalization Analysis Software

This Article

	Full Text (PDF, 2175K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Gould, S. J.
	Articles by Subramani, S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Gould, S. J.
	Articles by Subramani, S.


Social Bookmarking

	What's this?

ARTICLES

Antibodies directed against the peroxisomal targeting signal of firefly luciferase recognize multiple mammalian peroxisomal proteins

SJ Gould, S Krisans, GA Keller and S Subramani
Department of Biology, University of California, La Jolla 92093.

We have previously shown that the peroxisomal targeting signal in firefly luciferase consists of the COOH-terminal three amino acids of the protein, serine-lysine-leucine (Gould, S.J., G.A. Keller, N. Hosken, J. Wilkinson, and S. Subramani, 1989. J. Cell Biol. 108:1657- 1664). Antibodies were raised against a synthetic peptide that contained this tripeptide at its COOH terminus. Immunofluorescence and immunocryoelectron microscopy revealed that the anti-peptide antibodies specifically detected peroxisomes in mammalian cells. Further characterization revealed that the antibodies were primarily directed against the COOH-terminal three amino acids of the peptide. In Western blot experiments, the antibodies recognized 15-20 rat liver peroxisomal proteins, but reacted with only a few proteins from other subcellular compartments. These results provide independent immunological evidence that the peroxisomal targeting signal identified in firefly luciferase is present in many peroxisomal proteins.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Pruimboom-Brees, I. M., Brees, D. J. J. E., Shen, A. C., Keener, M., Francone, O., Amacher, D. E., Loy, J. K., Kerlin, R. L. (2005). Using Laser Scanning Cytometry to Measure PPAR-Mediated Peroxisome Proliferation and {beta} Oxidation. Toxicol Pathol 33: 86-91 [Abstract] [Full Text]
Cheng, J. B., Russell, D. W. (2004). Mammalian Wax Biosynthesis: I. IDENTIFICATION OF TWO FATTY ACYL-COENZYME A REDUCTASES WITH DIFFERENT SUBSTRATE SPECIFICITIES AND TISSUE DISTRIBUTIONS. J. Biol. Chem. 279: 37789-37797 [Abstract] [Full Text]
Vizeacoumar, F. J., Torres-Guzman, J. C., Tam, Y. Y. C., Aitchison, J. D., Rachubinski, R. A. (2003). YHR150w and YDR479c encode peroxisomal integral membrane proteins involved in the regulation of peroxisome number, size, and distribution in Saccharomyces cerevisiae. JCB 161: 321-332 [Abstract] [Full Text]
Tam, Y. Y. C., Rachubinski, R. A. (2002). Yarrowia lipolytica Cells Mutant for the PEX24 Gene Encoding a Peroxisomal Membrane Peroxin Mislocalize Peroxisomal Proteins and Accumulate Membrane Structures Containing Both Peroxisomal Matrix and Membrane Proteins. Mol. Biol. Cell 13: 2681-2691 [Abstract] [Full Text]
Chang, C.-C., Warren, D. S., Sacksteder, K. A., Gould, S. J. (1999). Pex12 Interacts with Pex5 and Pex10 and Acts Downstream of Receptor Docking in Peroxisomal Matrix Protein Import. JCB 147: 761-774 [Abstract] [Full Text]
Geisbrecht, B. V., Gould, S. J. (1999). The Human PICD Gene Encodes a Cytoplasmic and Peroxisomal NADP+-dependent Isocitrate Dehydrogenase. J. Biol. Chem. 274: 30527-30533 [Abstract] [Full Text]
Gupta, S. D., Mehan, R. S., Tansey, T. R., Chen, H.-T., Goping, G., Goldberg, I., Shechter, I. (1999). Differential binding of proteins to peroxisomes in rat hepatoma cells: unique association of enzymes involved in isoprenoid metabolism. J. Lipid Res. 40: 1572-1584 [Abstract] [Full Text]
Geisbrecht, B. V., Zhang, D., Schulz, H., Gould, S. J. (1999). Characterization of PECI, a Novel Monofunctional Delta 3,Delta 2-Enoyl-CoA Isomerase of Mammalian Peroxisomes. J. Biol. Chem. 274: 21797-21803 [Abstract] [Full Text]
Schrader, M., Reuber, B. E., Morrell, J. C., Jimenez-Sanchez, G., Obie, C., Stroh, T. A., Valle, D., Schroer, T. A., Gould, S. J. (1998). Expression of PEX11beta Mediates Peroxisome Proliferation in the Absence of Extracellular Stimuli. J. Biol. Chem. 273: 29607-29614 [Abstract] [Full Text]
Engfelt, W. H., Shackelford, J. E., Aboushadi, N., Jessani, N., Masuda, K., Paton, V. G., Keller, G.-A., Krisans, S. K. (1997). Characterization of UT2 Cells. THE INDUCTION OF PEROXISOMAL 3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE. J. Biol. Chem. 272: 24579-24587 [Abstract] [Full Text]
Biardi, L., Krisans, S. K. (1996). Compartmentalization of Cholesterol Biosynthesis. J. Biol. Chem. 271: 1784-1788 [Abstract] [Full Text]
Slawecki, M., Dodt, G, Steinberg, S, Moser, A., Moser, H., Gould, S. (1995). Identification of three distinct peroxisomal protein import defects in patients with peroxisome biogenesis disorders. J. Cell Sci. 108: 1817-1829 [Abstract]
Wiemer, E.A.C., Terlecky, S.R., Nuttley, W.M., Subramani, S. (1995). Characterization of the Yeast and Human Receptors for the Carboxy-terminal Tripeptide Peroxisomal Targeting Signal. Cold Spring Harb Symp Quant Biol 60: 637-648 [Abstract]
Huhse, B., Kunau, W.-H. (1995). Protein Import into Peroxisomes: An Exception to the Rule?. Cold Spring Harb Symp Quant Biol 60: 657-662 [Abstract]